spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Csikos, G.
Right arrow Articles by Sass, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Csikos, G.
Right arrow Articles by Sass, M.

Journal of Cell Science, Vol 112, Issue 13 2113-2124, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Insect cuticle, an in vivo model of protein trafficking

G Csikos, K Molnar, NH Borhegyi, GC Talian and M Sass
Department of General Zoology, Eotvos Lorand University, Budapest, Hungary.

In the course of this study more than 20 proteins have been isolated from the larval cuticle of Manduca sexta. Synthesis, secretion, transport and accumulation of four particular proteins, representative members of four characteristic groups, were followed during metamorphosis by immunoblot and immuncytochemical methods and are described in detail in this paper. We established that only some of the proteins of the soft cuticle of Lepidopteran larvae are synthesized in epidermal cells at the beginning of the larval stages and are digested during the moulting period (MsCP29). Other proteins (MsCP30/11) are secreted into the cuticle by the epidermal cells in different forms during various developmental stages. Some proteins are secreted apically during the feeding period, but before ecdysis they are then taken up by epidermal cells and transported in a basolateral direction back into the hemolymph and saved in an immunologically intact form by the fat body cells (MsCP12.3). Some cuticle proteins have a non-epidermal origin. They are transported from the hemolymph into the cuticle. Before and during ecdysis these molecules reappear in the hemolymph and are detectable again in the pupal cuticle (MsCP78). Our data prove that the cuticle is not a non-living part of the insect body: it is not only an inert, protective armor, but maintains a continuous and dynamic metabolic connection with the other organs of the organism.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
A. Dombrovsky, N. Gollop, S. Chen, N. Chejanovsky, and B. Raccah
In vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae
J. Gen. Virol., May 1, 2007; 88(5): 1602 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Asano and M. Ashida
Transepithelially Transported Pro-phenoloxidase in the Cuticle of the Silkworm, Bombyx mori. IDENTIFICATION OF ITS METHIONYL RESIDUES OXIDIZED TO METHIONINE SULFOXIDES
J. Biol. Chem., March 30, 2001; 276(14): 11113 - 11125.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Asano and M. Ashida
Cuticular Pro-phenoloxidase of the Silkworm, Bombyx mori. PURIFICATION AND DEMONSTRATION OF ITS TRANSPORT FROM HEMOLYMPH
J. Biol. Chem., March 30, 2001; 276(14): 11100 - 11112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Spada, H. Steen, C. Troedsson, T. Kallesoe, E. Spriet, M. Mann, and E. M. Thompson
Molecular Patterning of the Oikoplastic Epithelium of the Larvacean Tunicate Oikopleura dioica
J. Biol. Chem., June 1, 2001; 276(23): 20624 - 20632.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1999