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First published online 23 April 2003
doi: 10.1242/jcs.00439

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Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation

¹ Biology Department, University of Nevada, Reno, NV 89557, USA
² Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA

^* Author for correspondence (e-mail: mccracke{at}unr.edu)

Accepted 24 February 2003

In the eukaryotic cell, a protein quality control process termed endoplasmic reticulum-associated degradation (ERAD) rids the ER of aberrant proteins and unassembled components of protein complexes that fail to reach a transport-competent state. To identify novel genes required for ERAD, we devised a rapid immunoassay to screen yeast lacking uncharacterized open reading frames that were known targets of the unfolded protein response (UPR), a cellular response that is induced when aberrant proteins accumulate in the ER. Six genes required for the efficient degradation of the Z variant of the 1-proteinase inhibitor (A1PiZ), a known substrate for ERAD, were identified, and analysis of other ERAD substrates in the six A1PiZ-degradation-deficient (add) mutants suggested diverse requirements for the Add proteins in ERAD. Finally, we report on bioinformatic analyses of the new Add proteins, which will lead to testable models to elucidate their activities.

Key words: ERAD, Protein quality control, Degradation

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