spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ersfeld, K.
Right arrow Articles by Gull, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ersfeld, K.
Right arrow Articles by Gull, K.

Journal of Cell Science, Vol 114, Issue 1 141-148, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Targeting of cytoskeletal proteins to the flagellum of Trypanosoma brucei

K Ersfeld and K Gull
School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK. k.ersfeld@man.ac.uk

The eukaryotic flagellum represents one of the most complex macromolecular structures found in any organism and contains more than 250 proteins. Due to the relative ease of genetic manipulation the flagellum of Trypanosoma brucei has emerged as an accessible model system to study the morphogenesis and dynamics of this organelle. We have recently started to characterise the mechanisms by which components of the cytoskeletal fraction of the flagellum, such as the axoneme, the paraflagellar rod and the flagellar attachment zone, are targeted by proteins synthesised in the cytoplasm and assembled. Here, we present the identification of a novel actin-related protein as a component of the axoneme. We show that this protein shares the tripeptid motif histidine-leucine-alanine (HLA) with one of the major proteins of the paraflagellar rod, PFRA. Building on previous work from this lab which showed that a deletion comprising this motif abolished targeting of PFRA to the flagellum we demonstrate in this study that the deletion of the tripeptid motif is sufficient to achieve mistargeting both of the PFRA and the actin-related protein. We propose that this motif represents an essential part of a flagellar targeting machinery in trypanosomes and possibly in other flagellated organisms.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
L. Geng, D. Okuhara, Z. Yu, X. Tian, Y. Cai, S. Shibazaki, and S. Somlo
Polycystin-2 traffics to cilia independently of polycystin-1 by using an N-terminal RVxP motif
J. Cell Sci., April 1, 2006; 119(7): 1383 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. R. Matthews
The developmental cell biology of Trypanosoma brucei
J. Cell Sci., January 15, 2005; 118(2): 283 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. J. Pullen, M. L. Ginger, S. J. Gaskell, and K. Gull
Protein Targeting of an Unusual, Evolutionarily Conserved Adenylate Kinase to a Eukaryotic Flagellum
Mol. Biol. Cell, July 1, 2004; 15(7): 3257 - 3265.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Escalier
New Insights into the Assembly of the Periaxonemal Structures in Mammalian Spermatozoa
Biol Reprod, August 1, 2003; 69(2): 373 - 378.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Pan and W. J. Snell
Kinesin II and regulated intraflagellar transport of Chlamydomonas aurora protein kinase
J. Cell Sci., June 1, 2003; 116(11): 2179 - 2186.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001