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Journal of Cell Science, Vol 109, Issue 7 1919-1925, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
P Dal Santo, B Blanchard and CS Hoffman
Department of Biology, Boston College, Chestnut Hill, MA 02167, USA.
The Schizosaccharomyces pombe pyp1+ gene, encoding a protein tyrosine phosphatase (pyp1), was isolated as a high copy number suppressor of a mutation that results in reduced cAMP-dependent protein kinase (PKA) activity. Overexpression of pyp1+ inhibits both transcription of the fbp1 gene, which is negatively regulated by a glucose-induced activation of PKA, and sexual development, which is negatively regulated by PKA through a nitrogen- and glucose-monitoring mechanism. Overexpression of a catalytically inactive form of pyp1 has little effect on either process. Previous studies suggest that overexpression of pyp1+ results in a mitotic delay by positively regulating wee1 activity. We show that pyp1 repression of fbp1 transcription is independent of wee1. The direct role of the pyp1 protein is to dephosphorylate and inactivate the sty1/spc1 mitogen-activated protein kinase (MAPK) that is activated by the wis1 MAPK kinase. As overexpression of pyp1+ has no further effect upon the mitotic delay observed in a wis1 deletion strain, the role of pyp1 appears to be restricted to negative regulation of the sty1/spc1 MAPK. This study indicates that pyp1 negatively regulates fbp1 transcription, sexual development and mitosis by inactivation of the sty1/spc1 MAPK, but that bifurcations downstream of the MAPK separate these processes as seen by the differential role for the wee1 gene.
This article has been cited by other articles:
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R. S. Kao, E. Morreale, L. Wang, F. D. Ivey, and C. S. Hoffman Schizosaccharomyces pombe Git1 Is a C2-Domain Protein Required for Glucose Activation of Adenylate Cyclase Genetics, May 1, 2006; 173(1): 49 - 61. [Abstract] [Full Text] [PDF]
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L. Wang, K. Griffiths Jr., Y. H. Zhang, F. D. Ivey, and C. S. Hoffman Schizosaccharomyces pombe Adenylate Cyclase Suppressor Mutations Suggest a Role for cAMP Phosphodiesterase Regulation in Feedback Control of Glucose/cAMP Signaling Genetics, December 1, 2005; 171(4): 1523 - 1533. [Abstract] [Full Text] [PDF]
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 J. Stiefel, L. Wang, D. A. Kelly, R. T. K. Janoo, J. Seitz, S. K. Whitehall, and C. S. Hoffman Suppressors of an Adenylate Cyclase Deletion in the Fission Yeast Schizosaccharomyces pombe Eukaryot. Cell, June 1, 2004; 3(3): 610 - 619. [Abstract] [Full Text] [PDF]
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K. Schadick, H. M. Fourcade, P. Boumenot, J. J. Seitz, J. L. Morrell, L. Chang, K. L. Gould, J. F. Partridge, R. C. Allshire, K. Kitagawa, et al. Schizosaccharomyces pombe Git7p, a Member of the Saccharomyces cerevisiae Sgt1p Family, Is Required for Glucose and Cyclic AMP Signaling, Cell Wall Integrity, and Septation Eukaryot. Cell, August 1, 2002; 1(4): 558 - 567. [Abstract] [Full Text] [PDF]
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S. Landry and C. S. Hoffman The git5 G{beta} and git11 G{{gamma}} Form an Atypical G{beta}{{gamma}} Dimer Acting in the Fission Yeast Glucose/cAMP Pathway Genetics, March 1, 2001; 157(3): 1159 - 1168. [Abstract] [Full Text]
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R. T. K. Janoo, L. A. Neely, B. R. Braun, S. K. Whitehall, and C. S. Hoffman Transcriptional Regulators of the Schizosaccharomyces pombe fbp1 Gene Include Two Redundant Tup1p-like Corepressors and the CCAAT Binding Factor Activation Complex Genetics, March 1, 2001; 157(3): 1205 - 1215. [Abstract] [Full Text]
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R. M. Welton and C. S. Hoffman Glucose Monitoring in Fission Yeast via the gpa2 G{alpha}, the git5 G{beta} and the git3 Putative Glucose Receptor Genetics, October 1, 2000; 156(2): 513 - 521. [Abstract] [Full Text]
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S. Landry, M. T. Pettit, E. Apolinario, and C. S. Hoffman The Fission Yeast git5 Gene Encodes a G{beta} Subunit Required for Glucose-Triggered Adenylate Cyclase Activation Genetics, April 1, 2000; 154(4): 1463 - 1471. [Abstract] [Full Text]
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J. M. Gancedo Yeast Carbon Catabolite Repression Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 334 - 361. [Abstract] [Full Text] [PDF]
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 T Caspari Onset of gluconate-H+ symport in Schizosaccharomyces pombe is regulated by the kinases Wis1 and Pka1, and requires the gti1+ gene product J. Cell Sci., January 10, 1997; 110(20): 2599 - 2608. [Abstract] [PDF]
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S Stettler, E Warbrick, S Prochnik, S Mackie, and P Fantes The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast J. Cell Sci., January 7, 1996; 109(7): 1927 - 1935. [Abstract] [PDF]
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