spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caspari, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caspari, T.

Journal of Cell Science, Vol 110, Issue 20 2599-2608, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Onset of gluconate-H+ symport in Schizosaccharomyces pombe is regulated by the kinases Wis1 and Pka1, and requires the gti1+ gene product

T Caspari
MRC Cell Mutation Unit, University of Sussex, Brighton, UK. T.Caspari@sussex.ac.uk

In the fission yeast Schizosaccharomyces pombe, glucose represses onset of gluconate-H+ symport and inhibits transiently the activity of the symport protein. Wild-type cells harvested from high glucose medium take up gluconate very slowly and the rate of uptake is increased 150-fold in response to glucose starvation. Here it is shown that an intact cAMP cascade is necessary to prevent premature onset in the presence of high glucose concentrations. Cells which have lost either adenylate cyclase (Cyr1) or cAMP-dependent protein kinase (Pka1) transport gluconate up to 60-fold faster than wild-type cells when harvested from high glucose medium. Moreover, inactivation of the stress-sensing Wis1-Sty1 MAP kinase pathway, by loss of Wis1 MAP kinase kinase, diminishes 10-fold the onset of gluconate uptake in response to starvation. A mutant was identified showing a comparable phenotype. By complementation, the gti1+ (gluconate transport inducer 1) gene has been isolated. Disruption of gti1 reduces starvation-induced onset by a similar factor to that observed in wis1 delta cells. Cells over-expressing gti1+ induce gluconate uptake much faster resulting in a threefold higher uptake rate, although gti1+ does not code for the gluconate transport protein. In contrast to the repression of onset, transient downregulation of the gluconate symporter is independent of Pka1 activity and requires ongoing glucose influx. Addition of glucose to starved cyr1 delta cells reduces uptake 9-fold, whereas starved pka1 delta cells, which are able to synthesise cAMP, respond with a 60-fold decrease in transport.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Q. Nguyen and A. Sil
Temperature-induced switch to the pathogenic yeast form of Histoplasma capsulatum requires Ryp1, a conserved transcriptional regulator
PNAS, March 25, 2008; 105(12): 4880 - 4885.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. Srikantha, A. R. Borneman, K. J. Daniels, C. Pujol, W. Wu, M. R. Seringhaus, M. Gerstein, S. Yi, M. Snyder, and D. R. Soll
TOS9 Regulates White-Opaque Switching in Candida albicans
Eukaryot. Cell, October 1, 2006; 5(10): 1674 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. E. Zordan, D. J. Galgoczy, and A. D. Johnson
From the Cover: Epigenetic properties of white-opaque switching in Candida albicans are based on a self-sustaining transcriptional feedback loop
PNAS, August 22, 2006; 103(34): 12807 - 12812.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Huang, H. Wang, S. Chou, X. Nie, J. Chen, and H. Liu
From the Cover: Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans
PNAS, August 22, 2006; 103(34): 12813 - 12818.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Y. Tang and M. McLeod
In Vivo Activation of Protein Kinase A in Schizosaccharomyces pombe Requires Threonine Phosphorylation at Its Activation Loop and Is Dependent on PDK1
Genetics, December 1, 2004; 168(4): 1843 - 1853.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. Stiefel, L. Wang, D. A. Kelly, R. T. K. Janoo, J. Seitz, S. K. Whitehall, and C. S. Hoffman
Suppressors of an Adenylate Cyclase Deletion in the Fission Yeast Schizosaccharomyces pombe
Eukaryot. Cell, June 1, 2004; 3(3): 610 - 619.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
V. Paredes, A. Franco, T. Soto, J. Vicente-Soler, M. Gacto, and J. Cansado
Different roles for the stress-activated protein kinase pathway in the regulation of trehalose metabolism in Schizosaccharomyces pombe
Microbiology, July 1, 2003; 149(7): 1745 - 1752.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. T. K. Janoo, L. A. Neely, B. R. Braun, S. K. Whitehall, and C. S. Hoffman
Transcriptional Regulators of the Schizosaccharomyces pombe fbp1 Gene Include Two Redundant Tup1p-like Corepressors and the CCAAT Binding Factor Activation Complex
Genetics, March 1, 2001; 157(3): 1205 - 1215.
[Abstract] [Full Text]

Home page
 GeneticsHome page
R. M. Welton and C. S. Hoffman
Glucose Monitoring in Fission Yeast via the gpa2 G{alpha}, the git5 G{beta} and the git3 Putative Glucose Receptor
Genetics, October 1, 2000; 156(2): 513 - 521.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
L. A. Neely and C. S. Hoffman
Protein Kinase A and Mitogen-Activated Protein Kinase Pathways Antagonistically Regulate Fission Yeast fbp1 Transcription by Employing Different Modes of Action at Two Upstream Activation Sites
Mol. Cell. Biol., September 1, 2000; 20(17): 6426 - 6434.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
S. Heiland, N. Radovanovic, M. Höfer, J. Winderickx, and H. Lichtenberg
Multiple Hexose Transporters of Schizosaccharomyces pombe
J. Bacteriol., April 15, 2000; 182(8): 2153 - 2162.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1997