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Journal of Cell Science, Vol 112, Issue 21 3691-3702, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
WL Severt, TU Biber, X Wu, NB Hecht, RJ DeLorenzo and ER Jakoi
Department of Physiology, Medical College of Virginia/Virginia Commonwealth University, Richmond, VA 23298, USA.
Ribonucleoprotein particles (RNPs) are thought to be key players in somato-dendritic sorting of mRNAs in CNS neurons and are implicated in activity-directed neuronal remodeling. Here, we use reporter constructs and gel mobility shift assays to show that the testis brain RNA-binding protein (TB-RBP) associates with mRNPs in a sequence (Y element) dependent manner. Using antisense oligonucleotides (anti-ODN), we demonstrate that blocking the TB-RBP Y element binding site disrupts and mis-localizes mRNPs containing (alpha)-calmodulin dependent kinase II (alpha)-CAMKII) and ligatin mRNAs. In addition, we show that suppression of kinesin heavy chain motor protein alters only the localization of (alpha)-CAMKII mRNA. Thus, differential sorting of mRNAs involves multiple mRNPs and selective motor proteins permitting localized mRNAs to utilize common mechanisms for shared steps.
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