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Journal of Cell Science, Vol 111, Issue 15 2209-2215, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

Release of 'caged' cytosolic Ca2+ triggers rapid spreading of human neutrophils adherent via integrin engagement

EJ Pettit and MB Hallett
Molecular Signalling Group, Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, UK.

The role of the transient rise in cytosolic free Ca2+ which occurs during neutrophil adhesion and cell spreading is unclear. In order to establish whether such a Ca2+ signal triggers neutrophil shape change, neutrophils co-loaded with fluo3 and Nitr5 ('caged' Ca2+) were used with rapid-time confocal laser scanning microscopy. Here we show that the photolytic generation of a Ca2+ rise in neutrophils which were adherent to an integrin-engaging surface, triggered a rapid change in cell morphology, with increases in cell diameter of approximately 175% occurring within 90 seconds of the Ca2+ signal. In non-adhered neutrophils or neutrophils on plain glass, no acceleration of the rate of spreading occurring in response to the release of 'caged Ca2+' could be demonstrated. It was concluded that although a rise in cytosolic free Ca2+ was not the sole trigger for neutrophil shape change, with other signals generated by integrin engagement, a rise in cytosolic free Ca2+ accelerated the rate of neutrophil spreading.


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© The Company of Biologists Ltd 1998