|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 10 1519-1528, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
SM Richards, ME Jaconi, G Vassort and M Puceat
INSERM U-390, Laboratoire de Physiopathologie Cardiovasculaire, CHU Arnaud de Villeneuve, Montpellier, France.
The anion exchangers (AE) are encoded by a multigenic family that comprises at least three genes, AE1, AE2 and AE3, and numerous splicoforms. Besides regulating intracellular pH (pHi) via the Cl-/HCO3- exchange, the AEs exert various cellular functions including generation of a senescent antigen, anchorage of the cytoskeleton to the membrane and regulation of metabolism. Most cells express several AE isoforms. Despite the key role of this family of proteins, little is known about the function of specific AE isoforms in any tissue, including the heart. We therefore chose isolated cardiac cells, in which a tight control of pHi is mandatory for the excitation-contraction coupling process, to thoroughly investigate the expression of the AE genes at both the mRNA and protein levels. RT-PCR revealed the presence of AE1, AE2 and AE3 mRNAs in both neonatal and adult rat cardiomyocytes. AE1 is expressed both as the erythroid form (Band 3 or eAE1) and a novel alternate transcript (nAE1), which was more specifically characterized using a PCR mapping strategy. Two variants of AE2 (AE2a and AE2c) were found at the mRNA level. Cardiac as well as brain AE3 mRNAs were expressed in both neonatal and adult rat cardiomyocytes. Several AE protein isoforms were found, including a truncated form of AE1 and two AE3s, but there was no evidence of AE2 protein in adult rat cardiomyocytes. In cardiomyocytes transfected with an AE3 oligodeoxynucleotide antisense, AE3 immunoreactivity was dramatically decreased but the activity of the Cl-/HCO3- exchange was unchanged. In contrast, intracellular microinjection of blocking anti-AE1 antibodies inhibited the AE activity. Altogether, our findings suggest that a specific and novel AE1 splicoform (nAE1) mediates the cardiac Cl-/HCO3- exchange. The multiple gene and protein expression within the same cell type suggest numerous functions for this protein family.
This article has been cited by other articles:
|
|
 |
|
  B. V. Alvarez, D. M. Kieller, A. L. Quon, M. Robertson, and J. R. Casey Cardiac hypertrophy in anion exchanger 1-null mutant mice with severe hemolytic anemia Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1301 - H1312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pushkin and I. Kurtz SLC4 base (HCO3-, CO32-) transporters: classification, function, structure, genetic diseases, and knockout models Am J Physiol Renal Physiol, March 1, 2006; 290(3): F580 - F599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. V Alvarez, D. M Kieller, A. L Quon, D. Markovich, and J. R Casey Slc26a6: a cardiac chloride-hydroxyl exchanger and predominant chloride-bicarbonate exchanger of the mouse heart J. Physiol., December 15, 2004; 561(3): 721 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Cingolani, G. E. Chiappe, I. L. Ennis, P. G. Morgan, B. V. Alvarez, J. R. Casey, R. A. Dulce, N. G. Perez, and M. C. Camilion de Hurtado Influence of Na+-Independent Cl--HCO3- Exchange on the Slow Force Response to Myocardial Stretch Circ. Res., November 28, 2003; 93(11): 1082 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Frazar, J. L. Weisbein, S. M. Anderson, A. P. Cline, L. J. Garrett, G. Felsenfeld, P. G. Gallagher, and D. M. Bodine Variegated Expression from the Murine Band 3 (AE1) Promoter in Transgenic Mice Is Associated with mRNA Transcript Initiation at Upstream Start Sites and Can Be Suppressed by the Addition of the Chicken {beta}-Globin 5' HS4 Insulator Element Mol. Cell. Biol., July 15, 2003; 23(14): 4753 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.K. Stewart, M.N. Chernova, B.E. Shmukler, S. Wilhelm, and S.L. Alper Regulation of AE2-mediated Cl- Transport by Intracellular or by Extracellular pH Requires Highly Conserved Amino Acid Residues of the AE2 NH2-terminal Cytoplasmic Domain J. Gen. Physiol., October 29, 2002; 120(5): 707 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sterling, B. V. Alvarez, and J. R. Casey The Extracellular Component of a Transport Metabolon. EXTRACELLULAR LOOP 4 OF THE HUMAN AE1 Cl-/HCO3- EXCHANGER BINDS CARBONIC ANHYDRASE IV J. Biol. Chem., July 5, 2002; 277(28): 25239 - 25246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Chiappe de Cingolani, P. Morgan, C. Mundina-Weilenmann, J. Casey, J. Fujinaga, M. Camilion de Hurtado, and H. Cingolani Hyperactivity and altered mRNA isoform expression of the Cl-/HCO3- anion-exchanger in the hypertrophied myocardium Cardiovasc Res, July 1, 2001; 51(1): 71 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Papageorgiou, B. E. Shmukler, A. K. Stuart-Tilley, L. Jiang, and S. L. Alper AE anion exchangers in atrial tumor cells Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H937 - H945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Lorente, A. Lacampagne, Y. Pouzeratte, S. Richards, B. Malitschek, R. Kuhn, B. Bettler, and G. Vassort gamma -Aminobutyric acid type B receptors are expressed and functional in mammalian cardiomyocytes PNAS, July 18, 2000; 97(15): 8664 - 8669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Aimond, J.-M. Rauzier, C. Bony, and G. Vassort Simultaneous Activation of p38 MAPK and p42/44 MAPK by ATP Stimulates the K+ Current ITREK in Cardiomyocytes J. Biol. Chem., December 8, 2000; 275(50): 39110 - 39116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. V. Alvarez, J. Fujinaga, and J. R. Casey Molecular Basis for Angiotensin II-Induced Increase of Chloride/Bicarbonate Exchange in the Myocardium Circ. Res., December 7, 2001; 89(12): 1246 - 1253. [Abstract] [Full Text] [PDF]
|
|
