spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 2 March 2004
doi: 10.1242/jcs.01001

Journal of Cell Science 117, 1339-1350 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01001v1
117/8/1339    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mariner, D. J.
Right arrow Articles by Reynolds, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mariner, D. J.
Right arrow Articles by Reynolds, A. B.

Research Article

EGFR signaling to p120-catenin through phosphorylation at Y228

Deborah J. Mariner, Michael A. Davis and Albert B. Reynolds*

Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232-6840, USA

* Author for correspondence: (e-mail: al.reynolds{at}mcmail.vanderbilt.edu)

Accepted 18 November 2003

Epidermal growth factor receptor (EGFR) signals to p120ctn (p120), implying a role for EGFR in modulating cell-cell adhesion in epithelial tissues. p120 controls cadherin turnover, and may have other roles that modulate cadherin adhesiveness. To clarify the role for EGFR and other tyrosine kinases in regulating p120 function, we have generated and characterized a new phosphospecific antibody to p120 Y228, as well as a novel siRNA-based reconstitution system for analyzing roles of individual p120 phosphorylation events. In A431 cells, epidermal growth factor induced striking p120 phosphorylation at Y228. Y228-phosphorylated p120 localized to adherens junctions and lamellipodia, and was significantly enhanced in cells around the colony periphery. A screen of carcinoma cell lines revealed that some contain unusually high steady state levels of Y228 phosphorylation, suggesting that disregulated kinase activity in tumors may affect adhesion by constitutive cross talk to cadherin complexes. Despite these observations, mutation of Y228 and other prominent Src-associated p120 phosphorylation sites did not noticeably reduce the ability of E-cadherin to assemble junctions and induce compaction of cultured cells. Although A431 cells display significant activation of both EGFR and Src kinases, our data suggest that these account for only a fraction of the steady state activity that targets p120 Y228, and that Src family kinases are not necessary intermediates for epidermal growth factor-induced signaling to p120 Y228.

Key words: p120 catenin, Cadherin, Src, EGFR, Adherens junction, Adhesion




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
P. R. Molli, L. Adam, and R. Kumar
Therapeutic IMC-C225 Antibody Inhibits Breast Cancer Cell Invasiveness via Vav2-Dependent Activation of RhoA GTPase
Clin. Cancer Res., October 1, 2008; 14(19): 6161 - 6170.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. R. Ogden, L. E. Wroblewski, C. Weydig, J. Romero-Gallo, D. P. O'Brien, D. A. Israel, U. S. Krishna, B. Fingleton, A. B. Reynolds, S. Wessler, et al.
p120 and Kaiso Regulate Helicobacter pylori-induced Expression of Matrix Metalloproteinase-7
Mol. Biol. Cell, October 1, 2008; 19(10): 4110 - 4121.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ezaki, R.-J. Guo, H. Li, A. B. Reynolds, and J. P. Lynch
The homeodomain transcription factors Cdx1 and Cdx2 induce E-cadherin adhesion activity by reducing beta- and p120-catenin tyrosine phosphorylation
Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G54 - G65.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. I. Chernyavsky, J. Arredondo, Y. Kitajima, M. Sato-Nagai, and S. A. Grando
Desmoglein Versus Non-desmoglein Signaling in Pemphigus Acantholysis: CHARACTERIZATION OF NOVEL SIGNALING PATHWAYS DOWNSTREAM OF PEMPHIGUS VULGARIS ANTIGENS
J. Biol. Chem., May 4, 2007; 282(18): 13804 - 13812.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Castano, G. Solanas, D. Casagolda, I. Raurell, P. Villagrasa, X. R. Bustelo, A. Garcia de Herreros, and M. Dunach
Specific Phosphorylation of p120-Catenin Regulatory Domain Differently Modulates Its Binding to RhoA
Mol. Cell. Biol., March 1, 2007; 27(5): 1745 - 1757.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Bose, H. Molina, A. S. Patterson, J. K. Bitok, B. Periaswamy, J. S. Bader, A. Pandey, and P. A. Cole
Phosphoproteomic analysis of Her2/neu signaling and inhibition
PNAS, June 27, 2006; 103(26): 9773 - 9778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. A. Koop, M. F. B. G. Gebbink, T. E. Sweeney, M.-J. Mathy, H. F. G. Heijnen, J. A. E. Spaan, E. E. Voest, E. VanBavel, and S. L. M. Peters
Impaired flow-induced dilation in mesenteric resistance arteries from receptor protein tyrosine phosphatase-{micro}-deficient mice
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1218 - H1223.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. E. Moran, D. H. Hunt, S. H. Javid, M. Redston, A. M. Carothers, and M. M. Bertagnolli
Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice
J. Biol. Chem., October 8, 2004; 279(41): 43261 - 43272.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004