|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 6 825-832, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
U Vogel, K Sandvig and B van Deurs
Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, DK-2200 Copenhagen N, Denmark.
We have studied caveolin-1 expression and the frequency and distribution of typical invaginated caveolae as they are identified by electron microscopy in the polarized epithelial cell lines MDCK II and Caco-2. In wild-type MDCK II cells caveolin expression is high and more than 400 caveolae/mm filter were observed at the basolateral membrane. No caveolae were found at the apical surface. By contrast, wild-type Caco-2 cells do not express caveolin-1 and have extremely few, if any caveolae. Caco-2 cells were stably transfected with the gene for caveolin-1 in order to investigate if the formation of caveolae is polarized also in these cells. We have isolated Caco-2 clones expressing different levels of caveolin-1, where the level of expression varies from 10-100% of the endogenous level in MDCK II cells. Caveolin-1 expression in Caco-2 cells gives rise to a marked immunofluorescense labeling mainly at the lateral plasma membrane. By electron microscopy an increase from less than 4 caveolae/mm filter in wild-type Caco-2 cells to 21-76 caveolae/mm filter in Caco-2 clones transfected with caveolin-1 was revealed and these caveolae were exclusively localized to the basolateral membrane. Thus expression of heterologous caveolin-1 in Caco-2 cells leads to polarized formation of caveolae, but there is a lack of correlation between the amount of caveolin expressed in the cells and the number of caveolae, suggesting that factors in addition to caveolin are required for generation of caveolae.
This article has been cited by other articles:
|
|
 |
|
  S. A. Predescu, D. N. Predescu, and A. B. Malik Molecular determinants of endothelial transcytosis and their role in endothelial permeability Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L823 - L842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shmuel, E. Nodel-Berner, T. Hyman, A. Rouvinski, and Y. Altschuler Caveolin 2 Regulates Endocytosis and Trafficking of the M1 Muscarinic Receptor in MDCK Epithelial Cells Mol. Biol. Cell, May 1, 2007; 18(5): 1570 - 1585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Martin Caveolae and cell swelling. Focus on "Stimulation by caveolin-1 of the hypotonicity-induced release of taurine and ATP at basolateral, but not apical, membrane of Caco-2 cells" Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1273 - C1274. [Full Text] [PDF]
|
|
|
|
|
|
N. Ullrich, A. Caplanusi, B. Brone, D. Hermans, E. Lariviere, B. Nilius, W. Van Driessche, and J. Eggermont Stimulation by caveolin-1 of the hypotonicity-induced release of taurine and ATP at basolateral, but not apical, membrane of Caco-2 cells Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1287 - C1296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Parton, M. Hanzal-Bayer, and J. F. Hancock Biogenesis of caveolae: a structural model for caveolin-induced domain formation. J. Cell Sci., March 1, 2006; 119(Pt 5): 787 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Mora, V. L. Bonilha, B.-C. Shin, J. Hu, L. Cohen-Gould, D. Bok, and E. Rodriguez-Boulan Bipolar assembly of caveolae in retinal pigment epithelium Am J Physiol Cell Physiol, March 1, 2006; 290(3): C832 - C843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Papanikolaou, A. Papafotika, C. Murphy, T. Papamarcaki, O. Tsolas, M. Drab, T. V. Kurzchalia, M. Kasper, and S. Christoforidis Cholesterol-dependent Lipid Assemblies Regulate the Activity of the Ecto-nucleotidase CD39 J. Biol. Chem., July 15, 2005; 280(28): 26406 - 26414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lupu, X. Hu, and F. Lupu Caveolin-1 Enhances Tissue Factor Pathway Inhibitor Exposure and Function on the Cell Surface J. Biol. Chem., June 10, 2005; 280(23): 22308 - 22317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Bauer, J. Yu, Y. Chen, R. Hickey, P. N. Bernatchez, R. Looft-Wilson, Y. Huang, F. Giordano, R. V. Stan, and W. C. Sessa Endothelial-specific expression of caveolin-1 impairs microvascular permeability and angiogenesis PNAS, January 4, 2005; 102(1): 204 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mehta, J. Bhattacharya, M. A. Matthay, and A. B. Malik Integrated control of lung fluid balance Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1081 - L1090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kansau, C. Berger, M. Hospital, R. Amsellem, V. Nicolas, A. L. Servin, and M.-F. Bernet-Camard Zipper-Like Internalization of Dr-Positive Escherichia coli by Epithelial Cells Is Preceded by an Adhesin-Induced Mobilization of Raft-Associated Molecules in the Initial Step of Adhesion Infect. Immun., July 1, 2004; 72(7): 3733 - 3742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Duncan, G. Li, J.-S. Shin, J. L. Carson, and S. N. Abraham Bacterial Penetration of Bladder Epithelium through Lipid Rafts J. Biol. Chem., April 30, 2004; 279(18): 18944 - 18951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. V. Burgos, C. Klattenhoff, E. de la Fuente, A. Rigotti, and A. Gonzalez Cholesterol depletion induces PKA-mediated basolateral-to-apical transcytosis of the scavenger receptor class B type I in MDCK cells PNAS, March 16, 2004; 101(11): 3845 - 3850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pang, P. U. Le, and I. R. Nabi Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus J. Cell Sci., March 15, 2004; 117(8): 1421 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hnasko and M. P. Lisanti The Biology of Caveolae: Lessons from Caveolin Knockout Mice and Implications for Human Disease Mol. Interv., December 1, 2003; 3(8): 445 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Capozza, T. M. Williams, W. Schubert, S. McClain, B. Bouzahzah, F. Sotgia, and M. P. Lisanti Absence of Caveolin-1 Sensitizes Mouse Skin to Carcinogen-Induced Epidermal Hyperplasia and Tumor Formation Am. J. Pathol., June 1, 2003; 162(6): 2029 - 2039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sowa, M. Pypaert, D. Fulton, and W. C. Sessa The phosphorylation of caveolin-2 on serines 23 and 36 modulates caveolin-1-dependent caveolae formation PNAS, May 27, 2003; 100(11): 6511 - 6516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Torgersen, G. Skretting, B. van Deurs, and K. Sandvig Internalization of cholera toxin by different endocytic mechanisms J. Cell Sci., March 12, 2002; 114(20): 3737 - 3747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Breuza, S. Corby, J.-P. Arsanto, M.-H. Delgrossi, P. Scheiffele, and A. Le Bivic The scaffolding domain of caveolin 2 is responsible for its Golgi localization in Caco-2 cells J. Cell Sci., January 12, 2002; 115(23): 4457 - 4467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Simons and E. Ikonen How Cells Handle Cholesterol Science, December 1, 2000; 290(5497): 1721 - 1726. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Badizadegan, B. L. Dickinson, H. E. Wheeler, R. S. Blumberg, R. K. Holmes, and W. I. Lencer Heterogeneity of detergent-insoluble membranes from human intestine containing caveolin-1 and ganglioside GM1 Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G895 - G904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Verkade, T. Harder, F. Lafont, and K. Simons Induction of Caveolae in the Apical Plasma Membrane of Madin-Darby Canine Kidney Cells J. Cell Biol., February 21, 2000; 148(4): 727 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Brown Targeting of membrane transporters in renal epithelia: when cell biology meets physiology Am J Physiol Renal Physiol, February 1, 2000; 278(2): F192 - F201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Fujimoto, H Kogo, R Nomura, and T Une Isoforms of caveolin-1 and caveolar structure J. Cell Sci., January 10, 2000; 113(19): 3509 - 3517. [Abstract] [PDF]
|
|
|
|
|
|
M. Stahlhut and B. van Deurs Identification of Filamin as a Novel Ligand for Caveolin-1: Evidence for the Organization of Caveolin-1-associated Membrane Domains by the Actin Cytoskeleton Mol. Biol. Cell, January 1, 2000; 11(1): 325 - 337. [Abstract] [Full Text]
|
|
|
|
|
|
R. Mora, V. L. Bonilha, A. Marmorstein, P. E. Scherer, D. Brown, M. P. Lisanti, and E. Rodriguez-Boulan Caveolin-2 Localizes to the Golgi Complex but Redistributes to Plasma Membrane, Caveolae, and Rafts when Co-expressed with Caveolin-1 J. Biol. Chem., September 3, 1999; 274(36): 25708 - 25717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Hansen, B. van Deurs, L. C. Petersen, and L. V. M. Rao Discordant Expression of Tissue Factor and Its Activity in Polarized Epithelial Cells. Asymmetry in Anionic Phospholipid Availability as a Possible Explanation Blood, September 1, 1999; 94(5): 1657 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Maekawa, C. Sato, K. Kitajima, N. Funatsu, H. Kumanogoh, and Y. Sokawa Cholesterol-dependent Localization of NAP-22 on a Neuronal Membrane Microdomain (Raft) J. Biol. Chem., July 23, 1999; 274(30): 21369 - 21374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Bradbury, J. A. Clark, S. C. Watkins, C. C. Widnell, H. S. Smith IV, and R. J. Bridges Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator Am J Physiol Lung Cell Mol Physiol, April 1, 1999; 276(4): L659 - L668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Rodal, G. Skretting, O. Garred, F. Vilhardt, B. van Deurs, and K. Sandvig Extraction of Cholesterol with Methyl-beta -Cyclodextrin Perturbs Formation of Clathrin-coated Endocytic Vesicles Mol. Biol. Cell, April 1, 1999; 10(4): 961 - 974. [Abstract] [Full Text]
|
|
|
|
|
|
F. Lafont, P. Verkade, T. Galli, C. Wimmer, D. Louvard, and K. Simons Raft association of SNAP receptors acting in apical trafficking in Madin-Darby canine kidney cells PNAS, March 30, 1999; 96(7): 3734 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Skretting, M. Torgersen, B van Deurs, and K Sandvig Endocytic mechanisms responsible for uptake of GPI-linked diphtheria toxin receptor J. Cell Sci., January 11, 1999; 112(22): 3899 - 3909. [Abstract] [PDF]
|
|
|
|
|
|
F. Vilhardt, M. Nielsen, K. Sandvig, and B. van Deurs Urokinase-Type Plasminogen Activator Receptor Is Internalized by Different Mechanisms in Polarized and Nonpolarized Madin-Darby Canine Kidney Epithelial Cells Mol. Biol. Cell, January 1, 1999; 10(1): 179 - 195. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. J. Field, E. Born, S. Murthy, and S. N. Mathur Caveolin is present in intestinal cells: role in cholesterol trafficking? J. Lipid Res., October 1, 1998; 39(10): 1938 - 1950. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Sanders and M. D. Basson Collagen IV-dependent ERK Activation in Human Caco-2 Intestinal Epithelial Cells Requires Focal Adhesion Kinase J. Biol. Chem., November 22, 2000; 275(48): 38040 - 38047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Abrami, M. Fivaz, T. Kobayashi, T. Kinoshita, R. G. Parton, and F. G. van der Goot Cross-talk between Caveolae and Glycosylphosphatidylinositol-rich Domains J. Biol. Chem., August 10, 2001; 276(33): 30729 - 30736. [Abstract] [Full Text] [PDF]
|
|
