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First published online 6 January 2004
doi: 10.1242/jcs.00879

Journal of Cell Science 117, 609-618 (2004)
Published by The Company of Biologists 2004
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Research Article

The tyrosine phosphatase DEP-1 induces cytoskeletal rearrangements, aberrant cell-substratum interactions and a reduction in cell proliferation

Stuart Kellie1,2,*, Graham Craggs2,4, Ian N. Bird2,5 and Gareth E. Jones3

1 School of Molecular and Microbial Sciences, Institute for Molecular Bioscience and CRC for Chronic Inflammatory Diseases, University of Queensland, Brisbane, QLD 4072, Australia
2 Yamanouchi Research Institute, Littlemore Park, Armstrong Road, Oxford OX4 4XS, UK
3 Randall Centre, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK
4 OSI Pharmaceuticals, Watlington Road, Oxford, OX4 6LT, UK
5 Oxford Glycosciences plc, The Forum, 86 Milton Park, Abingdon, Oxon OX14, UK

* Author for correspondence (e-mail: s.kellie{at}mailbox.uq.edu.au)

Accepted 12 September 2003

The receptor protein tyrosine phosphatase density-enhanced phosphatase-1 (DEP-1) has been implicated in aberrant cancer cell growth and immune cell function, however, its function within cells has yet to be properly elucidated. To investigate the cellular function of DEP-1, stable cell lines inducibly expressing DEP-1 were generated. Induction of DEP-1 expression was found to decrease PDGF-stimulated tyrosine phosphorylation of a number of cellular proteins including the PDGF receptor, and to inhibit growth factor-stimulated phosphorylation of components of the MAPK pathway, indicating that DEP-1 antagonised PDGF receptor signalling. This was supported by data showing that DEP-1 expression resulted in a reduction in cell proliferation. DEP-1-expressing cells had fewer actin-containing microfilament bundles, reduced vinculin and paxillin-containing adhesion plaques, and were defective in interactions with fibronectin. Defective cell-substratum adhesion correlated with lack of activation of FAK in DEP-1-expressing cells. Time-lapse interference reflection microscopy of live cells revealed that although small focal contacts at the leading edge were generated in DEP-1-expressing cells, they failed to mature into stable focal adhesions, as found in control cells. Further motility analysis revealed that DEP-1-expressing cells retained limited random motility, but showed no chemotaxis towards a gradient of PDGF. In addition, cell-cell contacts were disrupted, with a change in the localisation of cadherin from discrete areas of cell-cell contact to large areas of membrane interaction, and there was a parallel redistribution of ß-catenin. These results demonstrate that DEP-1 is a negative regulator of cell proliferation, cell-substratum contacts, motility and chemotaxis in fibroblasts.

Key words: Tyrosine phosphatase, Cytoskeleton, Motility


Related articles in JCS:

DEP-1: a brake on cell growth

JCS 2004 117: 401. [Full Text]  



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