|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 9 1699-1708, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
MD Haskell, AL Nickles, JM Agati, L Su, BD Dukes and SJ Parsons
Department of Microbiology and Cancer Center, Box 800734, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA. sap@virginia.edu
p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 participates in actin cytoskeleton rearrangements that occur following transformation by v-Src or stimulation by growth factors, and that tyrosine phosphorylation of p190 by Src influences these processes. The current study was performed to establish whether p190RhoGAP directly participates in epidermal growth factor-induced actin stress fiber disassembly and how c-Src is involved in this process. Our results support a model in which the p190 MD negatively regulates the activity of the GAP domain and that c-Src phosphorylation of Y1105 is necessary, but insufficient on its own, for actin stress fiber disassembly.
This article has been cited by other articles:
|
|
 |
|
  W. Jiang, M. Betson, R. Mulloy, R. Foster, M. Levay, E. Ligeti, and J. Settleman p190A RhoGAP Is a Glycogen Synthase Kinase-3-{beta} Substrate Required for Polarized Cell Migration J. Biol. Chem., July 25, 2008; 283(30): 20978 - 20988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Fang, R. C. Ireton, G. Zhuang, T. Takahashi, A. Reynolds, and J. Chen Overexpression of EPHA2 receptor destabilizes adherens junctions via a RhoA-dependent mechanism J. Cell Sci., February 1, 2008; 121(3): 358 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Playford, P. D. Lyons, S. K. Sastry, and M. D. Schaller Identification of a Filamin Docking Site on PTP-PEST J. Biol. Chem., November 10, 2006; 281(45): 34104 - 34112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Park, S. M. Ward, J. S. Desgrosellier, S. P. Georgescu, A. G. Papageorge, X. Zhuang, J. V. Barnett, and J. B. Galper Transforming Growth Factor beta Regulates the Expression of the M2 Muscarinic Receptor in Atrial Myocytes via an Effect on RhoA and p190RhoGAP J. Biol. Chem., July 21, 2006; 281(29): 19995 - 20002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurokawa and M. Matsuda Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling Mol. Biol. Cell, September 1, 2005; 16(9): 4294 - 4303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Burgstaller and M. Gimona Actin cytoskeleton remodelling via local inhibition of contractility at discrete microdomains J. Cell Sci., January 15, 2004; 117(2): 223 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Su, J. M. Agati, and S. J. Parsons p190RhoGAP is cell cycle regulated and affects cytokinesis J. Cell Biol., November 10, 2003; 163(3): 571 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. SOMLYO and A. V. SOMLYO Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II: Modulated by G Proteins, Kinases, and Myosin Phosphatase Physiol Rev, October 1, 2003; 83(4): 1325 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. GRATZINGER, S. CANOSA, B. ENGELHARDT, and J. A. MADRI Platelet endothelial cell adhesion molecule-1 modulates endothelial cell motility through the small G-protein Rho FASEB J, August 1, 2003; 17(11): 1458 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shiota, T. Tanihiro, Y. Nakagawa, N. Aoki, N. Ishida, K. Miyazaki, A. Ullrich, and H. Miyazaki Protein Tyrosine Phosphatase PTP20 Induces Actin Cytoskeleton Reorganization by Dephosphorylating p190 RhoGAP in Rat Ovarian Granulosa Cells Stimulated with Follicle-Stimulating Hormone Mol. Endocrinol., April 1, 2003; 17(4): 534 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Wolf, N. Draghi, X. Liang, C. Dai, L. Uhrbom, C. Eklof, B. Westermark, E. C. Holland, and M. D. Resh p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human Chromosome 19q13.3 Genes & Dev., February 15, 2003; 17(4): 476 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Moon, H. Zang, and Y. Zheng Characterization of a Brain-specific Rho GTPase-activating Protein, p200RhoGAP J. Biol. Chem., January 31, 2003; 278(6): 4151 - 4159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Brandt, M. Gimona, M. Hillmann, H. Haller, and H. Mischak Protein Kinase C Induces Actin Reorganization via a Src- and Rho-dependent Pathway J. Biol. Chem., May 31, 2002; 277(23): 20903 - 20910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jenna, N. K. Hussain, E. I. Danek, I. Triki, S. Wasiak, P. S. McPherson, and N. Lamarche-Vane The Activity of the GTPase-activating Protein CdGAP Is Regulated by the Endocytic Protein Intersectin J. Biol. Chem., February 15, 2002; 277(8): 6366 - 6373. [Abstract] [Full Text] [PDF]
|
|
