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First published online 30 July 2003
doi: 10.1242/jcs.00685

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Interphase and monoastral-mitotic phenotypes of overexpressed MAP4 are modulated by free tubulin concentrations

¹ Department of Molecular Biology, University of Umea, S-901 87 Umea, Sweden
² Department of Medical Biosciences, University of Umea, S-901 87 Umea, Sweden

^* Author for correspondence (e-mail: martin.gullberg{at}molbiol.umu.se)

Accepted 27 May 2003

The microtubule-associated protein 4 (MAP4) has recently been shown to counteract destabilization of interphase microtubules caused by catastrophe promotion but not by tubulin sequestering. To address how MAP4 discriminates between destabilization of microtubules by these two mechanisms, we have evaluated the combined phenotypes of MAP4 coexpressed with Op18/stathmin family member derivatives with either catastrophe-promoting or sequestering activities. This approach relies on the finding that overexpression of MAP4 alone stabilizes microtubules during all phases of the cell cycle in human leukemia cells, and causes a potent mitotic block and a dramatic, previously unobserved, phenotype characterized by large monoastral spindles. Coexpression of either catastrophe-promoting or tubulin-sequestration-specific Op18 derivatives was found to modulate the activity of ectopic MAP4 during mitosis, but with differential functional outcome. Interestingly, the tubulin-sequestering derivative suppressed the monoastral mitotic phenotype of MAP4 (i.e. coexpression facilitated the formation of functional spindles). To evaluate whether this phenotypic suppression could be explained by tubulin-sequestration-dependent modulation of MAP4 activity, a plasma-membrane-targeted, tubulin-sequestering chimera was constructed to decrease the cytosolic free tubulin concentration substantially. This chimera likewise suppressed the monoastral phenotype caused by overexpression of MAP4, suggesting a direct downregulation of MAP4 activity by reduced free tubulin concentrations.

Key words: Microtubules, Tubulin, Microtubule-associated proteins, Recombinant fusion proteins, MAP4

This article has been cited by other articles:

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