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doi: 10.1242/10.1242/jcs.00066

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Journal of Cell Science 115, 3829-3835 (2002)
Copyright © 2002 The Company of Biologists Limited
doi: 10.1242/jcs.00066

Research Article

Spatial organisation and behaviour of the parental chromosome sets in the nuclei of Saccharomyces cerevisiae x S. paradoxus hybrids

Alexander Lorenz1, Jörg Fuchs1, Edgar Trelles-Sticken2, Harry Scherthan2 and Josef Loidl1,*

1 Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria
2 Max-Planck-Institute for Molecular Genetics, Ihnestraße 73, D-14195 Berlin, Germany

* Author for correspondence (e-mail: josef.loidl{at}univie.ac.at)

Accepted 24 July 2002

We demonstrate that the genomes of Saccharomyces cerevisiae and S. paradoxus are sufficiently divergent to allow their differential labeling by genomic in situ hybridisation (GISH). The cytological discrimination of the genomes allowed us to study the merging of the two genomes during hybrid mating. GISH revealed that in hybrid nuclei the two genomes are intermixed. In hybrid meiosis, extensive intraspectific nonhomologous pairing takes place. GISH on chromosome addition and substitution strains (with chromosomes of S. paradoxus added to or replacing the homoeologous chromosome of an otherwise S. cerevisiae background) was used to delineate individual chromosomes at interphase and to examine various aspects of chromosome structure and arrangement.

Key words: Yeast, Hybrid, Zygote, Nuclear architecture, FISH, Interphase, Meiosis, Chromosome




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© The Company of Biologists Ltd 2002