spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online March 12, 2004
doi: 10.1242/10.1242/jcs.00971

Journal of Cell Science 117, 1319-1328 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bilban, M.
Right arrow Articles by Desoye, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bilban, M.
Right arrow Articles by Desoye, G.

Research Article

Kisspeptin-10, a KiSS-1/metastin-derived decapeptide, is a physiological invasion inhibitor of primary human trophoblasts

Martin Bilban1,2,3,*, Nassim Ghaffari-Tabrizi2,*, Edith Hintermann1, Sandra Bauer5, Sylvia Molzer3, Cristina Zoratti4, Roland Malli4, Andrew Sharabi1, Ursula Hiden2, Wolfgang Graier4, Martin Knöfler5, Fritz Andreae2, Oswald Wagner3, Vito Quaranta1 and Gernot Desoye2,{ddagger}

1 Department of Cell Biology, The Scripps Research Institute, North Torrey Pines Road, La Jolla, 92037 CA, USA
2 Clinic of Obstetrics and Gynecology, Karl Franzens University Graz, Auenbruggerplatz 14, 8036 Graz, Austria
3 Institute for Laboratory Medicine, University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria
5 Clinic of Obstetrics and Gynecology, University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria
4 Institute for Medical Biochemistry, Karl-Franzens-University of Graz, Harrachgasse 21/III, 8010 Graz, Austria

{ddagger} Author for correspondence (e-mail: gernot.desoye{at}kfunigraz.ac.at)

Accepted 5 November 2003

Trophoblast invasion of the uterine extracellular matrix, a critical process of human implantation and essential for fetal development, is a striking example of controlled invasiveness. To identify molecules that regulate trophoblast invasion, mRNA signatures of trophoblast cells isolated from first trimester (high invasiveness) and term placentae (no/low invasiveness) were compared using U95A GeneChip microarrays yielding 220 invasion/migration-related genes. In this `invasion cluster', KiSS-1 and its G-protein-coupled receptor KiSS-1R were expressed at higher levels in first trimester trophoblasts than at term of gestation. Receptor and ligand mRNA and protein were localized to the trophoblast compartment. In contrast to KiSS-1, which is only expressed in the villous trophoblast, KiSS-1R was also found in the extravillous trophoblast, suggesting endocrine/paracrine activation mechanisms. The primary translation product of KiSS-1 is a 145 amino acid polypeptide (Kp-145), but shorter kisspeptins (Kp) with 10, 13, 14 or 54 amino acid residues may be produced. We identified Kp-10, a dekapeptide derived from the primary translation product, in conditioned medium of first trimester human trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 inhibited trophoblast migration in an explant as well as transwell assay without affecting proliferation. Suppressed motility was paralleled with suppressed gelatinolytic activity of isolated trophoblasts. These results identifed Kp-10 as a novel paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself.

Key words: Invasion, DNA microarray, Kisspeptins, Metastin, Trophoblast




This article has been cited by other articles:


Home page
 ReproductionHome page
E. Gianetti and S. Seminara
Kisspeptin and KISS1R: a critical pathway in the reproductive system
Reproduction, September 1, 2008; 136(3): 295 - 301.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
R A Silvestre, E M Egido, R Hernandez, and J Marco
Kisspeptin-13 inhibits insulin secretion without affecting glucagon or somatostatin release: study in the perfused rat pancreas
J. Endocrinol., February 1, 2008; 196(2): 283 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
Y. Nakamura, S. Aoki, Yewei Xing, H. Sasano, and W. E. Rainey
Metastin Stimulates Aldosterone Synthesis in Human Adrenal Cells
Reproductive Sciences, December 1, 2007; 14(8): 836 - 845.
[Abstract] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Quaynor, L. Hu, P. K. Leung, H. Feng, N. Mores, L. Z. Krsmanovic, and K. J. Catt
Expression of a Functional G Protein-Coupled Receptor 54-Kisspeptin Autoregulatory System in Hypothalamic Gonadotropin-Releasing Hormone Neurons
Mol. Endocrinol., December 1, 2007; 21(12): 3062 - 3070.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
W. S. Dhillo, O. B. Chaudhri, E. L. Thompson, K. G. Murphy, M. Patterson, R. Ramachandran, G. K. Nijher, V. Amber, A. Kokkinos, M. Donaldson, et al.
Kisspeptin-54 Stimulates Gonadotropin Release Most Potently during the Preovulatory Phase of the Menstrual Cycle in Women
J. Clin. Endocrinol. Metab., October 1, 2007; 92(10): 3958 - 3966.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Lapatto, J. C. Pallais, D. Zhang, Y.-M. Chan, A. Mahan, F. Cerrato, W. W. Le, G. E. Hoffman, and S. B. Seminara
Kiss1 / Mice Exhibit More Variable Hypogonadism than Gpr54 / Mice
Endocrinology, October 1, 2007; 148(10): 4927 - 4936.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Li, D. Mitchell, J. Luo, Z. Yi, S.-G. Cho, J. Guo, X. Li, G. Ning, X. Wu, and M. Liu
Estrogen Regulates KiSS1 Gene Expression through Estrogen Receptor {alpha} and SP Protein Complexes
Endocrinology, October 1, 2007; 148(10): 4821 - 4828.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
D. Marot, I. Bieche, C. Aumas, S. Esselin, C. Bouquet, S. Vacher, G. Lazennec, M. Perricaudet, F. Kuttenn, R. Lidereau, et al.
High tumoral levels of Kiss1 and G-protein-coupled receptor 54 expression are correlated with poor prognosis of estrogen receptor-positive breast tumors
Endocr. Relat. Cancer, September 1, 2007; 14(3): 691 - 702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Maruyama, A. Hattori, Y. Goto, M. Ueda, M. Maeda, H. Fujiwara, and M. Tsujimoto
Laeverin/Aminopeptidase Q, a Novel Bestatin-sensitive Leucine Aminopeptidase Belonging to the M1 Family of Aminopeptidases
J. Biol. Chem., July 13, 2007; 282(28): 20088 - 20096.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. d'Anglemont de Tassigny, L. A. Fagg, J. P. C. Dixon, K. Day, H. G. Leitch, A. G. Hendrick, D. Zahn, I. Franceschini, A. Caraty, M. B. L. Carlton, et al.
Hypogonadotropic hypogonadism in mice lacking a functional Kiss1 gene
PNAS, June 19, 2007; 104(25): 10714 - 10719.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
C. Ferretti, L. Bruni, V. Dangles-Marie, A.P. Pecking, and D. Bellet
Molecular circuits shared by placental and cancer cells, and their implications in the proliferative, invasive and migratory capacities of trophoblasts
Hum. Reprod. Update, March 1, 2007; 13(2): 121 - 141.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
K. T. Nash, P. A. Phadke, J.-M. Navenot, D. R. Hurst, M. A. Accavitti-Loper, E. Sztul, K. S. Vaidya, A. R. Frost, J. C. Kappes, S. C. Peiper, et al.
Requirement of KISS1 Secretion for Multiple Organ Metastasis Suppression and Maintenance of Tumor Dormancy
J Natl Cancer Inst, February 21, 2007; 99(4): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. J. Mead, J. J. Maguire, R. E. Kuc, and A. P. Davenport
Kisspeptins Are Novel Potent Vasoconstrictors in Humans, with a Discrete Localization of Their Receptor, G Protein-Coupled Receptor 54, to Atherosclerosis-Prone Vessels
Endocrinology, January 1, 2007; 148(1): 140 - 147.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
W. S. Dhillo, P. Savage, K. G. Murphy, O. B. Chaudhri, M. Patterson, G. M. Nijher, V. M. Foggo, G. S. Dancey, H. Mitchell, M. J. Seckl, et al.
Plasma kisspeptin is raised in patients with gestational trophoblastic neoplasia and falls during treatment
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E878 - E884.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. Castellano, M. Gaytan, J. Roa, E. Vigo, V. M. Navarro, C. Bellido, C. Dieguez, E. Aguilar, J. E. Sanchez-Criado, A. Pellicer, et al.
Expression of KiSS-1 in Rat Ovary: Putative Local Regulator of Ovulation?
Endocrinology, October 1, 2006; 147(10): 4852 - 4862.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
M. Tena-Sempere
GPR54 and kisspeptin in reproduction
Hum. Reprod. Update, September 1, 2006; 12(5): 631 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Lucerna, J. Pomyje, D. Mechtcheriakova, A. Kadl, F. Gruber, M. Bilban, Y. Sobanov, G. Schabbauer, J. Breuss, O. Wagner, et al.
Sustained Expression of Early Growth Response Protein-1 Blocks Angiogenesis and Tumor Growth.
Cancer Res., July 1, 2006; 66(13): 6708 - 6713.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. M. Dungan, D. K Clifton, and R. A. Steiner
Minireview: Kisspeptin Neurons as Central Processors in the Regulation of Gonadotropin-Releasing Hormone Secretion
Endocrinology, March 1, 2006; 147(3): 1154 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Pompolo, A. Pereira, K. M. Estrada, and I. J. Clarke
Colocalization of Kisspeptin and Gonadotropin-Releasing Hormone in the Ovine Brain
Endocrinology, February 1, 2006; 147(2): 804 - 810.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. B. Seminara
We All Remember Our First Kiss: Kisspeptin and the Male Gonadal Axis
J. Clin. Endocrinol. Metab., December 1, 2005; 90(12): 6738 - 6740.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
W. S. Dhillo, O. B. Chaudhri, M. Patterson, E. L. Thompson, K. G. Murphy, M. K. Badman, B. M. McGowan, V. Amber, S. Patel, M. A. Ghatei, et al.
Kisspeptin-54 Stimulates the Hypothalamic-Pituitary Gonadal Axis in Human Males
J. Clin. Endocrinol. Metab., December 1, 2005; 90(12): 6609 - 6615.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J.-M. Navenot, Z. Wang, M. Chopin, N. Fujii, and S. C. Peiper
Kisspeptin-10-Induced Signaling of GPR54 Negatively Regulates Chemotactic Responses Mediated by CXCR4: a Potential Mechanism for the Metastasis Suppressor Activity of Kisspeptins
Cancer Res., November 15, 2005; 65(22): 10450 - 10456.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. C. Arai, Y.-F. Xia, E. Suzuki, M. Kessler, O. Civelli, and H.-P. Nothacker
Cancer Metastasis-Suppressing Peptide Metastin Upregulates Excitatory Synaptic Transmission in Hippocampal Dentate Granule Cells
J Neurophysiol, November 1, 2005; 94(5): 3648 - 3652.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. M. Navarro, J. M. Castellano, R. Fernandez-Fernandez, S. Tovar, J. Roa, A. Mayen, M. L. Barreiro, F. F. Casanueva, E. Aguilar, C. Dieguez, et al.
Effects of KiSS-1 Peptide, the Natural Ligand of GPR54, on Follicle-Stimulating Hormone Secretion in the Rat
Endocrinology, April 1, 2005; 146(4): 1689 - 1697.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Messager, E. E. Chatzidaki, D. Ma, A. G. Hendrick, D. Zahn, J. Dixon, R. R. Thresher, I. Malinge, D. Lomet, M. B. L. Carlton, et al.
Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54
PNAS, February 1, 2005; 102(5): 1761 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. M. Navarro, J. M. Castellano, R. Fernandez-Fernandez, S. Tovar, J. Roa, A. Mayen, R. Nogueiras, M. J. Vazquez, M. L. Barreiro, P. Magni, et al.
Characterization of the Potent Luteinizing Hormone-Releasing Activity of KiSS-1 Peptide, the Natural Ligand of GPR54
Endocrinology, January 1, 2005; 146(1): 156 - 163.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. M Navarro, R Fernandez-Fernandez, J. M Castellano, J Roa, A Mayen, M. L Barreiro, F Gaytan, E Aguilar, L Pinilla, C Dieguez, et al.
Advanced vaginal opening and precocious activation of the reproductive axis by KiSS-1 peptide, the endogenous ligand of GPR54
J. Physiol., December 1, 2004; 561(2): 379 - 386.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004