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Journal of Cell Science, Vol 111, Issue 6 667-673, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
S Hisahara, H Kanuka, S Shoji, S Yoshikawa, H Okano and M Miura
Department of Neuroanatomy, Biomedical Research Center, Osaka University Medical School, Osaka, Japan.
ced-9, a member of the bcl-2 gene family in Caenorhabditis elegans plays a central roles in preventing cell death in worms. Overexpression of human bcl-2 can partially prevent cell death in C. elegans. However, it remains to be elucidated whether ced-9 can regulate cell death when expressed in other organisms. We demonstrated that the CED-9 protein is co-localized with BCL-2 in COS cells and Drosophila Schneider's L2 (SL2) cells, suggesting that the site of CED-9 action is located to specific cytoplasmic compartments. Overexpression of ced-9 only poorly protected cells from the death induced by ced-3 in HeLa cells, but ced-9 significantly reduced the cell death induced by ced-3 in Drosophila SL2 cells. Furthermore, apoptosis of SL2 cells that was induced by a Drosophila cell-death gene, reaper, was shown to be partially prevented by ced-9, bcl-2 and bcl-xL. These results suggest that the signaling pathway that is required for the anti-apoptotic function of bcl-2 family members, including ced-9, is conserved in Drosophila cells. In addition, SL2 cells provide a unique systems for dissecting the main machinery of cell death.
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H. Kanda, T. Igaki, H. Kanuka, T. Yagi, and M. Miura Wengen, a Member of the Drosophila Tumor Necrosis Factor Receptor Superfamily, Is Required for Eiger Signaling J. Biol. Chem., August 2, 2002; 277(32): 28372 - 28375. [Abstract] [Full Text] [PDF]
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 T. Igaki, H. Kanuka, N. Inohara, K. Sawamoto, G. Nunez, H. Okano, and M. Miura Drob-1, a Drosophila member of the Bcl-2/CED-9 family that promotes cell death PNAS, January 18, 2000; 97(2): 662 - 667. [Abstract] [Full Text] [PDF]
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