|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 13 2125-2136, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
JD Wulfkuhle, IE Donina, NH Stark, RK Pope, KN Pestonjamasp, ML Niswonger and EJ Luna
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
A growing number of actin-associated membrane proteins have been implicated in motile processes, adhesive interactions, and signal transduction to the cell nucleus. We report here that supervillin, an F-actin binding protein originally isolated from bovine neutrophil plasma membranes, contains functional nuclear targeting signals and localizes at or near vinculin-containing focal adhesion plaques in COS7-2 and CV1 cells. Overexpression of full-length supervillin in these cells disrupts the integrity of focal adhesion plaques and results in increased levels of F-actin and vinculin. Localization studies of chimeric proteins containing supervillin sequences fused with the enhanced green fluorescent protein indicate that: (1) the amino terminus promotes F-actin binding, targeting to focal adhesions, and limited nuclear localization; (2) the dominant nuclear targeting signal is in the center of the protein; and (3) the carboxy-terminal villin/gelsolin homology domain of supervillin does not, by itself, bind tightly to the actin cytoskeleton in vivo. Overexpression of chimeras containing both the amino-terminal F-actin binding site(s) and the dominant nuclear targeting signal results in the formation of large nuclear bundles containing F-actin, supervillin, and lamin. These results suggest that supervillin may contribute to cytoarchitecture in the nucleus, as well as at the plasma membrane.
This article has been cited by other articles:
|
|
 |
|
  N. Takizawa, R. Ikebe, M. Ikebe, and E. J. Luna Supervillin slows cell spreading by facilitating myosin II activation at the cell periphery J. Cell Sci., November 1, 2007; 120(21): 3792 - 3803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Barrientos, D. Frank, K. Kuwahara, S. Bezprozvannaya, G. C. T. Pipes, R. Bassel-Duby, J. A. Richardson, H. A. Katus, E. N. Olson, and N. Frey Two Novel Members of the ABLIM Protein Family, ABLIM-2 and -3, Associate with STARS and Directly Bind F-actin J. Biol. Chem., March 16, 2007; 282(11): 8393 - 8403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Takizawa, T. C. Smith, T. Nebl, J. L. Crowley, S. J. Palmieri, L. M. Lifshitz, A. G. Ehrhardt, L. M. Hoffman, M. C. Beckerle, and E. J. Luna Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 J. Cell Biol., July 31, 2006; 174(3): 447 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Loomis, A. E. Kelly, L. Zheng, B. Changyaleket, G. Sekerkova, E. Mugnaini, A. Ferreira, R. D. Mullins, and J. R. Bartles Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells J. Cell Sci., April 15, 2006; 119(8): 1655 - 1665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takahashi, L. Li, M. Kamiryo, T. Asteriou, A. Moustakas, H. Yamashita, and P. Heldin Hyaluronan Fragments Induce Endothelial Cell Differentiation in a CD44- and CXCL1/GRO1-dependent Manner J. Biol. Chem., June 24, 2005; 280(25): 24195 - 24204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. De Corte, K. Van Impe, E. Bruyneel, C. Boucherie, M. Mareel, J. Vandekerckhove, and J. Gettemans Increased importin-{beta}-dependent nuclear import of the actin modulating protein CapG promotes cell invasion J. Cell Sci., October 15, 2004; 117(22): 5283 - 5292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Gangopadhyay, N. Takizawa, C. Gallant, A. L. Barber, H.-D. Je, T. C. Smith, E. J. Luna, and K. G. Morgan Smooth muscle archvillin: a novel regulator of signaling and contractility in vascular smooth muscle J. Cell Sci., October 1, 2004; 117(21): 5043 - 5057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, N. Takizawa, J. L. Crowley, S. W. Oh, C. L. Gatto, T. Kambara, O. Sato, X.-d. Li, M. Ikebe, and E. J. Luna F-actin and Myosin II Binding Domains in Supervillin J. Biol. Chem., November 14, 2003; 278(46): 46094 - 46106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Athman, D. Louvard, and S. Robine Villin Enhances Hepatocyte Growth Factor-induced Actin Cytoskeleton Remodeling in Epithelial Cells Mol. Biol. Cell, November 1, 2003; 14(11): 4641 - 4653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nishimura, H.-J. Ting, Y. Harada, T. Tokizane, N. Nonomura, H.-Y. Kang, H.-C. Chang, S. Yeh, H. Miyamoto, M. Shin, et al. Modulation of Androgen Receptor Transactivation by Gelsolin: A Newly Identified Androgen Receptor Coregulator Cancer Res., August 15, 2003; 63(16): 4888 - 4894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gloss, F. Rivero, N. Khaire, R. Muller, W. F. Loomis, M. Schleicher, and A. A. Noegel Villidin, a Novel WD-repeat and Villin-related Protein from Dictyostelium, Is Associated with Membranes and the Cytoskeleton Mol. Biol. Cell, July 1, 2003; 14(7): 2716 - 2727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Oh, R. K. Pope, K. P. Smith, J. L. Crowley, T. Nebl, J. B. Lawrence, and E. J. Luna Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton J. Cell Sci., June 1, 2003; 116(11): 2261 - 2275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Van Impe, V. De Corte, L. Eichinger, E. Bruyneel, M. Mareel, J. Vandekerckhove, and J. Gettemans The Nucleo-cytoplasmic Actin-binding Protein CapG Lacks a Nuclear Export Sequence Present in Structurally Related Proteins J. Biol. Chem., May 9, 2003; 278(20): 17945 - 17952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nebl, K. N. Pestonjamasp, J. D. Leszyk, J. L. Crowley, S. W. Oh, and E. J. Luna Proteomic Analysis of a Detergent-resistant Membrane Skeleton from Neutrophil Plasma Membranes J. Biol. Chem., November 1, 2002; 277(45): 43399 - 43409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Heinlein and C. Chang Androgen Receptor (AR) Coregulators: An Overview Endocr. Rev., April 1, 2002; 23(2): 175 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Weins, K. Schwarz, C. Faul, L. Barisoni, W. A. Linke, and P. Mundel Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein J. Cell Biol., October 29, 2001; 155(3): 393 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Davy, H. Campbell, S Fountain, D de Jong, and M. Crouch The flightless I protein colocalizes with actin- and microtubule-based structures in motile Swiss 3T3 fibroblasts: evidence for the involvement of PI 3-kinase and Ras-related small GTPases J. Cell Sci., January 2, 2001; 114(3): 549 - 562. [Abstract] [PDF]
|
|
|
|
|
|
M. M. Parast and C. A. Otey Characterization of Palladin, a Novel Protein Localized to Stress Fibers and Cell Adhesions J. Cell Biol., August 7, 2000; 150(3): 643 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Drachman, G. P. Jarvik, and M. G. Mehaffey Autosomal dominant thrombocytopenia: incomplete megakaryocyte differentiation and linkage to human chromosome 10 Blood, July 1, 2000; 96(1): 118 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Ting, S. Yeh, K. Nishimura, and C. Chang Supervillin associates with androgen receptor and modulates its transcriptional activity PNAS, January 22, 2002; 99(2): 661 - 666. [Abstract] [Full Text] [PDF]
|
|
