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Journal of Cell Science, Vol 112, Issue 16 2705-2714, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Quantitative measurement of mammalian chromosome mitotic loss rates using the green fluorescent protein

EM Burns, L Christopoulou, P Corish and C Tyler-Smith
CRC Chromosome Molecular Biology Group, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

We have measured the mitotic loss rates of mammalian chromosomes in cultured cells. The green fluorescent protein (GFP) gene was incorporated into a non-essential chromosome so that cells containing the chromosome fluoresced green, while those lacking it did not. The proportions of fluorescent and non-fluorescent cells were measured by fluorescence activated cell sorter (FACS) analysis. Loss rates ranged from 0.005% to 0.20% per cell division in mouse LA-9 cells, and from 0.02% to 0.40% in human HeLa cells. The rate of loss was elevated by treatment with aneugens, demonstrating that the system rapidly identifies agents which induce chromosome loss in mammalian cells.


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© The Company of Biologists Ltd 1999