spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Recalcati, S.
Right arrow Articles by Kuhn, L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Recalcati, S.
Right arrow Articles by Kuhn, L. C.

Journal of Cell Science, Vol 114, Issue 9 1625-1629, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Peroxisomal targeting of mammalian hydroxyacid oxidase 1 requires the C-terminal tripeptide SKI

S Recalcati, E Menotti and LC Kuhn
Swiss Institute for Experimental Cancer Research, Ch. des Boveresses, CH-1066 Epalinges, s/Lausanne, Switzerland. lukas.kuehn@isrec.unil.ch

Peroxisomal proteins are post-translationally imported into peroxisomes after recognition by specific receptors. The best-defined peroxisomal targeting signal is a C-terminal tripeptide SKL. Different functional variants of this tripeptide have been defined, but mutants with a SKI sequence were recognized as being inefficiently targeted to peroxisomes. Recently, we have cloned a cDNA for the mouse hydroxyacid oxidase 1 (Hao1), a protein that seems to be localized in peroxisomes. Interestingly, the mouse Hao1 sequence comprises a C-terminal SKI tripeptide. We have analyzed the subcellular localization of Hao1 and tested whether its SKI sequence acts as a targeting signal. Ltk(-) and Cos-7 cells were transfected with vectors expressing a fusion protein of green fluorescence protein and Hao1, as well as mutants thereof. Targeting to peroxisomes of the fusion protein with the wild-type SKI sequence was highly selective and as complete as with the peroxisome-specific SKL sequence. By contrast, targeting was lost in a mutant with the sequence CKM. The data show that mammalian Hao1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Murillas, K. S. Simms, S. Hatakeyama, A. M. Weissman, and M. R. Kuehn
Identification of Developmentally Expressed Proteins That Functionally Interact with Nedd4 Ubiquitin Ligase
J. Biol. Chem., January 18, 2002; 277(4): 2897 - 2907.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001