|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 22 2781-2794, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
GK Ojakian, WJ Nelson and KA Beck
Department of Anatomy and Cell Biology, State University of New York Health Science Center, Brooklyn 11203, USA.
In open monolayers of epithelial cells grown in vitro, the apical membrane domain forms on the free cell surface that faces the culture medium. However, in vivo, the apical lumenal compartment arises within groups of cells that do not have a free cell surface. We designed in vitro culture conditions, using small colonies of MDCK cells overlaid with collagen, in which formation of the apical membrane must occur de novo by remodeling existing membrane domains that are contacted by other cells or extracellular matrix. Within 12 hours of collagen overlay, the apical membrane glycoprotein gp135 is removed from the free cell surface, while lateral membrane proteins (e.g. Na+,K+-ATPase) remain at sites of cell-cell contacts. Subsequently, lumenal structures, containing gp135 and the apically secreted protein gp81, formed within these cell-cell contacts. Na+,K+-ATPase, adherens junction (E-cadherin, alpha- and beta-catenins) and tight junction (ZO-1) proteins were localized on the lateral membrane adjacent to, but excluded from the gp135-positive lumenal compartment. Therefore, each lumen represents a newly formed apical compartment on the lateral membrane. The Golgi complex (alpha-mannosidase II and Golgi beta-spectrin), centrosomes (gamma-tubulin) and microtubules reorient to a cytoplasmic position adjacent to the newly-forming apical lumenal compartments. Significantly, addition of colchicine, nocodazole or brefeldin A inhibits apical lumen formation. These results demonstrate that simple epithelial cells form an apical lumenal compartment de novo through initial intermixing, and then sorting of apical and basal-lateral membrane proteins at sites of cell-cell contacts. In addition, apical lumen formation requires an intact microtubule network, microtubule-dependent reorientation of the Golgi complex and secretory apparatus, and fully functional protein delivery from the Golgi complex to the forming apical cell surface.
This article has been cited by other articles:
|
|
 |
|
  H. Herrema, D. Czajkowska, D. Theard, J. M. van der Wouden, D. Kalicharan, B. Zolghadr, D. Hoekstra, and S. C.D. van IJzendoorn Rho Kinase, Myosin-II, and p42/44 MAPK Control Extracellular Matrix-mediated Apical Bile Canalicular Lumen Morphogenesis in HepG2 Cells Mol. Biol. Cell, July 1, 2006; 17(7): 3291 - 3303. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamanaka, Y. Horikoshi, N. Izumi, A. Suzuki, K. Mizuno, and S. Ohno Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity J. Cell Sci., May 15, 2006; 119(10): 2107 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Eisen, S. Walid, D. R. Ratcliffe, and G. K. Ojakian Regulation of epithelial tubule formation by Rho family GTPases Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1297 - C1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Cheng, Y.-Y. Lin, C.-Y. Yu, J.-Y. Chen, K.-F. Shen, W.-L. Lin, H.-K. Liao, Y.-J. Chen, C.-H. Liu, V. F. Pang, et al. Molecular Identification of Canine Podocalyxin-Like Protein 1 as a Renal Tubulogenic Regulator J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1612 - 1622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Khoury, M. A. Naujokas, D. Zuo, V. Sangwan, M. M. Frigault, S. Petkiewicz, D. L. Dankort, W. J. Muller, and M. Park HGF Converts ErbB2/Neu Epithelial Morphogenesis to Cell Invasion Mol. Biol. Cell, February 1, 2005; 16(2): 550 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Meder, A. Shevchenko, K. Simons, and J. Fullekrug Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells J. Cell Biol., January 17, 2005; 168(2): 303 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Sheng, J. A. Lewis, and W. J. Chirico Nuclear and Nucleolar Localization of 18-kDa Fibroblast Growth Factor-2 Is Controlled by C-terminal Signals J. Biol. Chem., September 17, 2004; 279(38): 40153 - 40160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eisen, D. R. Ratcliffe, and G. K. Ojakian Modulation of epithelial tubule formation by Rho kinase Am J Physiol Cell Physiol, April 1, 2004; 286(4): C857 - C866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cohen, P. J. Brennwald, E. Rodriguez-Boulan, and A. Musch Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton J. Cell Biol., March 1, 2004; 164(5): 717 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ojakian, D. Ratcliffe, and R Schwimmer Integrin regulation of cell-cell adhesion during epithelial tubule formation J. Cell Sci., January 3, 2001; 114(5): 941 - 952. [Abstract] [PDF]
|
|
|
|
 |
|
 C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]
|
|
