spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online April 16, 2004
doi: 10.1242/10.1242/jcs.01034

Journal of Cell Science 117, 1911-1922 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bharathi, V.
Right arrow Articles by Shashidhara, L. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bharathi, V.
Right arrow Articles by Shashidhara, L. S.

Research Article

Genetic characterization of the Drosophila homologue of coronin

V. Bharathi, S. K. Pallavi, R. Bajpai, B. S. Emerald and L. S. Shashidhara*

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India 500 007

* Author for correspondence (e-mail: shashi{at}ccmb.res.in)

Accepted 3 December 2003

We report cloning and characterization of coro, which codes for the Drosophila homologue of the F-actin binding protein coronin. Viable alleles of coro produce a variety of phenotypes in leg, wing and eye development, which are similar to the phenotypes observed as a result of mutations in genes associated with the actin cytoskeleton and/or membrane trafficking. Homozygous lethal mutations in coro results in the disruption of the actin cytoskeleton in wing imaginal discs. Formation of both basolateral septate junctions and apical adherens junctions are also adversely affected in epithelial cells. Both viable and lethal alleles of coro show genetic interactions with syntaxin1A, a gene required for membrane trafficking. They also show enhanced response to over-expression of Decapentaplegic (Dpp) and its receptor Thick vein. Tracing of Dpp morphogen using a Dpp::GFP fusion construct suggested defects in the endocytic pathway, which resulted in uniform distribution of Dpp along the AP axis rather than a gradient from the AP boundary. Our results provide a genetic link between endocytosis/exocytosis events involving F actin-coated vesicles and the establishment of morphogen gradient.

Key words: Coronin, Drosophila melanogaster, Anteroposterior boundary, Dpp signaling, Membrane trafficking




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. Cai, N. Holoweckyj, M. D. Schaller, and J. E. Bear
Phosphorylation of Coronin 1B by Protein Kinase C Regulates Interaction with Arp2/3 and Cell Motility
J. Biol. Chem., September 9, 2005; 280(36): 31913 - 31923.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. K. Pallavi and L. S. Shashidhara
Signaling interactions between squamous and columnar epithelia of the Drosophila wing disc
J. Cell Sci., August 1, 2005; 118(15): 3363 - 3370.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004