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First published online 28 September 2004
doi: 10.1242/jcs.01403

Journal of Cell Science 117, 5209-5220 (2004)
Published by The Company of Biologists 2004
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Research Article

Tetraspanin protein (TSP-15) is required for epidermal integrity in Caenorhabditis elegans

Hiroki Moribe1,*, John Yochem2,*, Hiromi Yamada1, Yo Tabuse3, Toyoshi Fujimoto4 and Eisuke Mekada1,{ddagger}

1 Department of Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
2 Department of Genetics, Cell Biology, and Development, University of Minnesota, 420 Washington Avenue, SE Minneapolis, MN 55455, USA
3 Fundamental Research Laboratories, NEC Corporation, 34 Miyukigaoka, Tsukuba, Ibaraki 305-8501, Japan
4 Department of Anatomy and Molecular Cell Biology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan

{ddagger} Author for correspondence (e-mail: emekada{at}biken.osaka-u.ac.jp)

Accepted 8 July 2004

Epidermal integrity is essential for animal development and survival. Here, we demonstrate that TSP-15, a member of the tetraspanin protein family, is required for epithelial membrane integrity in Caenorhabditis elegans. Reduction of tsp-15 function by mutation or by RNA interference elicits abnormalities of the hypodermis, including dissociation of the cuticle and degeneration of the hypodermis. Lethality during molting often results. Examination of GFP transgenic animals, genetic mosaic analysis and rescue assays revealed that TSP-15 functions in hyp7, a large syncytium that composes most of the hypodermis. Assays with a membrane-impermeable dye or leakage analysis of a hypodermal-specific marker indicate that the barrier function of the hypodermal membrane is impaired owing to the loss or reduction of TSP-15. These results indicate that TSP-15 functions in the maintenance of epithelial cell integrity.

Key words: Tetraspanin, TSP-15, Epidermis, Cuticle, Integrity


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© The Company of Biologists Ltd 2004