|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 111, Issue 15 2189-2195, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
X Huang, J Wu, S Spong and D Sheppard
Lung Biology Center, Cardiovascular Research Institute, Center for Occupational and Environmental Health and Department of Medicine, University of California, San Francisco, USA.
The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation. However, because of the lack of tools to specifically inhibit the function of this integrin, little is known about its effects on cell behavior. To directly examine the role of this integrin in cell migration, we used keratinocytes derived from wild-type mice or mice expressing a null mutation in the beta6 subunit (beta6-/-) to perform migration assays in vitro. Migration on the known alphavbeta6 ligand, fibronectin was reduced in keratinocytes from beta6-/- mice. Interestingly, keratinocytes from beta6-/- mice also demonstrated markedly reduced migration on vitronectin, a protein not previously known to be a ligand for alphavbeta6. An anti-alphavbeta6 monoclonal antibody 10D5, generated by immunization of beta6-/- mice with murine keratinocytes, inhibited adhesion and migration of wild-type keratinocyte on both vitronectin and fibronectin to levels similar to those seen with keratinocytes from beta6-/- mice. alphavbeta6-mediated migration on both ligands was dramatically augmented by treatment with phorbol myrisate acetate (PMA) or with hepatocyte growth factor, and augmentation of migration by either stimulus could be abolished by the PKC inhibitor GF109203X, suggesting a critical role for PKC in enhancement of alphavbeta6-mediated cell migration.
This article has been cited by other articles:
|
|
 |
|
  F. Meng, X. Cheng, L. Yang, N. Hou, X. Yang, and A. Meng Accelerated re-epithelialization in Dpr2-deficient mice is associated with enhanced response to TGF{beta} signaling J. Cell Sci., September 1, 2008; 121(17): 2904 - 2912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ghannad, D. Nica, M. I. Garcia Fulle, D. Grenier, E. E. Putnins, S. Johnston, A. Eslami, L. Koivisto, G. Jiang, M. D. McKee, et al. Absence of {alpha}v{beta}6 Integrin Is Linked to Initiation and Progression of Periodontal Disease Am. J. Pathol., May 1, 2008; 172(5): 1271 - 1286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tabata, H. Kawakatsu, E. Maidji, T. Sakai, K. Sakai, J. Fang-Hoover, M. Aiba, D. Sheppard, and L. Pereira Induction of an Epithelial Integrin {alpha}v{beta}6 in Human Cytomegalovirus-Infected Endothelial Cells Leads to Activation of Transforming Growth Factor-{beta}1 and Increased Collagen Production Am. J. Pathol., April 1, 2008; 172(4): 1127 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Koria and S. T. Andreadis KGF promotes integrin {alpha}5 expression through CCAAT/enhancer-binding protein-beta Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1020 - C1031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Azare, K. Leslie, H. Al-Ahmadie, W. Gerald, P. H. Weinreb, S. M. Violette, and J. Bromberg Constitutively Activated Stat3 Induces Tumorigenesis and Enhances Cell Motility of Prostate Epithelial Cells through Integrin {beta}6 Mol. Cell. Biol., June 15, 2007; 27(12): 4444 - 4453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jovanovic, J. Takagi, L. Choulier, N. G. A. Abrescia, D. I. Stuart, P. A. van der Merwe, H. J. Mardon, and P. A. Handford {alpha}Vbeta6 Is a Novel Receptor for Human Fibrillin-1: COMPARATIVE STUDIES OF MOLECULAR DETERMINANTS UNDERLYING INTEGRIN-RGD AFFINITY AND SPECIFICITY J. Biol. Chem., March 2, 2007; 282(9): 6743 - 6751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hahm, M. E. Lukashev, Y. Luo, W. J. Yang, B. M. Dolinski, P. H. Weinreb, K. J. Simon, L. Chun Wang, D. R. Leone, R. R. Lobb, et al. {alpha}v{beta}6 Integrin Regulates Renal Fibrosis and Inflammation in Alport Mouse Am. J. Pathol., January 1, 2007; 170(1): 110 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Brown, S. M. McAleese, E. M. Thornton, J. A. Pate, A. Schock, A. I. Macrae, P. R. Scott, H. R.P. Miller, and D. D.S. Collie Integrin-{alpha}v{beta}6, a Putative Receptor for Foot-and-Mouth Disease Virus, Is Constitutively Expressed in Ruminant Airways J. Histochem. Cytochem., July 1, 2006; 54(7): 807 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fontana, Y. Chen, P. Prijatelj, T. Sakai, R. Fassler, L. Y. Sakai, and D. B. Rifkin Fibronectin is required for integrin {alpha}v{beta}6-mediated activation of latent TGF-{beta} complexes containing LTBP-1 FASEB J, November 1, 2005; 19(13): 1798 - 1808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Roux, H. Kawakatsu, B. Gartland, M. Pespeni, D. Sheppard, M. A. Matthay, C. M. Canessa, and J.-F. Pittet Interleukin-1{beta} Decreases Expression of the Epithelial Sodium Channel {alpha}-Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway J. Biol. Chem., May 13, 2005; 280(19): 18579 - 18589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ambesi, R. M. Klein, K. M. Pumiglia, and P. J. McKeown-Longo Anastellin, a Fragment of the First Type III Repeat of Fibronectin, Inhibits Extracellular Signal-Regulated Kinase and Causes G1 Arrest in Human Microvessel Endothelial Cells Cancer Res., January 1, 2005; 65(1): 148 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Annes, Y. Chen, J. S. Munger, and D. B. Rifkin Integrin {alpha}V{beta}6-mediated activation of latent TGF-{beta} requires the latent TGF-{beta} binding protein-1 J. Cell Biol., June 7, 2004; 165(5): 723 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Jackson, S. Clark, S. Berryman, A. Burman, S. Cambier, D. Mu, S. Nishimura, and A. M. Q. King Integrin {alpha}v{beta}8 Functions as a Receptor for Foot-and-Mouth Disease Virus: Role of the {beta}-Chain Cytodomain in Integrin-Mediated Infection J. Virol., May 1, 2004; 78(9): 4533 - 4540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. H. Weinreb, K. J. Simon, P. Rayhorn, W. J. Yang, D. R. Leone, B. M. Dolinski, B. R. Pearse, Y. Yokota, H. Kawakatsu, A. Atakilit, et al. Function-blocking Integrin {alpha}v{beta}6 Monoclonal Antibodies: DISTINCT LIGAND-MIMETIC AND NONLIGAND-MIMETIC CLASSES J. Biol. Chem., April 23, 2004; 279(17): 17875 - 17887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. SHEPPARD Functions of Pulmonary Epithelial Integrins: From Development to Disease Physiol Rev, July 1, 2003; 83(3): 673 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Manes, D.-Q. Zheng, S. Tognin, A. S. Woodard, P. C. Marchisio, and L. R. Languino {alpha}v{beta}3 integrin expression up-regulates cdc2, which modulates cell migration J. Cell Biol., May 26, 2003; 161(4): 817 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, K. Chen, Y. Tu, A. Velyvis, Y. Yang, J. Qin, and C. Wu Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites J. Cell Sci., March 14, 2003; 115(24): 4777 - 4786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Knight, S. H. Wright, J. K. Brown, X. Huang, D. Sheppard, and H. R. P. Miller Enteric Expression of the Integrin {alpha}v{beta}6 Is Essential for Nematode-Induced Mucosal Mast Cell Hyperplasia and Expression of the Granule Chymase, Mouse Mast Cell Protease-1 Am. J. Pathol., September 1, 2002; 161(3): 771 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Grose, C. Hutter, W. Bloch, I. Thorey, F. M. Watt, R. Fassler, C. Brakebusch, and S. Werner A crucial role of {beta}1 integrins for keratinocyte migration in vitro and during cutaneous wound repair Development, January 5, 2002; 129(9): 2303 - 2315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Ljubimov, M. Saghizadeh, R. Pytela, D. Sheppard, and M. C. Kenney Increased Expression of Tenascin-C-binding Epithelial Integrins in Human Bullous Keratopathy Corneas J. Histochem. Cytochem., November 1, 2001; 49(11): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Friedl, S. Borgmann, and E.-B. Brocker Amoeboid leukocyte crawling through extracellular matrix: lessons from the Dictyostelium paradigm of cell movement J. Leukoc. Biol., October 1, 2001; 70(4): 491 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sheppard Integrin-Mediated Activation of Transforming Growth Factor-{beta}1 in Pulmonary Fibrosis Chest, July 1, 2001; 120(2007): 49S - 53S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Miller, W. Blakemore, D. Sheppard, A. Atakilit, A. M. Q. King, and T. Jackson Role of the Cytoplasmic Domain of the {beta}-Subunit of Integrin {alpha}v{beta}6 in Infection by Foot-and-Mouth Disease Virus J. Virol., May 1, 2001; 75(9): 4158 - 4164. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Miller, N. L. Barnett, D. Sheppard, and D. M. Hyde Expression of the {beta}6 Integrin Subunit Is Associated with Sites of Neutrophil Influx in Lung Epithelium J. Histochem. Cytochem., January 1, 2001; 49(1): 41 - 48. [Abstract] [Full Text]
|
|
|
|
|
|
S. Cambier, D.-z. Mu, D. O’Connell, K. Boylen, W. Travis, W.-h. Liu, V. C. Broaddus, and S. L. Nishimura A Role for the Integrin {{alpha}}v{beta}8 in the Negative Regulation of Epithelial Cell Growth Cancer Res., December 1, 2000; 60(24): 7084 - 7093. [Abstract] [Full Text]
|
|
|
|
|
|
T. Jackson, D. Sheppard, M. Denyer, W. Blakemore, and A. M. Q. King The Epithelial Integrin alpha vbeta 6 Is a Receptor for Foot-and-Mouth Disease Virus J. Virol., June 1, 2000; 74(11): 4949 - 4956. [Abstract] [Full Text]
|
|
|
|
|
|
X. Huang, M. Griffiths, J. Wu, R. V. Farese Jr., and D. Sheppard Normal Development, Wound Healing, and Adenovirus Susceptibility in beta 5-Deficient Mice Mol. Cell. Biol., February 1, 2000; 20(3): 755 - 759. [Abstract] [Full Text]
|
|
|
|
|
|
A. Erdreich-Epstein, H. Shimada, S. Groshen, M. Liu, L. S. Metelitsa, K. S. Kim, M. F. Stins, R. C. Seeger, and D. L. Durden Integrins {{alpha}}v{beta}3 and {{alpha}}v{beta}5 Are Expressed by Endothelium of High-Risk Neuroblastoma and Their Inhibition Is Associated with Increased Endogenous Ceramide Cancer Res., February 1, 2000; 60(3): 712 - 721. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. S. Spirin, A. V. Ljubimov, R. Castellon, O. Wiedoeft, M. Marano, D. Sheppard, M. C. Kenney, and D. J. Brown Analysis of Gene Expression in Human Bullous Keratopathy Corneas Containing Limiting Amounts of RNA Invest. Ophthalmol. Vis. Sci., December 1, 1999; 40(13): 3108 - 3115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Miranti, S. Ohno, and J. S. Brugge Protein Kinase C Regulates Integrin-induced Activation of the Extracellular Regulated Kinase Pathway Upstream of Shc J. Biol. Chem., April 9, 1999; 274(15): 10571 - 10581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Milner, X Huang, J Wu, S Nishimura, R Pytela, D Sheppard, and C ffrench-Constant Distinct roles for astrocyte alphavbeta5 and alphavbeta8 integrins in adhesion and migration J. Cell Sci., January 12, 1999; 112(23): 4271 - 4279. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Sano, M. Kira, S. Takagi, K. Yoshikawa, J. Takeda, and S. Itami Two distinct signaling pathways in hair cycle induction: Stat3-dependent and -independent pathways PNAS, December 5, 2000; 97(25): 13824 - 13829. [Abstract] [Full Text] [PDF]
|
|
