|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 3 349-359, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
J Heeren, W Weber and U Beisiegel
Medical Clinic, University Hospital Eppendorf, Hamburg, Germany.
The current study was performed to investigate the intracellular fate of triglyceride-rich lipoproteins. Triglyceride-rich lipoproteins are responsible for the delivery of lipids to various tissues, however, their intracellular pathway has not yet been elucidated. Here radiolabeled triglyceride-rich lipoproteins, associated with lipoprotein lipase, were used for the quantitative evaluation of the intracellular metabolism. Pulse chase experiments showed that after 90 minutes approximately 60% of the labeled protein, mainly apoproteins E and C, was released intact into the medium, where it re-associates with lipoproteins. Apoprotein B, in contrast, was degraded, following the same pathway as the apoprotein B from low density lipoproteins. In kinetic experiments uptake and intracellular fate of triglyceride-rich lipoproteins was compared to that of transferrin and low density lipoproteins. These experiments revealed that apoproteins were retained inside the cell much longer than transferrin, and unlike low density lipoproteins were not degraded. Using immunofluorescence it was shown that apoprotein E and lipoprotein lipase follow a distinct route from the sorting compartment to the surface, which is clearly distinguishable from the perinuclear transferrin recycling compartment. In contrast, the fluorescence labeled lipids were delivered to lysosomal compartments. The data presented here show that surface proteins of triglyceride-rich lipoproteins, such as apoproteins E and C and lipoprotein lipase follow a recycling pathway, whereas lipids and high molecular mass core proteins are degraded.
This article has been cited by other articles:
|
|
 |
|
  A. Kontush and M. J. Chapman Functionally Defective High-Density Lipoprotein: A New Therapeutic Target at the Crossroads of Dyslipidemia, Inflammation, and Atherosclerosis Pharmacol. Rev., September 1, 2006; 58(3): 342 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Sun, E. R. M. Eckhardt, S. Shetty, D. R. van der Westhuyzen, and N. R. Webb Quantitative analysis of SR-BI-dependent HDL retroendocytosis in hepatocytes and fibroblasts J. Lipid Res., August 1, 2006; 47(8): 1700 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heeren, U. Beisiegel, and T. Grewal Apolipoprotein E Recycling: Implications for Dyslipidemia and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 442 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Hasty, M. R. Plummer, K. H. Weisgraber, M. F. Linton, S. Fazio, and L. L. Swift The recycling of apolipoprotein E in macrophages: influence of HDL and apolipoprotein A-I J. Lipid Res., July 1, 2005; 46(7): 1433 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Van Hoof, K. W. Rodenburg, and D. J. Van der Horst Intracellular fate of LDL receptor family members depends on the cooperation between their ligand-binding and EGF domains J. Cell Sci., March 15, 2005; 118(6): 1309 - 1320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heeren, T. Grewal, A. Laatsch, N. Becker, F. Rinninger, K.-A. Rye, and U. Beisiegel Impaired Recycling of Apolipoprotein E4 Is Associated with Intracellular Cholesterol Accumulation J. Biol. Chem., December 31, 2004; 279(53): 55483 - 55492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Farkas, K. H. Weisgraber, V. L. Shepherd, M. F. Linton, S. Fazio, and L. L. Swift The recycling of apolipoprotein E and its amino-terminal 22 kDa fragment: evidence for multiple redundant pathways J. Lipid Res., August 1, 2004; 45(8): 1546 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Brundert, J. Heeren, H. Greten, and F. Rinninger Hepatic lipase mediates an increase in selective uptake of HDL-associated cholesteryl esters by cells in culture independent from SR-BI J. Lipid Res., May 1, 2003; 44(5): 1020 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Heeren, T. Grewal, A. Laatsch, D. Rottke, F. Rinninger, C. Enrich, and U. Beisiegel Recycling of Apoprotein E Is Associated with Cholesterol Efflux and High Density Lipoprotein Internalization J. Biol. Chem., April 11, 2003; 278(16): 14370 - 14378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Farkas, L. L. Swift, A. H. Hasty, M. F. Linton, and S. Fazio The Recycling of Apolipoprotein E in Primary Cultures of Mouse Hepatocytes. EVIDENCE FOR A PHYSIOLOGIC CONNECTION TO HIGH DENSITY LIPOPROTEIN METABOLISM J. Biol. Chem., March 7, 2003; 278(11): 9412 - 9417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Van Hoof, K. W. Rodenburg, and D. J. Van der Horst Insect lipoprotein follows a transferrin-like recycling pathway that is mediated by the insect LDL receptor homologue J. Cell Sci., January 11, 2002; 115(21): 4001 - 4012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Heeren, T. Grewal, S. Jackle, and U. Beisiegel Recycling of Apolipoprotein E and Lipoprotein Lipase through Endosomal Compartments in Vivo J. Biol. Chem., November 2, 2001; 276(45): 42333 - 42338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Santamarina-Fojo, A. T. Remaley, E. B. Neufeld, and H. B. Brewer Jr. Regulation and intracellular trafficking of the ABCA1 transporter J. Lipid Res., September 1, 2001; 42(9): 1339 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. VON ECKARDSTEIN, C. LANGER, T. ENGEL, I. SCHAUKAL, A. CIGNARELLA, J. REINHARDT, S. LORKOWSKI, Z. LI, X. ZHOU, P. CULLEN, et al. ATP binding cassette transporter ABCA1 modulates the secretion of apolipoprotein E from human monocyte-derived macrophages FASEB J, July 1, 2001; 15(9): 1555 - 1561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. von Eckardstein, J.-R. Nofer, and G. Assmann High Density Lipoproteins and Arteriosclerosis : Role of Cholesterol Efflux and Reverse Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., January 1, 2001; 21(1): 13 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. N. Rensen, M. C. Jong, L. C. van Vark, H. van der Boom, W. L. Hendriks, T. J. C. van Berkel, E. A. L. Biessen, and L. M. Havekes Apolipoprotein E Is Resistant to Intracellular Degradation in Vitro and in Vivo. EVIDENCE FOR RETROENDOCYTOSIS J. Biol. Chem., March 17, 2000; 275(12): 8564 - 8571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takahashi and J. D. Smith Cholesterol efflux to apolipoprotein AI involves endocytosis and resecretion in a calcium-dependent pathway PNAS, September 28, 1999; 96(20): 11358 - 11363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Swift, M. H. Farkas, A. S. Major, K. Valyi-Nagy, M. F. Linton, and S. Fazio A Recycling Pathway for Resecretion of Internalized Apolipoprotein E in Liver Cells J. Biol. Chem., June 15, 2001; 276(25): 22965 - 22970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Neufeld, A. T. Remaley, S. J. Demosky, J. A. Stonik, A. M. Cooney, M. Comly, N. K. Dwyer, M. Zhang, J. Blanchette-Mackie, S. Santamarina-Fojo, et al. Cellular Localization and Trafficking of the Human ABCA1 Transporter J. Biol. Chem., July 13, 2001; 276(29): 27584 - 27590. [Abstract] [Full Text] [PDF]
|
|
