|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 9 1147-1153, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
WQ Zhao, H Li, K Yamashita, XK Guo, T Hoshino, S Yoshida, T Shinya and T Hayakawa
Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
We first confirmed an earlier immunohistochemical study showing that immunoreactive TIMP-1-like protein accumulated in the nuclei of human gingival fibroblasts (Gin-1 cells), reaching a maximum in the S phase of the cell cycle (Li, H., Nishio, K., Yamashita, K., Hayakawa, T. and Hoshino, T. (1995). Nagoya J. Med. Sci. 58, 133-142). Then we isolated this protein from a nuclear extract of Gin-1 cells and demonstrated it to be identical to human recombinant TIMP-1 by western blotting, by a sandwich enzyme immunoassay for TIMP-1 and by an assay for matrix metalloproteinase inhibition. The amount of TIMP-1 in the cytosolic fraction of quiescent Gin-1 cells after stimulation by fetal calf serum increased continuously for 48 hours, whereas that in the nuclear extract showed a maximum at 24 hours (S phase) and significantly decreased thereafter. Gin-1 cells expressed mRNAs for both TIMP-2 and TIMP-3 together with mRNA for TIMP-1. However, neither TIMP-2 nor TIMP-3 proteins seemed to accumulate in the nuclei of Gin-1 cells. These facts strongly suggest that TIMP-1 accumulates specifically in the nuclei of Gin-1 cells in a cell cycle-dependent manner.
This article has been cited by other articles:
|
|
 |
|
  W. G. Stetler-Stevenson Tissue Inhibitors of Metalloproteinases in Cell Signaling: Metalloproteinase-Independent Biological Activities Sci. Signal., July 8, 2008; 1(27): re6 - re6. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Eguchi, S. Kubota, K. Kawata, Y. Mukudai, J. Uehara, T. Ohgawara, S. Ibaragi, A. Sasaki, T. Kuboki, and M. Takigawa Novel Transcription Factor-Like Function of Human Matrix Metalloproteinase 3 Regulating the CTGF/CCN2 Gene Mol. Cell. Biol., April 1, 2008; 28(7): 2391 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jiang, H. Zhu, X. Chen, Y. Peng, J. Wang, F. Liu, S. Shi, B. Fu, Y. Lu, Q. Hong, et al. TIMP-1 Transgenic Mice Recover From Diabetes Induced by Multiple Low-Dose Streptozotocin Diabetes, January 1, 2007; 56(1): 49 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Verstappen and J.W. Von den Hoff Tissue Inhibitors of Metalloproteinases (TIMPs): Their Biological Functions and Involvement in Oral Disease. J. Dent. Res., December 1, 2006; 85(12): 1074 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, X. Chen, Q. Hong, H. Lin, H. Zhu, Q. Liu, J. Wang, Y. Xie, X. Shang, S. Shi, et al. TIMP-1 Promotes Age-Related Renal Fibrosis Through Upregulating ICAM-1 in Human TIMP-1 Transgenic Mice J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2006; 61(11): 1130 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Hockenbery MMPs in Unusual Places Am. J. Pathol., October 1, 2006; 169(4): 1101 - 1103. [Full Text] [PDF]
|
|
|
|
 |
|
 S O Wurtz, A-S Schrohl, N M. Sorensen, U Lademann, I J Christensen, H Mouridsen, and N Brunner Tissue inhibitor of metalloproteinases-1 in breast cancer Endocr. Relat. Cancer, June 1, 2005; 12(2): 215 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Perez-Martinez and D. M. Jaworski Tissue Inhibitor of Metalloproteinase-2 Promotes Neuronal Differentiation by Acting as an Anti-Mitogenic Signal J. Neurosci., May 18, 2005; 25(20): 4917 - 4929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Han and T. J. Smith Induction by IL-1{beta} of Tissue Inhibitor of Metalloproteinase-1 in Human Orbital Fibroblasts: Modulation of Gene Promoter Activity by IL-4 and IFN-{gamma} J. Immunol., March 1, 2005; 174(5): 3072 - 3079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Polyakova, S. Hein, S. Kostin, T. Ziegelhoeffer, and J. Schaper Matrix metalloproteinases and their tissue inhibitors in pressure-overloaded human myocardium during heart failure progression J. Am. Coll. Cardiol., October 19, 2004; 44(8): 1609 - 1618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Visse and H. Nagase Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases: Structure, Function, and Biochemistry Circ. Res., May 2, 2003; 92(8): 827 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-T. Khuth, H. Akaoka, A. Pagenstecher, O. Verlaeten, M.-F. Belin, P. Giraudon, and A. Bernard Morbillivirus Infection of the Mouse Central Nervous System Induces Region-Specific Upregulation of MMPs and TIMPs Correlated to Inflammatory Cytokine Expression J. Virol., September 1, 2001; 75(17): 8268 - 8282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yan and M. A. Moses A Case of Tumor Betrayal : Biphasic Effects of TIMP-1 on Burkitt's Lymphoma Am. J. Pathol., April 1, 2001; 158(4): 1185 - 1190. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nagase and J. F. Woessner Jr. Matrix Metalloproteinases J. Biol. Chem., July 30, 1999; 274(31): 21491 - 21494. [Full Text] [PDF]
|
|
|
|
|
|
B. Price, C. Dennison, H. Tschesche, and E. Elliott Neutrophil Tissue Inhibitor of Matrix Metalloproteinases-1 Occurs in Novel Vesicles That Do Not Fuse with the Phagosome J. Biol. Chem., September 1, 2000; 275(36): 28308 - 28315. [Abstract] [Full Text] [PDF]
|
|
