|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 13 2155-2165, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
S Bassnett, H Missey and I Vucemilo
Department of Ophthalmology, Washington University School of Medicine, Box 8096, St Louis, MO 63110-1031, USA. bassnetts@am.seer.wustl.edu
Lens fiber cells are transparent, highly elongated, epithelial cells. Because of their unusual length these cells represent a novel model system to investigate aspects of epithelial cell polarity. In this study, we examined the fiber cell basal membrane complex (BMC). The BMC anchors fiber cells to the lens capsule and facilitates their migration across the capsule. Confocal microscopy revealed that bundled actin filaments converge beneath the center of each BMC and insert into the lateral membrane at points enriched in N-cadherin. Two other contractile proteins, caldesmon and myosin, were enriched in the BMC, co-localizing with f-actin bundles. The actin/N-cadherin complex formed a hexagonal lattice, cradling the posterior face of the lens. Removal of the capsule caused the tips of the fiber cells to break off, remaining attached to the stripped capsule. This provided a method for assaying cell adhesion and purifying BMC components. Fiber cell adhesion required Mg2+ and/or Ca2+ and was disrupted by incubation with beta1 integrin antibody. BMC proteins were compared with samples from the neighboring lateral membrane. Although some components were common to both samples, others were unique to the BMC. Furthermore, some lateral membrane proteins, most notably lens major intrinsic protein (MIP), were excluded from the BMC. Western blotting of BMC preparations identified several structural proteins originally found in focal adhesions and two kinases, FAK and MLCK, previously undescribed in the lens. These data suggest that the BMC constitutes a distinct membrane domain in the lens. The structural organization of the BMC suggests a role in shaping the posterior lens face and hence the refractive properties of the eye.
This article has been cited by other articles:
|
|
 |
|
  R. Bornheim, M. Muller, U. Reuter, H. Herrmann, H. Bussow, and T. M. Magin A dominant vimentin mutant upregulates Hsp70 and the activity of the ubiquitin-proteasome system, and causes posterior cataracts in transgenic mice J. Cell Sci., November 15, 2008; 121(22): 3737 - 3746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Landgren, A. Blixt, and P. Carlsson Persistent FoxE3 Expression Blocks Cytoskeletal Remodeling and Organelle Degradation during Lens Fiber Differentiation Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4269 - 4277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Jensen, R. O. Dror, H. Xu, D. W. Borhani, I. T. Arkin, M. P. Eastwood, and D. E. Shaw Dynamic control of slow water transport by aquaporin 0: Implications for hydration and junction stability in the eye lens PNAS, September 23, 2008; 105(38): 14430 - 14435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lee and J. M. Gross Laminin {beta}1 and {gamma}1 Containing Laminins Are Essential for Basement Membrane Integrity in the Zebrafish Eye Invest. Ophthalmol. Vis. Sci., June 1, 2007; 48(6): 2483 - 2490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-D. Hsu, S. Kymes, and J. M. Petrash A Transgenic Mouse Model for Human Autosomal Dominant Cataract Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 2036 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Ledee, C. Y. Gao, R. Seth, R. N. Fariss, B. K. Tripathi, and P. S. Zelenka A Specific Interaction between Muskelin and the Cyclin-dependent Kinase 5 Activator p39 Promotes Peripheral Localization of Muskelin J. Biol. Chem., June 3, 2005; 280(22): 21376 - 21383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Elamaa, R. Sormunen, M. Rehn, R. Soininen, and T. Pihlajaniemi Endostatin Overexpression Specifically in the Lens and Skin Leads to Cataract and Ultrastructural Alterations in Basement Membranes Am. J. Pathol., January 1, 2005; 166(1): 221 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Grove, G. Demyanenko, A. Echarri, P. A. Zipfel, M. E. Quiroz, R. M. Rodriguiz, M. Playford, S. A. Martensen, M. R. Robinson, W. C. Wetsel, et al. Abi2-Deficient Mice Exhibit Defective Cell Migration, Aberrant Dendritic Spine Morphogenesis, and Deficits in Learning and Memory Mol. Cell. Biol., December 15, 2004; 24(24): 10905 - 10922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Foley, H. Rosenbaum, and A. E. Griep Temporal Regulation of VEID-7-amino-4-trifluoromethylcoumarin Cleavage Activity and Caspase-6 Correlates with Organelle Loss during Lens Development J. Biol. Chem., July 30, 2004; 279(31): 32142 - 32150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Straub, J. Boda, C. Kuhn, M. Schnoelzer, U. Korf, T. Kempf, H. Spring, M. Hatzfeld, and W. W. Franke A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells J. Cell Sci., December 15, 2003; 116(24): 4985 - 4995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. I. Shestopalov and S. Bassnett Development of a macromolecular diffusion pathway in the lens J. Cell Sci., October 15, 2003; 116(20): 4191 - 4199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Masai, Z. Lele, M. Yamaguchi, A. Komori, A. Nakata, Y. Nishiwaki, H. Wada, H. Tanaka, Y. Nojima, M. Hammerschmidt, et al. N-cadherin mediates retinal lamination, maintenance of forebrain compartments and patterning of retinal neurites Development, June 1, 2003; 130(11): 2479 - 2494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Negash, H.-S. Wang, C. Gao, D. Ledee, and P. Zelenka Cdk5 regulates cell-matrix and cell-cell adhesion in lens epithelial cells J. Cell Sci., May 15, 2002; 115(10): 2109 - 2117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Choh, J. G. Sivak, and S. D. Meriney A Physiological Model to Measure Effects of Age on Lenticular Accommodation and Spherical Aberration in Chickens Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 92 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. More, F.-P. Kirsch, and F. G. Rathjen Targeted ablation of NrCAM or ankyrin-B results in disorganized lens fibers leading to cataract formation J. Cell Biol., July 9, 2001; 154(1): 187 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Beebe, O. Vasiliev, J. Guo, Y.-B. Shui, and S. Bassnett Changes in Adhesion Complexes Define Stages in the Differentiation of Lens Fiber Cells Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 727 - 734. [Abstract] [Full Text]
|
|
|
|
|
|
V. I. Shestopalov and S. Bassnett Three-Dimensional Organization of Primary Lens Fiber Cells Invest. Ophthalmol. Vis. Sci., March 1, 2000; 41(3): 859 - 863. [Abstract] [Full Text]
|
|
|
|
|
|
M. Duncan, Z Kozmik, K Cveklova, J Piatigorsky, and A Cvekl Overexpression of PAX6(5a) in lens fiber cells results in cataract and upregulation of (alpha)5(beta)1 integrin expression J. Cell Sci., January 9, 2000; 113(18): 3173 - 3185. [Abstract] [PDF]
|
|
|
|
 |
|
 A. SHIELS, S. BASSNETT, K. VARADARAJ, R. MATHIAS, K. AL-GHOUL, J. KUSZAK, D. DONOVIEL, S. LILLEBERG, G. FRIEDRICH, and B. ZAMBROWICZ Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice Physiol Genomics, December 21, 2001; 7(2): 179 - 186. [Abstract] [Full Text] [PDF]
|
|
