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First published online 26 October 2004
doi: 10.1242/jcs.01499

Journal of Cell Science 117, 5759-5770 (2004)
Published by The Company of Biologists 2004
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Research Article

Translocation of the Dictyostelium TRAP1 homologue to mitochondria induces a novel prestarvation response

Tsuyoshi Morita*, Aiko Amagai and Yasuo Maeda{ddagger}

Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980-8578, Japan

{ddagger} Author for correspondence (e-mail: ymaeda{at}mail.tains.tohoku.ac.jp)

Accepted 17 August 2004

Dd-TRAP1 is a Dictyostelium homologue of tumor necrosis factor receptor-associated protein 1 (TRAP-1). Dd-TRAP1 is located in the cortex of cells growing at a low density, but was found to be translocated to mitochondria with the help of a novel prestarvation factor that was accumulated in growth medium along with increased cell densities. The knockdown mutant of Dd-TRAP1 (TRAP1-RNAi cells) exhibited a significant defect in prestarvation response. Although TRAP1-RNAi cells showed normal expressions of classical prestarvation genes [dscA (discoidin I) and car1 (carA; cAMP receptor)], the expression of differentiation-associated genes (dia1 and dia3) induced by the prestarvation response were markedly repressed. By contrast, transformants overexpressing Dd-TRAP1 showed an early prestarvation response and also increased expression of dia1 and dia3 in a cell-density-dependent manner. Importantly, introduction of Dd-TRAP1 antibody into D. discoideum Ax-2 cells by electroporation inhibited the translocation of Dd-TRAP1 from the cortex to mitochondria and greatly inhibited the initiation of differentiation. Taken together, these results indicate that Dd-TRAP1 is translocated to mitochondria by sensing the cell density in growth medium and enhances the early developmental program through a novel prestarvation response.

Key words: TRAP-1, Hsp90, Differentiation, Prestarvation response, Mitochondria, Dictyostelium




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[Abstract] [Full Text] [PDF]


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