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Journal of Cell Science, Vol 109, Issue 3 609-617, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells

I Kitajima, K Kawahara, N Hanyu, H Shin, T Tokioka, Y Soejima, J Tsutsui, M Ozawa, T Shimayama and I Maruyama
Department of Molecular and Laboratory Medicine, Kagoshima University, Japan.

Human T-cell leukemia virus type I (HTLV-I) Tax protein induces the expression of host cellular genes, some of which are crucial in cell proliferation and differentiation. We examined the mechanisms by which HTLV-I Tax protein induces phenotypic changes in PC12 cells. We demonstrated that the HTLV-I Tax gene induces epithelioid changes and increases cell-cell contact in PC12 cells. No change in the expression of the neural cell adhesion molecule was observed between HTLV-I Tax-expressing PC12 cells and PC12 cells transfected with a control plasmid. However, HTLV-I Tax-expressing PC12 cells demonstrated a marked change in the abundance and distribution of E-cadherin, which was concentrated at regions of cellular contact and accompanied by changes in calcium-dependent cell adhesion. Although E-cadherin is expressed at low levels in PC12 and PC12 transfected with a control plasmid cells, the steady state level of E-cadherin in tax-expressing PC12 cells increases significantly, apparently as a result of regulation at the transcriptional level. Diminished expression of Tax protein in Tax-expressing PC12 cells exposed to antisense oligonucleotides for the Tax gene suppresses E-cadherin expression and decreases cell-cell adhesion. These findings imply that HTLV-I Tax protein enhanced E-cadherin expression modulates calcium-dependent cell-cell adhesion mechanisms.


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I. Kitajima, N. Hanyu, Y. Soejima, R. Hirano, S. Arahira, S. Yamaoka, R. Yamada, I. Maruyama, and Y. Kaneda
Efficient Transfer of Synthetic Ribozymes into Cells Using Hemagglutinating Virus of Japan (HVJ)-Cationic Liposomes. APPLICATION FOR RIBOZYMES THAT TARGET HUMAN T-CELL LEUKEMIA VIRUS TYPE I tax/rex mRNA
J. Biol. Chem., October 24, 1997; 272(43): 27099 - 27106.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1996