|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 5 August 2003
doi: 10.1242/jcs.00665
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Department of Anatomy, Cell and Human Biology, Guy's, King's and St Thomas'
School of Biomedical Science, Guy's Hospital, London SE1 1UL, UK
2 Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine,
Guy's Hospital, London SE1 9RT, UK
3 Division of Parasitology, National Institute for Medical Research, Mill Hill,
London NW7 1AA, UK
4 Department of Parasitology, Biomedical Primate Research Centre, Lange Kleiweg,
2280GH, Rijswijk, The Netherlands
* Author for correspondence (e-mail: lawrence.bannister{at}kcl.ac.uk)
Accepted 12 May 2003
During the assembly of Plasmodium falciparum merozoites within the schizont stage, the parasite synthesizes and positions three sets of secretory vesicles (rhoptries, micronemes and dense granules) that are active during red cell invasion. There are up to 40 micronemes per merozoite, shaped like long-necked bottles, about 160 nm long and 65 nm at their widest diameter. On their external surfaces, they bear bristle-like filaments, each 3-4 nm thick and 25 nm long. Micronemes are translocated from a single Golgi-like cisterna near the nucleus along a band of two or three subpellicular microtubules to the merozoite apex, where they dock with the rhoptry tips. Dense granules are also formed around the periphery of the Golgi cisternae but their distribution is unrelated to microtubules. Three polyclonal antibodies raised against the recombinant PfAMA-1 ectodomain sequence recognizing both the 83 kDa and processed 66 kDa molecules label the peripheries of translocating and mature micronemes but do not label rhoptries significantly at any stage of merozoite development within schizonts. This result confirms that PfAMA-1 is a micronemal protein, and indicates that within the microneme it is located near or inserted into this organelle's boundary membrane.
Key words: AMA-1, Electron microscopy, f-MAST, Merozoite, Microneme, Microtubule, Plasmodium falciparum, Trafficking
This article has been cited by other articles:
|
|
 |
|
  J. L. Green, R. R. Rees-Channer, S. A. Howell, S. R. Martin, E. Knuepfer, H. M. Taylor, M. Grainger, and A. A. Holder The Motor Complex of Plasmodium falciparum: PHOSPHORYLATION BY A CALCIUM-DEPENDENT PROTEIN KINASE J. Biol. Chem., November 7, 2008; 283(45): 30980 - 30989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Struck, S. Herrmann, C. Langer, A. Krueger, B. J. Foth, K. Engelberg, A. L. Cabrera, S. Haase, M. Treeck, M. Marti, et al. Plasmodium falciparum possesses two GRASP proteins that are differentially targeted to the Golgi complex via a higher- and lower-eukaryote-like mechanism J. Cell Sci., July 1, 2008; 121(13): 2123 - 2129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Singh, M. Plassmeyer, D. Gaur, and L. H. Miller Mononeme: A new secretory organelle in Plasmodium falciparum merozoites identified by localization of rhomboid-1 protease PNAS, December 11, 2007; 104(50): 20043 - 20048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Miura, H. Zhou, O. V. Muratova, A. C. Orcutt, B. Giersing, L. H. Miller, and C. A. Long In Immunization with Plasmodium falciparum Apical Membrane Antigen 1, the Specificity of Antibodies Depends on the Species Immunized Infect. Immun., December 1, 2007; 75(12): 5827 - 5836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Collins, C. Withers-Martinez, G. A. Bentley, A. H. Batchelor, A. W. Thomas, and M. J. Blackman Fine Mapping of an Epitope Recognized by an Invasion-inhibitory Monoclonal Antibody on the Malaria Vaccine Candidate Apical Membrane Antigen 1 J. Biol. Chem., March 9, 2007; 282(10): 7431 - 7441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Treeck, N. S. Struck, S. Haase, C. Langer, S. Herrmann, J. Healer, A. F. Cowman, and T. W. Gilberger A Conserved Region in the EBL Proteins Is Implicated in Microneme Targeting of the Malaria Parasite Plasmodium falciparum J. Biol. Chem., October 20, 2006; 281(42): 31995 - 32003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. MORAIS, I. S. SOARES, L. H. CARVALHO, C. J. F. FONTES, A. U. KRETTLI, and E. M. BRAGA ANTIBODIES TO PLASMODIUM VIVAX APICAL MEMBRANE ANTIGEN 1: PERSISTENCE AND CORRELATION WITH MALARIA TRANSMISSION INTENSITY. Am J Trop Med Hyg, October 1, 2006; 75(4): 582 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Alexander, S. Arastu-Kapur, J.-F. Dubremetz, and J. C. Boothroyd Plasmodium falciparum AMA1 Binds a Rhoptry Neck Protein Homologous to TgRON4, a Component of the Moving Junction in Toxoplasma gondii. Eukaryot. Cell, July 1, 2006; 5(7): 1169 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Cooke, D. W. Buckingham, F. K. Glenister, K. M. Fernandez, L. H. Bannister, M. Marti, N. Mohandas, and R. L. Coppel A Maurer's cleft-associated protein is essential for expression of the major malaria virulence antigen on the surface of infected red blood cells J. Cell Biol., March 13, 2006; 172(6): 899 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Struck, S. de Souza Dias, C. Langer, M. Marti, J. A. Pearce, A. F. Cowman, and T. W. Gilberger Re-defining the Golgi complex in Plasmodium falciparum using the novel Golgi marker PfGRASP J. Cell Sci., December 1, 2005; 118(23): 5603 - 5613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Bradley, C. Ward, S. J. Cheng, D. L. Alexander, S. Coller, G. H. Coombs, J. D. Dunn, D. J. Ferguson, S. J. Sanderson, J. M. Wastling, et al. Proteomic Analysis of Rhoptry Organelles Reveals Many Novel Constituents for Host-Parasite Interactions in Toxoplasma gondii J. Biol. Chem., October 7, 2005; 280(40): 34245 - 34258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Harris, J. L. Casey, A. M. Coley, R. Masciantonio, J. K. Sabo, D. W. Keizer, E. F. Lee, A. McMahon, R. S. Norton, R. F. Anders, et al. Binding Hot Spot for Invasion Inhibitory Molecules on Plasmodium falciparum Apical Membrane Antigen 1 Infect. Immun., October 1, 2005; 73(10): 6981 - 6989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mahajan, D. Jani, R. Chattopadhyay, R. Nagarkatti, H. Zheng, V. Majam, W. Weiss, S. Kumar, and D. Rathore Identification, Cloning, Expression, and Characterization of the Gene for Plasmodium knowlesi Surface Protein Containing an Altered Thrombospondin Repeat Domain Infect. Immun., September 1, 2005; 73(9): 5402 - 5409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stephens, F. R. Albano, S. Quin, B. J. Pascal, V. Harrison, B. Stockinger, D. Kioussis, H.-U. Weltzien, and J. Langhorne Malaria-specific transgenic CD4+ T cells protect immunodeficient mice from lethal infection and demonstrate requirement for a protective threshold of antibody production for parasite clearance Blood, September 1, 2005; 106(5): 1676 - 1684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Giersing, K. Miura, R. Shimp, J. Wang, H. Zhou, A. Orcutt, A. Stowers, A. Saul, L. H. Miller, C. Long, et al. Posttranslational Modification of Recombinant Plasmodium falciparum Apical Membrane Antigen 1: Impact on Functional Immune Responses to a Malaria Vaccine Candidate Infect. Immun., July 1, 2005; 73(7): 3963 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Winter, S. Kawai, M. Haeggstrom, O. Kaneko, A. von Euler, S.-i. Kawazu, D. Palm, V. Fernandez, and M. Wahlgren SURFIN is a polymorphic antigen expressed on Plasmodium falciparum merozoites and infected erythrocytes J. Exp. Med., June 6, 2005; 201(11): 1853 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Knuepfer, M. Rug, N. Klonis, L. Tilley, and A. F. Cowman Trafficking of the major virulence factor to the surface of transfected P falciparum-infected erythrocytes Blood, May 15, 2005; 105(10): 4078 - 4087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kato, D. C. G. Mayer, S. Singh, M. Reid, and L. H. Miller Domain III of Plasmodium falciparum apical membrane antigen 1 binds to the erythrocyte membrane protein Kx PNAS, April 12, 2005; 102(15): 5552 - 5557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Healer, T. Triglia, A. N. Hodder, A. W. Gemmill, and A. F. Cowman Functional Analysis of Plasmodium falciparum Apical Membrane Antigen 1 Utilizing Interspecies Domains Infect. Immun., April 1, 2005; 73(4): 2444 - 2451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cortes, M. Mellombo, R. Masciantonio, V. J. Murphy, J. C. Reeder, and R. F. Anders Allele Specificity of Naturally Acquired Antibody Responses against Plasmodium falciparum Apical Membrane Antigen 1 Infect. Immun., January 1, 2005; 73(1): 422 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Gaffar, A. P. Yatsuda, F. F. J. Franssen, and E. de Vries Erythrocyte Invasion by Babesia bovis Merozoites Is Inhibited by Polyclonal Antisera Directed against Peptides Derived from a Homologue of Plasmodium falciparum Apical Membrane Antigen 1 Infect. Immun., May 1, 2004; 72(5): 2947 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Silvie, J.-F. Franetich, S. Charrin, M. S. Mueller, A. Siau, M. Bodescot, E. Rubinstein, L. Hannoun, Y. Charoenvit, C. H. Kocken, et al. A Role for Apical Membrane Antigen 1 during Invasion of Hepatocytes by Plasmodium falciparum Sporozoites J. Biol. Chem., March 5, 2004; 279(10): 9490 - 9496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Mitchell, A. W. Thomas, G. Margos, A. R. Dluzewski, and L. H. Bannister Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells Infect. Immun., January 1, 2004; 72(1): 154 - 158. [Abstract] [Full Text] [PDF]
|
|
