QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online December 1, 2003
doi: 10.1242/10.1242/jcs.00848

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fahrenkrog, B. Articles by Aebi, U. Search for Related Content PubMed PubMed Citation Articles by Fahrenkrog, B. Articles by Aebi, U.

The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis

M. E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Klingelbergstr. 70, 4056 Basel, Switzerland

^* Author for correspondence (e-mail: birthe.fahrenkrog{at}unibas.ch)

Accepted 27 August 2003

The yeast S. cerevisiae can undergo programmed cell death that exhibits the typical cellular markers of apoptosis. The mammalian HtrA2 protein was recently reported to mediate apoptosis in a serine-protease-dependent manner owing to its ability to antagonise the inhibitor of apoptosis protein XIAP. Here, we report the identification and characterisation of the S. cerevisiae HtrA-like protein, which we termed Nma111p (for nuclear mediator of apoptosis), as a mediator of yeast apoptosis. Nma111p is a nuclear protein that, under cellular stress conditions (i.e. at elevated temperature or after induction of apoptosis by H₂O₂), tends to aggregate inside the nucleus without its expression level being upregulated, suggesting that aggregation of Nma111p is correlated to its death-mediating character. Nma111p belongs to the HtrA family of serine proteases and its pro-apoptotic activity depends on its serine-protease activity. Yeast cells that lack Nma111p survive better at 50°C than wild-type cells and the cells show no apoptotic hallmarks, such as chromatin condensation and fragmentation, or accumulation of reactive oxygen species, after the induction of apoptosis by H₂O₂. By contrast, overexpression of Nma111p enhances apoptotic-like cell death. Therefore, Nma111p, like its mammalian homologue HtrA2, mediates apoptosis.

Key words: Apoptosis, HtrA, Nic96p, Nucleus, Yeast

This article has been cited by other articles:

				R. Guerin, G. Arseneault, S. Dumont, and L. A. Rokeach Calnexin Is Involved in Apoptosis Induced by Endoplasmic Reticulum Stress in the Fission Yeast Mol. Biol. Cell, October 1, 2008; 19(10): 4404 - 4420. [Abstract] [Full Text] [PDF]

				K. Sapienza, W. Bannister, and R. Balzan Mitochondrial involvement in aspirin-induced apoptosis in yeast Microbiology, September 1, 2008; 154(9): 2740 - 2747. [Abstract] [Full Text] [PDF]

				P. Hauptmann and L. Lehle Kex1 Protease Is Involved in Yeast Cell Death Induced by Defective N-Glycosylation, Acetic Acid, and Chronological Aging J. Biol. Chem., July 4, 2008; 283(27): 19151 - 19163. [Abstract] [Full Text] [PDF]

				S. Mroczek and J. Kufel Apoptotic signals induce specific degradation of ribosomal RNA in yeast Nucleic Acids Res., May 1, 2008; 36(9): 2874 - 2888. [Abstract] [Full Text] [PDF]

				H. Yang, Q. Ren, and Z. Zhang Cleavage of Mcd1 by Caspase-like Protease Esp1 Promotes Apoptosis in Budding Yeast Mol. Biol. Cell, May 1, 2008; 19(5): 2127 - 2134. [Abstract] [Full Text] [PDF]

				S. Buttner, A. Bitto, J. Ring, M. Augsten, P. Zabrocki, T. Eisenberg, H. Jungwirth, S. Hutter, D. Carmona-Gutierrez, G. Kroemer, et al. Functional Mitochondria Are Required for {alpha}-Synuclein Toxicity in Aging Yeast J. Biol. Chem., March 21, 2008; 283(12): 7554 - 7560. [Abstract] [Full Text] [PDF]

				O. Odat, S. Matta, H. Khalil, S. C. Kampranis, R. Pfau, P. N. Tsichlis, and A. M. Makris Old Yellow Enzymes, Highly Homologous FMN Oxidoreductases with Modulating Roles in Oxidative Stress and Programmed Cell Death in Yeast J. Biol. Chem., December 7, 2007; 282(49): 36010 - 36023. [Abstract] [Full Text] [PDF]

				Q. Liang and B. Zhou Copper and Manganese Induce Yeast Apoptosis via Different Pathways Mol. Biol. Cell, December 1, 2007; 18(12): 4741 - 4749. [Abstract] [Full Text] [PDF]

				M. Helm, C. Luck, J. Prestele, G. Hierl, P. F. Huesgen, T. Frohlich, G. J. Arnold, I. Adamska, A. Gorg, F. Lottspeich, et al. Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants PNAS, July 3, 2007; 104(27): 11501 - 11506. [Abstract] [Full Text] [PDF]

				X.-P. Li, M. Baricevic, H. Saidasan, and N. E. Tumer Ribosome Depurination Is Not Sufficient for Ricin-Mediated Cell Death in Saccharomyces cerevisiae Infect. Immun., January 1, 2007; 75(1): 417 - 428. [Abstract] [Full Text] [PDF]

				S. Buttner, T. Eisenberg, E. Herker, D. Carmona-Gutierrez, G. Kroemer, and F. Madeo Why yeast cells can undergo apoptosis: death in times of peace, love, and war J. Cell Biol., November 20, 2006; 175(4): 521 - 525. [Abstract] [Full Text] [PDF]

				G. F. Ribeiro, M. Corte-Real, and B. Johansson Characterization of DNA Damage in Yeast Apoptosis Induced by Hydrogen Peroxide, Acetic Acid, and Hyperosmotic Shock Mol. Biol. Cell, October 1, 2006; 17(10): 4584 - 4591. [Abstract] [Full Text] [PDF]

				C. Allen, S. Buttner, A. D. Aragon, J. A. Thomas, O. Meirelles, J. E. Jaetao, D. Benn, S. W. Ruby, M. Veenhuis, F. Madeo, et al. Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures J. Cell Biol., July 3, 2006; 174(1): 89 - 100. [Abstract] [Full Text] [PDF]

				D. Walter, S. Wissing, F. Madeo, and B. Fahrenkrog The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2 J. Cell Sci., May 1, 2006; 119(9): 1843 - 1851. [Abstract] [Full Text] [PDF]

				W. Li, L. Sun, Q. Liang, J. Wang, W. Mo, and B. Zhou Yeast AMID Homologue Ndi1p Displays Respiration-restricted Apoptotic Activity and Is Involved in Chronological Aging Mol. Biol. Cell, April 1, 2006; 17(4): 1802 - 1811. [Abstract] [Full Text] [PDF]

				M. Weinberger, L. Ramachandran, L. Feng, K. Sharma, X. Sun, M. Marchetti, J. A. Huberman, and W. C. Burhans Apoptosis in budding yeast caused by defects in initiation of DNA replication J. Cell Sci., August 1, 2005; 118(15): 3543 - 3553. [Abstract] [Full Text] [PDF]

				N. Watanabe and E. Lam Two Arabidopsis Metacaspases AtMCP1b and AtMCP2b Are Arginine/Lysine-specific Cysteine Proteases and Activate Apoptosis-like Cell Death in Yeast J. Biol. Chem., April 15, 2005; 280(15): 14691 - 14699. [Abstract] [Full Text] [PDF]

				J. Reiter, E. Herker, F. Madeo, and M. J. Schmitt Viral killer toxins induce caspase-mediated apoptosis in yeast J. Cell Biol., January 31, 2005; 168(3): 353 - 358. [Abstract] [Full Text] [PDF]

				A. I. Pozniakovsky, D. A. Knorre, O. V. Markova, A. A. Hyman, V. P. Skulachev, and F. F. Severin Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast J. Cell Biol., January 17, 2005; 168(2): 257 - 269. [Abstract] [Full Text] [PDF]

				R. J. Davenport Culture Clash Sci. Aging Knowl. Environ., November 17, 2004; 2004(46): ns9 - ns9. [Abstract] [Full Text] [PDF]

				F. Abdel-Sater, M. El Bakkoury, A. Urrestarazu, S. Vissers, and B. Andre Amino Acid Signaling in Yeast: Casein Kinase I and the Ssy5 Endoprotease Are Key Determinants of Endoproteolytic Activation of the Membrane-Bound Stp1 Transcription Factor Mol. Cell. Biol., November 15, 2004; 24(22): 9771 - 9785. [Abstract] [Full Text] [PDF]

				S. Wissing, P. Ludovico, E. Herker, S. Buttner, S. M. Engelhardt, T. Decker, A. Link, A. Proksch, F. Rodrigues, M. Corte-Real, et al. An AIF orthologue regulates apoptosis in yeast J. Cell Biol., October 4, 2004; (2004) jcb.200404138. [Abstract] [Full Text] [PDF]