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Journal of Cell Science, Vol 112, Issue 11 1797-1801, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
SO Kolset, TT Vuong and K Prydz
Institute for Nutrition Research, University of Oslo, Box 1046 Blindern, Norway. s.o.kolset@basalmed.uio.no
Sugar moieties have been shown to contain sufficient and necessary information to target examples of secreted and transmembrane glycoproteins to the apical surface of epithelial MDCK cells. We have investigated if the sugar chains of proteoglycans, the glycosaminoglycans, also contain structural determinants for apical transport. Here we show that although 75% of the proteoglycan secretion from MDCK cell monolayers is into the basolateral medium, 75% of the proteoglycans of the chondroitin sulphate type are secreted apically. The sorting information in the chondroitin sulphate proteoglycans is localized to the sugar chains, since protein-free chondroitin sulphate chains, initiated on hexyl beta-D-thioxyloside, were also predominantly secreted to the apical medium.
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  G. Dick, F. Grondahl, and K. Prydz Overexpression of the 3'-Phosphoadenosine 5'-Phosphosulfate (PAPS) Transporter 1 Increases Sulfation of Chondroitin Sulfate in the Apical Pathway of MDCK II Cells Glycobiology, January 1, 2008; 18(1): 53 - 65. [Abstract] [Full Text] [PDF]
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H. Tveit, G. Dick, V. Skibeli, and K. Prydz A Proteoglycan Undergoes Different Modifications en Route to the Apical and Basolateral Surfaces of Madin-Darby Canine Kidney Cells J. Biol. Chem., August 19, 2005; 280(33): 29596 - 29603. [Abstract] [Full Text] [PDF]
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K. Fjeldstad, M. E. Pedersen, T. T. Vuong, S. O. Kolset, L. M. Nordstrand, and K. Prydz Sulfation in the Golgi Lumen of Madin-Darby Canine Kidney Cells Is Inhibited by Brefeldin A and Depends on a Factor Present in the Cytoplasm and on Golgi Membranes J. Biol. Chem., September 20, 2002; 277(39): 36272 - 36279. [Abstract] [Full Text] [PDF]
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K. Mardberg, E. Trybala, F. Tufaro, and T. Bergstrom Herpes simplex virus type 1 glycoprotein C is necessary for efficient infection of chondroitin sulfate-expressing gro2C cells J. Gen. Virol., February 1, 2002; 83(2): 291 - 300. [Abstract] [Full Text] [PDF]
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 L. Shalamanova, B. Kubler, J.-G. Scharf, and T. Braulke MDCK cells secrete neutral proteases cleaving insulin-like growth factor-binding protein-2 to -6 Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1221 - E1229. [Abstract] [Full Text] [PDF]
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 K Prydz and K. Dalen Synthesis and sorting of proteoglycans J. Cell Sci., January 1, 2000; 113(2): 193 - 205. [Abstract] [PDF]
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F. Safaiyan, S. O. Kolset, K. Prydz, E. Gottfridsson, U. Lindahl, and M. Salmivirta Selective Effects of Sodium Chlorate Treatment on the Sulfation of Heparan Sulfate J. Biol. Chem., December 17, 1999; 274(51): 36267 - 36273. [Abstract] [Full Text] [PDF]
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