|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 8 1169-1179, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
A Moustakas and C Stournaras
Department of Biochemistry, Division of Basic Sciences, School of Medicine, University of Crete, Heraklion, Greece.
The actin cytoskeleton undergoes architectural changes during the processes of cell transformation and tumourigenesis. Transforming growth factors beta arrest cell cycle progression, regulate differentiation and modulate the onset of oncogenesis and tumourigenesis. Here, we investigated the direct role of transforming growth factor beta-1 in altering the transformed phenotype and regulating the actin organisation of oncogenic fibroblasts that constitutively or inducibly express the H-ras oncogene. Following transforming growth factor beta-1 treatment, these transformed fibroblasts undergo a dramatic morphological alteration that includes a discrete reorganisation of their actin cytoskeleton and focal adhesions. Quantitative biochemical analysis demonstrated that transforming growth factor beta-1 potently induced polymerisation of globular to filamentous actin, thus corroborating the morphological analysis. The effect of transforming growth factor beta-1 on the cytoskeleton correlates with the ability of this cytokine to suppress anchorage-independent growth of the transformed fibroblasts. Furthermore, transforming growth factor beta-1 upregulates considerably the levels of the RhoB small GTPase and less the RhoA levels. Finally, The beta GTPase inhibitor, C3 exotransferase, blocks the ability of TGF-beta1 to induce cytoskeletal reorganisation. These findings indicate that transforming growth factor beta can regulate cell morphology and growth in a concerted manner possibly via mechanisms that control the actin cytoskeleton.
This article has been cited by other articles:
|
|
 |
|
  J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, et al. Optical Deformability as an Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence Biophys. J., May 1, 2005; 88(5): 3689 - 3698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Clement, B. Hinz, V. Dugina, G. Gabbiani, and C. Chaponnier The N-terminal Ac-EEED sequence plays a role in {alpha}-smooth-muscle actin incorporation into stress fibers J. Cell Sci., April 1, 2005; 118(7): 1395 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vardouli, A. Moustakas, and C. Stournaras LIM-kinase 2 and Cofilin Phosphorylation Mediate Actin Cytoskeleton Reorganization Induced by Transforming Growth Factor-{beta} J. Biol. Chem., March 25, 2005; 280(12): 11448 - 11457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Bakin, A. Safina, C. Rinehart, C. Daroqui, H. Darbary, and D. M. Helfman A Critical Role of Tropomyosins in TGF-{beta} Regulation of the Actin Cytoskeleton and Cell Motility in Epithelial Cells Mol. Biol. Cell, October 1, 2004; 15(10): 4682 - 4694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Scaffidi, N. Petrovic, Y. P. Moodley, M. Fogel-Petrovic, K. M. Kroeger, R. M. Seeber, K. A. Eidne, P. J. Thompson, and D. A. Knight {alpha}v{beta}3 Integrin Interacts with the Transforming Growth Factor {beta} (TGF{beta}) Type II Receptor to Potentiate the Proliferative Effects of TGF{beta}1 in Living Human Lung Fibroblasts J. Biol. Chem., September 3, 2004; 279(36): 37726 - 37733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Chen, S. Zhuang, T. H. Nguyen, G. R. Boss, and R. B. Pilz Oncogenic Ras Leads to Rho Activation by Activating the Mitogen-activated Protein Kinase Pathway and Decreasing Rho-GTPase-activating Protein Activity J. Biol. Chem., January 24, 2003; 278(5): 2807 - 2818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W J Lemon, H Bernert, H Sun, Y Wang, and M You Identification of candidate lung cancer susceptibility genes in mouse using oligonucleotide arrays J. Med. Genet., September 1, 2002; 39(9): 644 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Edlund, M. Landstrom, C.-H. Heldin, and P. Aspenstrom Transforming Growth Factor-beta -induced Mobilization of Actin Cytoskeleton Requires Signaling by Small GTPases Cdc42 and RhoA Mol. Biol. Cell, March 1, 2002; 13(3): 902 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-X. Liu, N. Rane, J.-P. Liu, and G. C. Prendergast RhoB Is Dispensable for Mouse Development, but It Modifies Susceptibility to Tumor Formation as Well as Cell Adhesion and Growth Factor Signaling in Transformed Cells Mol. Cell. Biol., October 15, 2001; 21(20): 6906 - 6912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. HEUSINGER-RIBEIRO, M. EBERLEIN, N. A. WAHAB, and M. GOPPELT-STRUEBE Expression of Connective Tissue Growth Factor in Human Renal Fibroblasts: Regulatory Roles of RhoA and cAMP J. Am. Soc. Nephrol., September 1, 2001; 12(9): 1853 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dermitzaki, A. Gravanis, M. Venihaki, C. Stournaras, and A. N. Margioris Opioids Suppress Basal and Nicotine-Induced Catecholamine Secretion Via a Stabilizing Effect on Actin Filaments Endocrinology, May 1, 2001; 142(5): 2022 - 2031. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Reuveni, T. Geiger, B. Geiger, and A. Levitzki Reversal of the Ras-induced Transformed Phenotype by HR12, a Novel Ras Farnesylation Inhibitor, Is Mediated by the Mek/Erk Pathway J. Cell Biol., December 11, 2000; 151(6): 1179 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Piek, A Moustakas, A Kurisaki, C. Heldin, and P ten Dijke TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells J. Cell Sci., January 12, 1999; 112(24): 4557 - 4568. [Abstract] [PDF]
|
|
|
|
|
|
H. Jornvall, A. Blokzijl, P. t. Dijke, and C. F. Ibanez The Orphan Receptor Serine/Threonine Kinase ALK7 Signals Arrest of Proliferation and Morphological Differentiation in a Neuronal Cell Line J. Biol. Chem., February 9, 2001; 276(7): 5140 - 5146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shen, J. Li, P. P.-c. Hu, D. Waddell, J. Zhang, and X.-F. Wang The Activity of Guanine Exchange Factor NET1 Is Essential for Transforming Growth Factor-beta -mediated Stress Fiber Formation J. Biol. Chem., April 27, 2001; 276(18): 15362 - 15368. [Abstract] [Full Text] [PDF]
|
|
