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Research Article |
1 Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund, London, WC2A
3PX, UK
2 Sackler Institute for Muscular Skeletal Research, Department of Medicine,
University College London, 5 University Street, London, WC1E 6JJ, UK
* Author for correspondence (e-mail: hogg{at}icrf.icnet.uk
Accepted 4 December 2001
Integrin activity on cells such as T lymphocytes is tightly controlled.
Here we demonstrate a key role for lipid rafts in regulating integrin
function. Without stimulation integrin LFA-1 is excluded from lipid rafts, but
following activation LFA-1 is mobilised to the lipid raft compartment. An
LFA-1 construct from which the I domain has been deleted mimics activated
integrin and is constitutively found in lipid rafts. This correlation between
integrin activation and raft localisation extends to a second integrin,
4ß1, and the clustering of 4ß1 is also raft dependent.
Both LFA-1 and 4ß1-mediated adhesion is dependent upon intact
lipid rafts providing proof of the functional relevance of the lipid raft
localisation. Finally we find that non-raft integrins are excluded from the
rafts by cytoskeletal constraints. The presence of integrin in lipid rafts
under stimulating conditions that activate these receptors strongly indicates
that the rafts have a key role in positively regulating integrin activity.
Key words: Integrin, Lipid rafts, Cytoskeleton
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|
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