|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 112, Issue 10 1437-1447, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
KT Vaughan, SH Tynan, NE Faulkner, CJ Echeverri and RB Vallee
University of Massachusetts Medical School, Worcester, MA 01605, USA.
Cytoplasmic dynein is a minus end-directed microtubule motor responsible for centripetal organelle movement and several aspects of chromosome segregation. Our search for cytoplasmic dynein-interacting proteins has implicated the dynactin complex as the cytoplasmic dynein 'receptor' on organelles and kinetochores. Immunofluorescence microscopy using a total of six antibodies generated against the p150Glued, Arp1 and dynamitin subunits of dynactin revealed a novel fraction of dynactin-positive structures aligned in linear arrays along the distal segments of interphase microtubules. Dynactin staining revealed that these structures colocalized extensively with CLIP-170. Cytoplasmic dynein staining was undetectable, but extensive colocalization with dynactin became evident upon transfer to a lower temperature. Overexpression of the dynamitin subunit of dynactin removed Arp1 from microtubules but did not affect microtubule-associated p150Glued or CLIP-170 staining. Brief acetate treatment, which has been shown to affect lysosomal and endosomal traffic, also dispersed the Golgi apparatus and eliminated the microtubule-associated staining pattern. The effect on dynactin was rapidly reversible and, following acetate washout, punctate dynactin was detected at microtubule ends within 3 minutes. Together, these findings identify a region along the distal segments of microtubules where dynactin and CLIP-170 colocalize. Because CLIP-170 has been reported to mark growing microtubule ends, our results indicate a similar relationship for dynactin. The functional interaction between dynactin and cytoplasmic dynein further suggests that this these regions represent accumulations of cytoplasmic dynein cargo-loading sites involved in the early stages of minus end-directed organelle transport.
This article has been cited by other articles:
|
|
 |
|
  P. W. Grabham, G. E. Seale, M. Bennecib, D. J. Goldberg, and R. B. Vallee Cytoplasmic Dynein and LIS1 Are Required for Microtubule Advance during Growth Cone Remodeling and Fast Axonal Outgrowth J. Neurosci., May 23, 2007; 27(21): 5823 - 5834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D.-A. Tran, T. P. Marmo, A. A. Salam, S. Che, E. Finkelstein, R. Kabarriti, H. S. Xenias, R. Mazitschek, C. Hubbert, Y. Kawaguchi, et al. HDAC6 deacetylation of tubulin modulates dynamics of cellular adhesions J. Cell Sci., April 15, 2007; 120(8): 1469 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Dompierre, J. D. Godin, B. C. Charrin, F. P. Cordelieres, S. J. King, S. Humbert, and F. Saudou Histone Deacetylase 6 Inhibition Compensates for the Transport Deficit in Huntington's Disease by Increasing Tubulin Acetylation J. Neurosci., March 28, 2007; 27(13): 3571 - 3583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Kim, M. Takahashi, K. Matsuo, and Y. Ono Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex Genes Cells, March 1, 2007; 12(3): 421 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kim, S.-C. Ling, G. C. Rogers, C. Kural, P. R. Selvin, S. L. Rogers, and V. I. Gelfand Microtubule binding by dynactin is required for microtubule organization but not cargo transport J. Cell Biol., February 26, 2007; 176(5): 641 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Peris, M. Thery, J. Faure, Y. Saoudi, L. Lafanechere, J. K. Chilton, P. Gordon-Weeks, N. Galjart, M. Bornens, L. Wordeman, et al. Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends J. Cell Biol., September 11, 2006; 174(6): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. P. Efimov, J. Zhang, and X. Xiang CLIP-170 Homologue and NUDE Play Overlapping Roles in NUDF Localization in Aspergillus nidulans Mol. Biol. Cell, April 1, 2006; 17(4): 2021 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xiang A +TIP for a smooth trip J. Cell Biol., February 27, 2006; 172(5): 651 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Levy, C. J. Sumner, J. P. Caviston, M. K. Tokito, S. Ranganathan, L. A. Ligon, K. E. Wallace, B. H. LaMonte, G. G. Harmison, I. Puls, et al. A motor neuron disease-associated mutation in p150Glued perturbs dynactin function and induces protein aggregation J. Cell Biol., February 27, 2006; 172(5): 733 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Folker, B. M. Baker, and H. V. Goodson Interactions between CLIP-170, Tubulin, and Microtubules: Implications for the Mechanism of CLIP-170 Plus-End Tracking Behavior Mol. Biol. Cell, November 1, 2005; 16(11): 5373 - 5384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, C. E. Oakley, G. Chen, X. Han, B. R. Oakley, and X. Xiang Cytoplasmic Dynein's Mitotic Spindle Pole Localization Requires a Functional Anaphase-promoting Complex, {gamma}-Tubulin, and NUDF/LIS1 in Aspergillus nidulans Mol. Biol. Cell, August 1, 2005; 16(8): 3591 - 3605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Noritake, T. Watanabe, K. Sato, S. Wang, and K. Kaibuchi IQGAP1: a key regulator of adhesion and migration J. Cell Sci., May 15, 2005; 118(10): 2085 - 2092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Niccoli, A. Yamashita, P. Nurse, and M. Yamamoto The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast J. Cell Sci., November 1, 2004; 117(23): 5543 - 5556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lansbergen, Y. Komarova, M. Modesti, C. Wyman, C. C. Hoogenraad, H. V. Goodson, R. P. Lemaitre, D. N. Drechsel, E. van Munster, T. W.J. Gadella Jr., et al. Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization J. Cell Biol., October 4, 2004; (2004) jcb.200402082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Williams, C. J. Locke, A. L. Braden, K. A. Caldwell, and G. A. Caldwell Epileptic-like convulsions associated with LIS-1 in the cytoskeletal control of neurotransmitter signaling in Caenorhabditis elegans Hum. Mol. Genet., September 15, 2004; 13(18): 2043 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Badin-Larcon, C. Boscheron, J. M. Soleilhac, M. Piel, C. Mann, E. Denarier, A. Fourest-Lieuvin, L. Lafanechere, M. Bornens, and D. Job From the Cover: Suppression of nuclear oscillations in Saccharomyces cerevisiae expressing Glu tubulin PNAS, April 13, 2004; 101(15): 5577 - 5582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Noritake, M. Fukata, K. Sato, M. Nakagawa, T. Watanabe, N. Izumi, S. Wang, Y. Fukata, and K. Kaibuchi Positive Role of IQGAP1, an Effector of Rac1, in Actin-Meshwork Formation at Sites of Cell-Cell Contact Mol. Biol. Cell, March 1, 2004; 15(3): 1065 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Dujardin, L. E. Barnhart, S. A. Stehman, E. R. Gomes, G. G. Gundersen, and R. B. Vallee A role for cytoplasmic dynein and LIS1 in directed cell movement J. Cell Biol., December 22, 2003; 163(6): 1205 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Bu and L.-K. Su Characterization of Functional Domains of Human EB1 Family Proteins J. Biol. Chem., December 12, 2003; 278(50): 49721 - 49731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. King, C. L. Brown, K. C. Maier, N. J. Quintyne, and T. A. Schroer Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo Mol. Biol. Cell, December 1, 2003; 14(12): 5089 - 5097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yamamoto and Y. Hiraoka Cytoplasmic dynein in fungi: insights from nuclear migration J. Cell Sci., November 15, 2003; 116(22): 4501 - 4512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hayashi and M. Ikura Crystal Structure of the Amino-terminal Microtubule-binding Domain of End-binding Protein 1 (EB1) J. Biol. Chem., September 19, 2003; 278(38): 36430 - 36434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Grieshaber, N. A. Grieshaber, and T. Hackstadt Chlamydia trachomatis uses host cell dynein to traffic to the microtubule-organizing center in a p50 dynamitin-independent process J. Cell Sci., September 15, 2003; 116(18): 3793 - 3802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, G. Jerdeva, F. A. Yarber, S. R. da Costa, J. Xie, L. Qian, C. M. Rose, C. Mazurek, N. Kasahara, A. K. Mircheff, et al. Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells J. Cell Sci., May 15, 2003; 116(10): 2051 - 2065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Stepanova, J. Slemmer, C. C. Hoogenraad, G. Lansbergen, B. Dortland, C. I. De Zeeuw, F. Grosveld, G. van Cappellen, A. Akhmanova, and N. Galjart Visualization of Microtubule Growth in Cultured Neurons via the Use of EB3-GFP (End-Binding Protein 3-Green Fluorescent Protein) J. Neurosci., April 1, 2003; 23(7): 2655 - 2664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Ligon, S. S. Shelly, M. Tokito, and E. L.F. Holzbaur The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization Mol. Biol. Cell, April 1, 2003; 14(4): 1405 - 1417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, S. Li, R. Fischer, and X. Xiang Accumulation of Cytoplasmic Dynein and Dynactin at Microtubule Plus Ends in Aspergillus nidulans Is Kinesin Dependent Mol. Biol. Cell, April 1, 2003; 14(4): 1479 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Deacon, A. S. Serpinskaya, P. S. Vaughan, M. L. Fanarraga, I. Vernos, K. T. Vaughan, and V. I. Gelfand Dynactin is required for bidirectional organelle transport J. Cell Biol., February 3, 2003; 160(3): 297 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, J. Finley, Z.-J. Liu, S.-H. Qiu, H. Chen, C.-H. Luan, M. Carson, J. Tsao, D. Johnson, G. Lin, et al. Crystal Structure of the Cytoskeleton-associated Protein Glycine-rich (CAP-Gly) Domain J. Biol. Chem., December 6, 2002; 277(50): 48596 - 48601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Sahin, F. Neumann, O. Tureci, R. Schmits, F. Perez, and M. Pfreundschuh Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells Blood, December 1, 2002; 100(12): 4139 - 4145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Komarova, A. S. Akhmanova, S.-i. Kojima, N. Galjart, and G. G. Borisy Cytoplasmic linker proteins promote microtubule rescue in vivo J. Cell Biol., November 25, 2002; 159(4): 589 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Ji, M. Haghnia, C. Trusty, L. S. B. Goldstein, and G. Schubiger A Genetic Screen for Suppressors and Enhancers of the Drosophila Cdk1-Cyclin B Identifies Maternal Factors That Regulate Microtubule and Microfilament Stability Genetics, November 1, 2002; 162(3): 1179 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Askham, K. T. Vaughan, H. V. Goodson, and E. E. Morrison Evidence That an Interaction between EB1 and p150Glued Is Required for the Formation and Maintenance of a Radial Microtubule Array Anchored at the Centrosome Mol. Biol. Cell, October 1, 2002; 13(10): 3627 - 3645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Vaughan, P. Miura, M. Henderson, B. Byrne, and K. T. Vaughan A role for regulated binding of p150Glued to microtubule plus ends in organelle transport J. Cell Biol., July 22, 2002; 158(2): 305 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Coquelle, M. Caspi, F. P. Cordelieres, J. P. Dompierre, D. L. Dujardin, C. Koifman, P. Martin, C. C. Hoogenraad, A. Akhmanova, N. Galjart, et al. LIS1, CLIP-170's Key to the Dynein/Dynactin Pathway Mol. Cell. Biol., May 1, 2002; 22(9): 3089 - 3102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Perez, K. Pernet-Gallay, C. Nizak, H. V. Goodson, T. E. Kreis, and B. Goud CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family J. Cell Biol., February 18, 2002; 156(4): 631 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Komarova, I. A. Vorobjev, and G. G. Borisy Life cycle of MTs: persistent growth in the cell interior, asymmetric transition frequencies and effects of the cell boundary J. Cell Sci., January 9, 2002; 115(17): 3527 - 3539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kaverina, O. Krylyshkina, K. Beningo, K. Anderson, Y.-L. Wang, and J. V. Small Tensile stress stimulates microtubule outgrowth in living cells J. Cell Sci., January 6, 2002; 115(11): 2283 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Tai, J.-Z. Chuang, and C.-H. Sung Cytoplasmic Dynein Regulation by Subunit Heterogeneity and Its Role in Apical Transport J. Cell Biol., June 25, 2001; 153(7): 1499 - 1510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Fuchs and I. Karakesisoglou Bridging cytoskeletal intersections Genes & Dev., January 1, 2001; 15(1): 1 - 14. [Full Text]
|
|
|
|
|
|
A Habermann, T. Schroer, G Griffiths, and J. Burkhardt Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles J. Cell Sci., January 1, 2001; 114(1): 229 - 240. [Abstract] [PDF]
|
|
|
|
|
|
J. C. Sisson, C. Field, R. Ventura, A. Royou, and W. Sullivan Lava Lamp, a Novel Peripheral Golgi Protein, Is Required for Drosophila melanogaster Cellularization J. Cell Biol., November 13, 2000; 151(4): 905 - 918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Waterman-Storer, W. C. Salmon, and E.D. Salmon Feedback Interactions between Cell-Cell Adherens Junctions and Cytoskeletal Dynamics in Newt Lung Epithelial Cells Mol. Biol. Cell, July 1, 2000; 11(7): 2471 - 2483. [Abstract] [Full Text]
|
|
|
|
|
|
R. Saffery, D. V. Irvine, B. Griffiths, P. Kalitsis, L. Wordeman, and K.H. A. Choo Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins. Hum. Mol. Genet., January 22, 2000; 9(2): 175 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Infante, M. Stein, Y Zhai, G. Borisy, and G. Gundersen Detyrosinated (Glu) microtubules are stabilized by an ATP-sensitive plus-end cap J. Cell Sci., January 11, 2000; 113(22): 3907 - 3919. [Abstract] [PDF]
|
|
|
|
|
|
C. Valetti, D. M. Wetzel, M. Schrader, M. J. Hasbani, S. R. Gill, T. E. Kreis, and T. A. Schroer Role of Dynactin in Endocytic Traffic: Effects of Dynamitin Overexpression and Colocalization with CLIP-170 Mol. Biol. Cell, December 1, 1999; 10(12): 4107 - 4120. [Abstract] [Full Text]
|
|
|
|
|
|
A. Purohit, S. H. Tynan, R. Vallee, and S. J. Doxsey Direct Interaction of Pericentrin with Cytoplasmic Dynein Light Intermediate Chain Contributes to Mitotic Spindle Organization J. Cell Biol., November 1, 1999; 147(3): 481 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Eckley, S. R. Gill, K. A. Melkonian, J. B. Bingham, H. V. Goodson, J. E. Heuser, and T. A. Schroer Analysis of Dynactin Subcomplexes Reveals a Novel Actin-related Protein Associated with the Arp1 Minifilament Pointed End J. Cell Biol., October 18, 1999; 147(2): 307 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guilherme, M. Emoto, J. M. Buxton, S. Bose, R. Sabini, W. E. Theurkauf, J. Leszyk, and M. P. Czech Perinuclear Localization and Insulin Responsiveness of GLUT4 Requires Cytoskeletal Integrity in 3T3-L1 Adipocytes J. Biol. Chem., December 1, 2000; 275(49): 38151 - 38159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kuntziger, O. Gavet, A. Sobel, and M. Bornens Differential Effect of Two Stathmin/Op18 Phosphorylation Mutants on Xenopus Embryo Development J. Biol. Chem., June 15, 2001; 276(25): 22979 - 22984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Krylyshkina, I. Kaverina, W. Kranewitter, W. Steffen, M. C. Alonso, R. A. Cross, and J. V. Small Modulation of substrate adhesion dynamics via microtubule targeting requires kinesin-1 J. Cell Biol., January 21, 2002; 156(2): 349 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lin, P. de Carvalho, D. Kho, C.-Y. Tai, P. Pierre, G. R. Fink, and D. Pellman Polyploids require Bik1 for kinetochore-microtubule attachment J. Cell Biol., December 24, 2001; 155(7): 1173 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Perez, K. Pernet-Gallay, C. Nizak, H. V. Goodson, T. E. Kreis, and B. Goud CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family J. Cell Biol., February 18, 2002; 156(4): 631 - 642. [Abstract] [Full Text] [PDF]
|
|
