|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 2 435-443, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
F Vidal, P Lopez, LA Lopez-Fernandez, F Ranc, JC Scimeca, F Cuzin and M Rassoulzadegan
Unite 470 de l'Institut National de la Sante et de la Recherche Medicale and Unite Mixte CNRS-Universite 6549, Universite de Nice, France.
Analysis of complex signalisation networks involving distinct cell types is required to understand most developmental processes. Differentiation of male germ cells in adult mammals involves such a cross-talk between Sertoli cells, the somatic component which supports and controls germinal differentiation, and germ cells at their successive maturation stages. We developed a gene trapping strategy to identify genes, which, in Sertoli cells, are either up- or down-regulated by signals emitted by the germinal component. A library of approximately 2,000 clones was constituted from colonies independently selected from the Sertoli line 15P-1 by growth in drug-containing medium after random integration of a promoter-less (beta)geo transgene (neo(r)-lacZ fusion), which will be expressed as a fusion transcript from a 'trapped' cellular promoter, different in each clone. A first screen conducted on 700 events identified six clones in which beta-galactosidase activity was increased and one in which it was repressed upon addition of germ cells. The targeted loci were identified by cloning and sequencing the genomic region 5' of the insert. One of them was identified as the gene encoding Fra1, a component of the AP1 transcription regulatory complex. Accumulation of Fra1 mRNA was induced, both in 15P-1 and in freshly explanted Sertoli cells, by addition of either round spermatids or nerve growth factor (NGF). The effect of NGF was mediated by the TrkA receptor and the ERK1-ERK2 kinase kinase pathway. Fos and Fra1 transcription were induced within the first hour after addition of the neurotrophin, but, unlike what is observed after serum induction in the same cells, a second wave of transcription of Fra1, but not of Fos, started 16 hours later and peaked at higher levels at about 20 hours. These results suggest that AP1 activation may be an important relay in the Sertoli-germ cell cross-talk, and validate the gene trapping approach as a tool for the identification of target genes in cell culture systems.
This article has been cited by other articles:
|
|
 |
|
  S. Moritz, S. Lehmann, A. Faissner, and A. von Holst An Induction Gene Trap Screen in Neural Stem Cells Reveals an Instructive Function of the Niche and Identifies the Splicing Regulator Sam68 as a Tenascin-C-Regulated Target Gene Stem Cells, September 1, 2008; 26(9): 2321 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P J O'Shaughnessy, L Hu, and P J Baker Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells Reproduction, June 1, 2008; 135(6): 839 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Olive, N. Wagner, S. Chan, P. Kastner, C. Vannetti, F. Cuzin, and M. Rassoulzadegan PU.1 (Sfpi1), a pleiotropic regulator expressed from the first embryonic stages with a crucial function in germinal progenitors Development, November 1, 2007; 134(21): 3815 - 3825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-S. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, H. Kogo, and T. Hara Spermatogonial Cell-Mediated Activation of an I{kappa}B{zeta}-Independent Nuclear Factor-{kappa}B Pathway in Sertoli Cells Induces Transcription of the Lipocalin-2 Gene Mol. Endocrinol., April 1, 2006; 20(4): 904 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gillot, C. Jehl-Pietri, P. Gounon, S. Luquet, M. Rassoulzadegan, P. Grimaldi, and F. Vidal Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells J. Cell Sci., July 15, 2005; 118(14): 3027 - 3035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hong, I. Choi, J.-M. Woo, J. Oh, T. Kim, E. Choi, T.-W. Kim, Y.-K. Jung, D. H. Kim, C.-H. Sun, et al. Identification and Integrative Analysis of 28 Novel Genes Specifically Expressed and Developmentally Regulated in Murine Spermatogenic Cells J. Biol. Chem., March 4, 2005; 280(9): 7685 - 7693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Shima, D. J. McLean, J. R. McCarrey, and M. D. Griswold The Murine Testicular Transcriptome: Characterizing Gene Expression in the Testis During the Progression of Spermatogenesis Biol Reprod, July 1, 2004; 71(1): 319 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Maier, S. Medrano, S. B. Sleight, P. E. Visconti, and H. Scrable Developmental Association of the Synaptic Activity-Regulated Protein Arc with the Mouse Acrosomal Organelle and the Sperm Tail Biol Reprod, January 1, 2003; 68(1): 67 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lopez, F. Vidal, L. Martin, L. A. Lopez-Fernandez, J.-F. Rual, B. S. Rosen, F. Cuzin, and M. Rassoulzadegan Gene Control in Germinal Differentiation: Rnf6, a Transcription Regulatory Protein in the Mouse Sertoli Cell Mol. Cell. Biol., May 15, 2002; 22(10): 3488 - 3496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Eddy Male Germ Cell Gene Expression Recent Prog. Horm. Res., January 1, 2002; 57(1): 103 - 128. [Abstract] [Full Text] [PDF]
|
|
