spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zarzov, P.
Right arrow Articles by Mann, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zarzov, P.
Right arrow Articles by Mann, C.

Journal of Cell Science, Vol 110, Issue 16 1879-1891, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication

P Zarzov, H Boucherie and C Mann
Service de Biochemie et de Genetique Moleculaire, CEA/Saclay, Gif-sur-Yvette, France.

Cdc28 is a cyclin-dependent protein kinase of Saccharomyces cerevisiae that is required for the G1/S and G2/M transitions of the cell division cycle. All previously described cdc28 mutants aside from cdc28-1N arrest division specifically in the G1 phase. cdc28-1N arrests division in G2/mitosis. We show here that the cdc28-109 mutant exhibits a mixed cell division arrest at 37 degrees C with cells in both the G1 and G2 phases. In order to identify proteins that functionally interact with Cdc28, we isolated mutants that are colethal with cdc28-109 at its permissive temperature. We describe here our phenotypic analysis of two such mutants, hsf1-82 and ydj1-10, that affect the heat shock transcription factor and a yeast dnaj-like protein chaperone, respectively. hsf1-82 and ydj1-10 temperature-sensitive mutants arrest the cell division cycle at several stages. However, one predominant class of cells in both mutants was arrested with a large bud and a single vertex of microtubules. Electron microscopic analysis of such hsf1-82 cells showed that they contained an unduplicated spindle pole body with an enlarged half-bridge. Two-dimensional gel electrophoresis of total cell proteins revealed that the hsf1-82 cells were specifically defective in the expression of the Hsc82 and Hsp82 proteins. Furthermore, the hsf1-82 mutation was suppressed by the HSC82 gene on a multicopy plasmid that restored Hsc82 protein to high levels in these cells. These results show that Hsf1 is required for spindle pole body duplication at 37 degrees C.


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. Thompson, N. J. Croft, A. Sotiriou, H. D. Piggins, and S. K. Crosthwaite
Neurospora crassa heat shock factor 1 Is an Essential Gene; a Second Heat Shock Factor-Like Gene, hsf2, Is Required for Asexual Spore Formation
Eukaryot. Cell, September 1, 2008; 7(9): 1573 - 1581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Hashikawa, N. Yamamoto, and H. Sakurai
Different Mechanisms Are Involved in the Transcriptional Activation by Yeast Heat Shock Transcription Factor through Two Different Types of Heat Shock Elements
J. Biol. Chem., April 6, 2007; 282(14): 10333 - 10340.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. W. Truman, S. H. Millson, J. M. Nuttall, M. Mollapour, C. Prodromou, and P. W. Piper
In the Yeast Heat Shock Response, Hsf1-Directed Induction of Hsp90 Facilitates the Activation of the Slt2 (Mpk1) Mitogen-Activated Protein Kinase Required for Cell Integrity
Eukaryot. Cell, April 1, 2007; 6(4): 744 - 752.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
H. Imazu and H. Sakurai
Saccharomyces cerevisiae Heat Shock Transcription Factor Regulates Cell Wall Remodeling in Response to Heat Shock
Eukaryot. Cell, June 1, 2005; 4(6): 1050 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Bandhakavi, R. O. McCann, D. E. Hanna, and C. V. C. Glover
A Positive Feedback Loop between Protein Kinase CKII and Cdc37 Promotes the Activity of Multiple Protein Kinases
J. Biol. Chem., January 24, 2003; 278(5): 2829 - 2836.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-D. Liu, K. A. Morano, and D. J. Thiele
The Yeast Hsp110 Family Member, Sse1, Is an Hsp90 Cochaperone
J. Biol. Chem., September 17, 1999; 274(38): 26654 - 26660.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. K. Lewis, J. W. Westmoreland, and M. A. Resnick
Repair of Endonuclease-Induced Double-Strand Breaks in Saccharomyces cerevisiae: Essential Role for Genes Associated with Nonhomologous End-Joining
Genetics, August 1, 1999; 152(4): 1513 - 1529.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
M. H. Jones, J. B. Bachant, A. R. Castillo, T. H. Giddings Jr., and M. Winey
Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase
Mol. Biol. Cell, July 1, 1999; 10(7): 2377 - 2391.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
K. A. Morano, N. Santoro, K. A. Koch, and D. J. Thiele
A trans-Activation Domain in Yeast Heat Shock Transcription Factor Is Essential for Cell Cycle Progression during Stress
Mol. Cell. Biol., January 1, 1999; 19(1): 402 - 411.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Oka, M. Nakai, T. Endo, C. R. Lim, Y. Kimata, and K. Kohno
Loss of Hsp70-Hsp40 Chaperone Activity Causes Abnormal Nuclear Distribution and Aberrant Microtubule Formation in M-phase of Saccharomyces cerevisiae
J. Biol. Chem., November 6, 1998; 273(45): 29727 - 29737.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1997