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Journal of Cell Science 115, 2293-2302 (2002)
© 2002 The Company of Biologists Limited

Research Article

A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo

Susanne Kaitna1, Heinke Schnabel2, Ralf Schnabel2, Anthony A. Hyman3 and Michael Glotzer1,*

1 Research Institute for Molecular Pathology, Dr Bohr-Gasse 7, A-1030 Vienna, Austria
2 Technical University Braunschweig, D-38106, Braunschweig, Germany
3 Max Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany

* Author for correspondence (e-mail: mglotzer{at}nt.imp.univie.ac.at )

Accepted 18 March 2002

In the early Caenorhabditis elegans embryo, establishment of cell polarity and cytokinesis are both dependent upon reorganization of the actin cytoskeleton. Mutations in the cyk-3 gene cause maternal effect embryonic lethality. Embryos produced by homozygous cyk-3 mutant animals become multinucleate. We have further analyzed the cyk-3 mutant phenotype and have found that cyk-3 mutant embryos fail to properly polarize the actin cytoskeleton and fail to segregate germline determinants. In addition, they fail to assemble an intact cleavage furrow. However, we have found that cyk-3 mutant embryos are intrinsically defective in osmotic regulation and that the cytokinesis defects can be partially rescued by providing osmotic support. The cyk-3 gene has been identified and found to encode a ubiquitin C-terminal hydrolase that is active against model substrates. These data indicate that the deubiquitination of certain substrates by CYK-3 is crucial for cellular osmoregulation. Defects in osmoregulation appear to indirectly affect actin-dependent processes.

Key words: Polarity, Cytokinesis, Cleavage furrow, Protein degradation, Endocytosis




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© The Company of Biologists Ltd 2002