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First published online 18 March 2003
doi: 10.1242/jcs.00392

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Transient association of the sarcoplasmic reticulum Ca²⁺ ATPase with the Na⁺/K⁺-ATPase and H⁺/K⁺-ATPase ß-subunits during its biogenesis in Xenopus oocytes

¹ Department of Biochemical Engineering and Science, Kyushu Institute of Technology Iizuka, Fukuoka 820-8502, Japan
² Department of Molecular Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan

^* Author for correspondence (e-mail: xenopus{at}med.uoeh-u.ac.jp)

Accepted 27 January 2003

We examined the effect of the ß-subunits of the Na⁺/K⁺ and H⁺/K⁺ ATPases on the biogenesis of the sarcoplasmic reticulum (SR) Ca²⁺ ATPase in Xenopus oocytes. Oocytes were simultaneously injected with cRNAs for both the SR Ca²⁺ ATPase and the ß-subunit of the Na⁺/K⁺ or the H⁺/K⁺ ATPase. Immunoprecipitation with antiserum specific for the ß-subunit of the Na⁺/K⁺ or the H⁺/K⁺ ATPase yielded not only the respective ß-subunit but also the SR Ca²⁺ ATPase, indicating that the SR Ca²⁺ ATPase was associated with the ß-subunits of the Na⁺/K⁺ and the H⁺/K⁺ ATPases. Pulse-chase experiments revealed that the complex between the SR Ca²⁺ ATPase and the ß-subunit of the Na⁺/K⁺ ATPase was formed transiently and dissociated during the course of maturation. This is the first report that demonstrates the association of the SR Ca²⁺ ATPase with the ß-subunit of the Na⁺/K⁺ and H⁺/K⁺ ATPases.

Key words: Ca²⁺ ATPase, Na⁺/K⁺ ATPase, ATPase ß-subunit, Chaperone

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