|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 7 995-1004, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
G Keryer, E Alsat, K Tasken and D Evain-Brion
INSERM Unite 427, Universite Rene Descartes, Faculte des Sciences Pharmaceutiques et Biologiques de Paris, Paris, France.
Human trophoblast cells offer a unique in vitro model for the study of aspects of the dynamic processes occurring during cell fusion and syncytium formation. In the human placenta, mononuclear cytotrophoblasts aggregate and fuse to form a multinucleated syncytiotrophoblast. In vitro, the addition of cyclic AMP analogs, 8-bromo-cyclic-AMP or Sp-8-bromo-cyclic AMPS, promotes syncytiotrophoblast formation, as shown by the disappearance of immunostained E-cadherin and desmoplakin, and increased numbers of nuclei per syncytium. An antagonist of cyclic AMP, Rp-8-bromo-cyclic AMPS, and an inhibitor of the cyclic AMP-dependent protein kinase catalytic subunit, H-89, impair cell fusion. This led us to study the pattern of expression and subcellular localization of cyclic-AMP-dependent protein kinase subunits during syncytium formation. Cytotrophoblasts expressed the RIalpha and RIIalpha regulatory subunits and the Calpha and Cbeta catalytic subunits. RIalpha was down-regulated during syncytium formation. No change in RIIalpha protein levels was observed, but there was a drastic subcellular redistribution. RIIalpha located in the Golgi-centrosomal area of cytotrophoblasts was scattered throughout the cytoplasm of the syncytiotrophoblast. Interestingly, an accumulation of RIIalpha was observed underneath the apical membrane of syncytiotrophoblast in vitro and in situ. This suggests a key role of cyclic AMP-dependent protein kinase type IIalpha during cell fusion and microvilli formation, both of which are essential for the secretory and transfer functions of the syncytiotrophoblast.
This article has been cited by other articles:
|
|
 |
|
  C.-P. Chen, L.-F. Chen, S.-R. Yang, C.-Y. Chen, C.-C. Ko, G.-D. Chang, and H. Chen Functional Characterization of the Human Placental Fusogenic Membrane Protein Syncytin 2 Biol Reprod, November 1, 2008; 79(5): 815 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-W. Chang, H.-C. Chuang, C. Yu, T.-P. Yao, and H. Chen Stimulation of GCMa Transcriptional Activity by Cyclic AMP/Protein Kinase A Signaling Is Attributed to CBP-Mediated Acetylation of GCMa Mol. Cell. Biol., October 1, 2005; 25(19): 8401 - 8414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Johnstone, G. Chan, C. P. Sibley, S. T. Davidge, B. Lowen, and L. J. Guilbert Sphingosine-1-phosphate inhibition of placental trophoblast differentiation through a Gi-coupled receptor response J. Lipid Res., September 1, 2005; 46(9): 1833 - 1839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, Z. Jobbagy, T. H. Bird, M. V. Eiden, and W. B. Anderson Cell Signaling through the Protein Kinases cAMP-dependent Protein Kinase, Protein Kinase C{epsilon}, and RAF-1 Regulates Amphotropic Murine Leukemia Virus Envelope Protein-induced Syncytium Formation J. Biol. Chem., April 29, 2005; 280(17): 16772 - 16783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Prudhomme, G. Oriol, and F. Mallet A Retroviral Promoter and a Cellular Enhancer Define a Bipartite Element Which Controls env ERVWE1 Placental Expression J. Virol., November 15, 2004; 78(22): 12157 - 12168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.J.G. Potgens, S. Drewlo, M. Kokozidou, and P. Kaufmann Syncytin: the major regulator of trophoblast fusion? Recent developments and hypotheses on its action Hum. Reprod. Update, November 1, 2004; 10(6): 487 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Cheng, B. J. Aronow, S. Hossain, B. Trapnell, S. Kong, and S. Handwerger Critical role for transcription factor AP-2{alpha} in human trophoblast differentiation Physiol Genomics, June 17, 2004; 18(1): 99 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Knofler, L. Saleh, S. Bauer, B. Galos, H. Rotheneder, P. Husslein, and H. Helmer Transcriptional Regulation of the Human Chorionic Gonadotropin {beta} Gene during Villous Trophoblast Differentiation Endocrinology, April 1, 2004; 145(4): 1685 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Frendo, L. Cronier, G. Bertin, J. Guibourdenche, M. Vidaud, D. Evain-Brion, and A. Malassine Involvement of connexin 43 in human trophoblast cell fusion and differentiation J. Cell Sci., August 15, 2003; 116(16): 3413 - 3421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Frendo, D. Olivier, V. Cheynet, J.-L. Blond, O. Bouton, M. Vidaud, M. Rabreau, D. Evain-Brion, and F. Mallet Direct Involvement of HERV-W Env Glycoprotein in Human Trophoblast Cell Fusion and Differentiation Mol. Cell. Biol., May 15, 2003; 23(10): 3566 - 3574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yang, Z. M. Lei, and Ch. V. Rao The Central Role of Human Chorionic Gonadotropin in the Formation of Human Placental Syncytium Endocrinology, March 1, 2003; 144(3): 1108 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Debieve and K. Thomas Control of the human inhibin {alpha} chain promoter in cytotrophoblast cells differentiating into syncytium Mol. Hum. Reprod., March 1, 2002; 8(3): 262 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tarrade, K. Schoonjans, J. Guibourdenche, J. M. Bidart, M. Vidaud, J. Auwerx, C. Rochette-Egly, and D. Evain-Brion PPAR{gamma}/RXR{alpha} Heterodimers Are Involved in Human CG{beta} Synthesis and Human Trophoblast Differentiation Endocrinology, October 1, 2001; 142(10): 4504 - 4514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Frendo, P. Therond, T. Bird, N. Massin, F. Muller, J. Guibourdenche, D. Luton, M. Vidaud, W. B. Anderson, and D. Evain-Brion Overexpression of Copper Zinc Superoxide Dismutase Impairs Human Trophoblast Cell Fusion and Differentiation Endocrinology, August 1, 2001; 142(8): 3638 - 3648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Bidart, L. Lacroix, D. Evain-Brion, B. Caillou, V. Lazar, R. Frydman, D. Bellet, S. Filetti, and M. Schlumberger Expression of Na+/I- Symporter and Pendred Syndrome Genes in Trophoblast Cells J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4367 - 4372. [Abstract] [Full Text]
|
|
|
|
|
|
J. L. Frendo, M. Vidaud, J. Guibourdenche, D. Luton, F. Muller, D. Bellet, Y. Giovagrandi, A. Tarrade, D. Porquet, P. Blot, et al. Defect of Villous Cytotrophoblast Differentiation into Syncytiotrophoblast in Down's Syndrome J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3700 - 3707. [Abstract] [Full Text]
|
|
|
|
|
|
W. T. Schaiff, M. G. Carlson, S. D. Smith, R. Levy, D. M. Nelson, and Y. Sadovsky Peroxisome Proliferator-Activated Receptor-{gamma} Modulates Differentiation of Human Trophoblast in a Ligand-Specific Manner J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3874 - 3881. [Abstract] [Full Text]
|
|
|
|
|
|
J. Guibourdenche, A. Tarrade, I. Laurendeau, C. Rochette-Egly, P. Chambon, M. Vidaud, and D. Evain-Brion Retinoids Stimulate Leptin Synthesis and Secretion in Human Syncytiotrophoblast J. Clin. Endocrinol. Metab., July 1, 2000; 85(7): 2550 - 2555. [Abstract] [Full Text]
|
|
|
|
|
|
M. Knofler, L. Saleh, H. Strohmer, P. Husslein, and M. F. Wolschek Cyclic AMP- and differentiation-dependent regulation of the proximal {alpha}HCG gene promoter in term villous trophoblasts Mol. Hum. Reprod., June 1, 1999; 5(6): 573 - 580. [Abstract] [Full Text] [PDF]
|
|
