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doi: 10.1242/10.1242/jcs.00054

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Reorganization and polarization of the meiotic bouquet-stage cell can be uncoupled from telomere clustering

¹ Department of Plant and Microbial Biology, University of California — Berkeley, Berkeley, CA 94720-3200, USA
² Department of Molecular and Cell Biology, University of California — Berkeley, Berkeley, CA 94720-3200, USA

^* Author for correspondence (e-mail: zcande{at}uclink4.berkeley.edu)

Accepted 14 July 2002

Striking cellular reorganizations mark homologous pairing during meiotic prophase. We address the interdependence of chromosomal and cellular polarization during meiotic telomere clustering, the defining feature of the bouquet stage, by examining nuclear positioning and microtubule and nuclear pore reorganization. Polarization of meiotic cellular architecture was coincident with telomere clustering: microtubules were focused on the nuclear surface opposite the telomere cluster, the nucleus was positioned eccentrically in the cell such that the telomeres faced the direction of nuclear displacement and nuclear pores were clustered in a single region of the nuclear surface opposite the telomeres. Treatment of pre-bouquet stage cells with colchicine inhibited telomere clustering. Asymmetric nuclear positioning and nuclear pore clustering were normal in the presence of unclustered telomeres resulting from colchicine treatment. Nuclear pores were positioned normally with respect to the cell cortex in the absence of telomere clustering, indicating that telomere positioning is not required for polarization. This work provides evidence of meiotic cell polarization and suggests that telomeres may be positioned relative to an asymmetry present in the cell at the time of bouquet formation.

Key words: Bouquet, Telomere clustering, Meiosis, Nuclear pore clustering, Cell polarity

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Telomere clustering

JCS 2002 115: 1904. [Full Text]  

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				P. M. Carlton, C. R. Cowan, and W. Z. Cande Directed Motion of Telomeres in the Formation of the Meiotic Bouquet Revealed by Time Course and Simulation Analysis Mol. Biol. Cell, July 1, 2003; 14(7): 2832 - 2843. [Abstract] [Full Text] [PDF]

				C. R. Cowan and W. Z. Cande Meiotic telomere clustering is inhibited by colchicine but does not require cytoplasmic microtubules J. Cell Sci., January 10, 2002; 115(19): 3747 - 3756. [Abstract] [Full Text] [PDF]