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Journal of Cell Science, Vol 112, Issue 21 3619-3626, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
K Gonda, M Katoh, K Hanyu, Y Watanabe and O Numata
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Tetrahymena p85 differs in mobility in two-dimensional SDS-polyacrylamide gel electrophoresis between wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cell extracts, and is localized to the presumptive division plane before the formation of the division furrow. The p85 contained three identical sequences which show homology to the calmodulin binding site of Ca(2+)/calmodulin dependent protein kinase Type II in Saccharomyces cerevisiae. We found the p85 directly interacts with Tetrahymena calmodulin in a Ca(2+)-dependent manner, using a co-sedimentation assay. We next examined the localization of p85 and calmodulin during cytokinesis using indirect immunofluorescence. The results showed that both proteins colocalize in the division furrow. This is the first observation that calmodulin is localized in the division furrow. Moreover, the direct interaction between p85 and Ca(2+)/calmodulin was inhibited by Ca(2+)/calmodulin inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide HCl. When the cells were treated with the drug just before the beginning of cytokinesis, the drug also inhibited the localization of p85 and calmodulin to the division plane, and the formation of the contractile ring and division furrow. Therefore, we propose that the Ca(2+)/calmodulin signal and its target protein p85 cooperatively regulate an initiation of cytokinesis and may be also concerned with the progression of cytokinesis in Tetrahymena.
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  M. E. Jacobs, L. V. DeSouza, H. Samaranayake, R. E. Pearlman, K. W. M. Siu, and L. A. Klobutcher The Tetrahymena thermophila Phagosome Proteome Eukaryot. Cell, December 1, 2006; 5(12): 1990 - 2000. [Abstract] [Full Text] [PDF]
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 H. Ueno, Y. Iwataki, and O. Numata Homologues of Radial Spoke Head Proteins Interact with Ca2+/Calmodulin in Tetrahymena Cilia J. Biochem., October 1, 2006; 140(4): 525 - 533. [Abstract] [Full Text] [PDF]
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F. Bunai, K. Ando, H. Ueno, and O. Numata Tetrahymena Eukaryotic Translation Elongation Factor 1A (eEF1A) Bundles Filamentous Actin through Dimer Formation J. Biochem., September 1, 2006; 140(3): 393 - 399. [Abstract] [Full Text] [PDF]
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N. E. Williams, C.-C. Tsao, J. Bowen, G. L. Hehman, R. J. Williams, and J. Frankel The Actin Gene ACT1 Is Required for Phagocytosis, Motility, and Cell Separation of Tetrahymena thermophila Eukaryot. Cell, March 1, 2006; 5(3): 555 - 567. [Abstract] [Full Text] [PDF]
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 A. Nagasaki and T. Q.P. Uyeda DWWA, a Novel Protein Containing Two WW Domains and an IQ Motif, Is Required for Scission of the Residual Cytoplasmic Bridge during Cytokinesis in Dictyostelium Mol. Biol. Cell, February 1, 2004; 15(2): 435 - 446. [Abstract] [Full Text] [PDF]
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S. Shirayama and O. Numata Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner J. Biochem., October 1, 2003; 134(4): 591 - 598. [Abstract] [Full Text] [PDF]
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