spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, S. M.
Right arrow Articles by Turner, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, S. M.
Right arrow Articles by Turner, C. E.

Journal of Cell Science, Vol 112, Issue 2 181-190, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin

SM Thomas, M Hagel and CE Turner
Department of Medicine, Cancer Biology Program, Beth Israel Deaconess Medical Center/Harvard Medical School, Boston, MA 02215, USA. sthomas@bidmc.harvard.edu.

Paxillin is a focal adhesion scaffolding protein which was originally identified as a substrate of the oncogenic tyrosine kinase, v-src. Paxillin has been proposed to be involved in regulation of focal adhesion dynamics. Two alternatively spliced mouse paxillin cDNAs were cloned and in the process, a paxillin-related protein, Hic-5, was also identified. Cloning and characterization of Hic-5 indicates that this protein shares extensive homology with paxillin. Although Hic-5 was originally characterized as a TGF-beta-inducible gene and proposed to be a transcription factor involved in senescence, the studies here demonstrate that Hic-5 is localized to focal adhesion in REF52 cells and can interact with the focal adhesion proteins, Fak, Frnk, and vinculin. In addition, like paxillin, Hic-5 can bind to a negative regulator of Src PTKs, csk but does not bind to the adaptor protein Crk. Like paxillin, localization of this protein to focal adhesions is mediated primarily by the LIM domains; however, sequences outside the LIM domains also play a minor role in focal adhesion targeting. These results suggest that Hic-5 like paxillin could be involved in regulation of focal adhesion dynamics and raise the possibility that Hic-5 and paxillin could have overlapping or opposing functions in the overall regulation of cell growth and differentiation.


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
S. Kaulfuss, M. Grzmil, B. Hemmerlein, P. Thelen, S. Schweyer, J. Neesen, L. Bubendorf, A. G. Glass, H. Jarry, B. Auber, et al.
Leupaxin, a Novel Coactivator of the Androgen Receptor, Is Expressed in Prostate Cancer and Plays a Role in Adhesion and Invasion of Prostate Carcinoma Cells
Mol. Endocrinol., July 1, 2008; 22(7): 1606 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Wade, N. Brimer, and S. Vande Pol
Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin
J. Virol., June 15, 2008; 82(12): 5962 - 5966.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Srinivasan, S. Forman, R. A. Quinlan, J. Ohanian, and V. Ohanian
Regulation of contractility by Hsp27 and Hic-5 in rat mesenteric small arteries
Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H961 - H969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. H. Lee, U. S. Kayyali, A. M. Sousa, T. Rajan, R. J. Lechleider, and R. M. Day
Transforming Growth Factor-beta1 Effects on Endothelial Monolayer Permeability Involve Focal Adhesion Kinase/Src
Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 485 - 493.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. J. Stanton, B. P. McSharry, C. R. Rickards, E. C. Y. Wang, P. Tomasec, and G. W. G. Wilkinson
Cytomegalovirus Destruction of Focal Adhesions Revealed in a High-Throughput Western Blot Analysis of Cellular Protein Expression
J. Virol., August 1, 2007; 81(15): 7860 - 7872.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Avraamides, M. E. Bromberg, J. P. Gaughan, S. M. Thomas, A. Y. Tsygankov, and T. S. Panetti
Hic-5 promotes endothelial cell migration to lysophosphatidic acid
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H193 - H203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Mori, M. Asakawa, M. Hayashi, M. Imura, T. Ohki, E. Hirao, J.-r. Kim-Kaneyama, K. Nose, and M. Shibanuma
Oligomerizing Potential of a Focal Adhesion LIM Protein Hic-5 Organizing a Nuclear-Cytoplasmic Shuttling Complex
J. Biol. Chem., August 4, 2006; 281(31): 22048 - 22061.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Saelzler, C. C. Spackman, Y. Liu, L. C. Martinez, J. P. Harris, and M. K. Abe
ERK8 Down-regulates Transactivation of the Glucocorticoid Receptor through Hic-5
J. Biol. Chem., June 16, 2006; 281(24): 16821 - 16832.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. A. Tumbarello, M. C. Brown, S. E. Hetey, and C. E. Turner
Regulation of paxillin family members during epithelial-mesenchymal transformation: a putative role for paxillin {delta}
J. Cell Sci., October 15, 2005; 118(20): 4849 - 4863.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. G. Arias-Salgado, F. Haj, C. Dubois, B. Moran, A. Kasirer-Friede, B. C. Furie, B. Furie, B. G. Neel, and S. J. Shattil
PTP-1B is an essential positive regulator of platelet integrin signaling
J. Cell Biol., August 29, 2005; 170(5): 837 - 845.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H.-N. Fournier, S. Dupe-Manet, D. Bouvard, F. Luton, S. Degani, M. R. Block, S. F. Retta, and C. Albiges-Rizo
Nuclear Translocation of Integrin Cytoplasmic Domain-associated Protein 1 Stimulates Cellular Proliferation
Mol. Biol. Cell, April 1, 2005; 16(4): 1859 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J.-r. Kim-Kaneyama, W. Suzuki, K. Ichikawa, T. Ohki, Y. Kohno, M. Sata, K. Nose, and M. Shibanuma
Uni-axial stretching regulates intracellular localization of Hic-5 expressed in smooth-muscle cells in vivo
J. Cell Sci., March 1, 2005; 118(5): 937 - 949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Wang, K. Song, T. L. Sponseller, and D. Danielpour
Novel Function of Androgen Receptor-associated Protein 55/Hic-5 as a Negative Regulator of Smad3 Signaling
J. Biol. Chem., February 18, 2005; 280(7): 5154 - 5162.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. V. Sharp, F. Munoz, D. Bourboulia, N. Presneau, E. Darai, H.-W. Wang, M. Cannon, D. N. Butcher, A. G. Nicholson, G. Klein, et al.
LIM domains-containing protein 1 (LIMD1), a tumor suppressor encoded at chromosome 3p21.3, binds pRB and represses E2F-driven transcription
PNAS, November 23, 2004; 101(47): 16531 - 16536.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. C. Brown and C. E. Turner
Paxillin: Adapting to Change
Physiol Rev, October 1, 2004; 84(4): 1315 - 1339.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X.-D. Gao, J. P. Caviston, S. E. Tcheperegine, and E. Bi
Pxl1p, a Paxillin-like Protein in Saccharomyces cerevisiae, May Coordinate Cdc42p and Rho1p Functions during Polarized Growth
Mol. Biol. Cell, September 1, 2004; 15(9): 3977 - 3985.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Rahman, H. Miyamoto, and C. Chang
Androgen Receptor Coregulators in Prostate Cancer: Mechanisms and Clinical Implications
Clin. Cancer Res., April 1, 2004; 10(7): 2208 - 2219.
[Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Kasai, J. Guerrero-Santoro, R. Friedman, E. S. Leman, R. H. Getzenberg, and D. B. DeFranco
The Group 3 LIM Domain Protein Paxillin Potentiates Androgen Receptor Transactivation in Prostate Cancer Cell Lines
Cancer Res., August 15, 2003; 63(16): 4927 - 4935.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Didier, L. Broday, A. Bhoumik, S. Israeli, S. Takahashi, K. Nakayama, S. M. Thomas, C. E. Turner, S. Henderson, H. Sabe, et al.
RNF5, a RING Finger Protein That Regulates Cell Motility by Targeting Paxillin Ubiquitination and Altered Localization
Mol. Cell. Biol., August 1, 2003; 23(15): 5331 - 5345.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
Y. Zhuang, T. N. Faria, P. Chambon, and L. J. Gudas
Identification and Characterization of Retinoic Acid Receptor {beta}2 Target Genes in F9 Teratocarcinoma Cells
Mol. Cancer Res., June 1, 2003; 1(8): 619 - 630.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. B. Mercer, D. B. Flaherty, R. K. Miller, H. Qadota, T. L. Tinley, D. G. Moerman, and G. M. Benian
Caenorhabditis elegans UNC-98, a C2H2 Zn Finger Protein, Is a Novel Partner of UNC-97/PINCH in Muscle Adhesion Complexes
Mol. Biol. Cell, June 1, 2003; 14(6): 2492 - 2507.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Yuminamochi, Y. Yatomi, M. Osada, T. Ohmori, Y. Ishii, K. Nakazawa, S. Hosogaya, and Y. Ozaki
Expression of the LIM Proteins Paxillin and Hic-5 in Human Tissues
J. Histochem. Cytochem., April 1, 2003; 51(4): 513 - 522.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Shibanuma, J.-r. Kim-Kaneyama, K. Ishino, N. Sakamoto, T. Hishiki, K. Yamaguchi, K. Mori, J.-i. Mashimo, and K. Nose
Hic-5 Communicates between Focal Adhesions and the Nucleus through Oxidant-Sensitive Nuclear Export Signal
Mol. Biol. Cell, March 1, 2003; 14(3): 1158 - 1171.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. R. Petit, S. M. P. Meulemans, and W. J. M. Van de Ven
The Focal Adhesion and Nuclear Targeting Capacity of the LIM-containing Lipoma-preferred Partner (LPP) Protein
J. Biol. Chem., January 17, 2003; 278(4): 2157 - 2168.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, K. Chen, L. Guo, and C. Wu
Characterization of PINCH-2, a New Focal Adhesion Protein That Regulates the PINCH-1-ILK Interaction, Cell Spreading, and Migration
J. Biol. Chem., October 4, 2002; 277(41): 38328 - 38338.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. M. Carneiro, S. L. Ingram, J.-M. Beaulieu, A. Sweeney, S. G. Amara, S. M. Thomas, M. G. Caron, and G. E. Torres
The Multiple LIM Domain-Containing Adaptor Protein Hic-5 Synaptically Colocalizes and Interacts with the Dopamine Transporter
J. Neurosci., August 15, 2002; 22(16): 7045 - 7054.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. A. Heinlein and C. Chang
Androgen Receptor (AR) Coregulators: An Overview
Endocr. Rev., April 1, 2002; 23(2): 175 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Hagel, E. L. George, A. Kim, R. Tamimi, S. L. Opitz, C. E. Turner, A. Imamoto, and S. M. Thomas
The Adaptor Protein Paxillin Is Essential for Normal Development in the Mouse and Is a Critical Transducer of Fibronectin Signaling
Mol. Cell. Biol., February 1, 2002; 22(3): 901 - 915.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Jia, R. F. Ransom, M. Shibanuma, C. Liu, M. J. Welsh, and W. E. Smoyer
Identification and Characterization of hic-5/ARA55 as an hsp27 Binding Protein
J. Biol. Chem., October 19, 2001; 276(43): 39911 - 39918.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Nishiya, K. Tachibana, M. Shibanuma, J.-i. Mashimo, and K. Nose
Hic-5-Reduced Cell Spreading on Fibronectin: Competitive Effects between Paxillin and Hic-5 through Interaction with Focal Adhesion Kinase
Mol. Cell. Biol., August 15, 2001; 21(16): 5332 - 5345.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Mehta, D. D. Tang, M.-F. Wu, S. Atkinson, and S. J. Gunst
Role of Rho in Ca2+-insensitive contraction and paxillin tyrosine phosphorylation in smooth muscle
Am J Physiol Cell Physiol, August 1, 2000; 279(2): C308 - C318.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Yang, J. Guerrero, H. Hong, D. B. DeFranco, and M. R. Stallcup
Interaction of the tau 2 Transcriptional Activation Domain of Glucocorticoid Receptor with a Novel Steroid Receptor Coactivator, Hic-5, Which Localizes to Both Focal Adhesions and the Nuclear Matrix
Mol. Biol. Cell, June 1, 2000; 11(6): 2007 - 2018.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
Y. Shen, P. Lyons, M. Cooley, D. Davidson, A. Veillette, R. Salgia, J. D. Griffin, and M. D. Schaller
The Noncatalytic Domain of Protein-tyrosine Phosphatase-PEST Targets Paxillin for Dephosphorylation in Vivo
J. Biol. Chem., January 14, 2000; 275(2): 1405 - 1413.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Thomas, M. A. Cooley, J. M. Broome, R. Salgia, J. D. Griffin, C. R. Lombardo, and M. D. Schaller
The Role of Focal Adhesion Kinase Binding in the Regulation of Tyrosine Phosphorylation of Paxillin
J. Biol. Chem., December 17, 1999; 274(51): 36684 - 36692.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. E. Turner, M. C. Brown, J. A. Perrotta, M.C. Riedy, S. N. Nikolopoulos, A. R. McDonald, S. Bagrodia, S. Thomas, and P. S. Leventhal
Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF-GAP Protein: A Role in Cytoskeletal Remodeling
J. Cell Biol., May 17, 1999; 145(4): 851 - 863.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Wixler, D. Geerts, E. Laplantine, D. Westhoff, N. Smyth, M. Aumailley, A. Sonnenberg, and M. Paulsson
The LIM-only Protein DRAL/FHL2 Binds to the Cytoplasmic Domain of Several alpha and beta Integrin Chains and Is Recruited to Adhesion Complexes
J. Biol. Chem., October 20, 2000; 275(43): 33669 - 33678.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Nix, J. Fradelizi, S. Bockholt, B. Menichi, D. Louvard, E. Friederich, and M. C. Beckerle
Targeting of Zyxin to Sites of Actin Membrane Interaction and to the Nucleus
J. Biol. Chem., September 7, 2001; 276(37): 34759 - 34767.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. E. Aplin and R.L. Juliano
Regulation of nucleocytoplasmic trafficking by cell adhesion receptors and the cytoskeleton
J. Cell Biol., October 15, 2001; 155(2): 187 - 192.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999