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Journal of Cell Science 115, 1769-1775 (2002)
© 2002 The Company of Biologists Limited

Research Article

Nuclear localization of phosphatidylinositol 4-kinase ß

Petra de Graaf, Elsa E. Klapisz, Thomas K. F. Schulz, Alfons F. M. Cremers, Arie J. Verkleij and Paul M. P. van Bergen en Henegouwen*

Molecular Cell Biology, Institute of Biomembranes, Universiteit Utrecht, The Netherlands

* Author for correspondence (e-mail: bergenp{at}bio.uu.nl )

Accepted 18 January 2002

Whereas most phosphatidylinositol 4-kinase (PtdIns 4-kinase) activity is localized in the cytoplasm, PtdIns 4-kinase activity has also been detected in membranedepleted nuclei of rat liver and mouse NIH 3T3 cells. Here we have characterized the PtdIns 4-kinase that is present in nuclei from NIH 3T3 cells. Both type II and type III PtdIns 4-kinase activity were observed in the detergent-insoluble fraction of NIH 3T3 cells. Dissection of this fraction into cytoplasmic actin filaments and nuclear lamina-pore complexes revealed that the actin filament fraction contains solely type II PtdIns 4-kinase, whereas lamina-pore complexes contain type III PtdIns 4-kinase activity. Using specific antibodies, the nuclear PtdIns 4-kinase was identified as PtdIns 4-kinase ß. Inhibition of nuclear export by leptomycin B resulted in an accumulation of PtdIns 4-kinase ß in the nucleus. These data demonstrate that PtdIns 4-kinase ß is present in the nuclei of NIH 3T3 fibroblasts, suggesting a specific function for this kinase in nuclear processes.

Key words: Phosphatidylinositol 4-kinase, Detergent insoluble fraction, Actin filaments, Nucleus, NIH 3T3 fibroblasts




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© The Company of Biologists Ltd 2002