Journal of Cell Science, Vol 108, Issue 3 975-983, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Alpha v and alpha 3 integrin subunits are associated with myofibrils during myofibrillogenesis

KA McDonald, M Lakonishok and AF Horwitz
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign 61801, USA.

The development of the myofibrillar apparatus in skeletal muscle is a process in which transmembrane linkages with adhesion molecules are implicated. Integrins are one class of transmembrane adhesion receptors which appear to mediate these interactions. Two prominent linkages are at the myotendinous junction (MTJ), which residues at the ends of the cell and connects myofibrils to the tendon, and the costameres, which encircle the girth of the cell and connect the Z-disks to the sarcolemma. In this study we report that the alpha v integrin subunit is a prominent component of the costamere. The alpha v subunit is present initially on developing myotubes in a diffuse staining pattern with some concentration along nascent myofibrils. However, it appears in a striated pattern at the costamere and inconsistently at the M-line following the striation of alpha-actinin and titin but before that of desmin. Its recruitment to preformed striation suggests that it is incorporated into a pre-existing structure. The presence of alpha v in the costamere points to a role in lateral myofibrillar anchorage. In addition, we find that the alpha 3 subunit is transiently associated with myofibrils along portions of their lengths and at their ends during myofibrillogenesis. The alpha 3 subunit staining shows a novel localization and junctional structure. As myofibrils become striated the alpha 3 integrin dissociates from the localized pattern and becomes diffuse. This suggests a possible role in the stabilization of nascent myofibrils prior to striation. Antibody-induced perturbation of adhesion mediated by the integrin beta 1 subunit in developing myotubes inhibits assembly of the sarcomeric architecture.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				H. Qadota, K. B. Mercer, R. K. Miller, K. Kaibuchi, and G. M. Benian Two LIM Domain Proteins and UNC-96 Link UNC-97/PINCH to Myosin Thick Filaments in Caenorhabditis elegans Muscle Mol. Biol. Cell, November 1, 2007; 18(11): 4317 - 4326. [Abstract] [Full Text] [PDF]

				P. G De Deyne Application of Passive Stretch and Its Implications for Muscle Fibers Physical Therapy, February 1, 2001; 81(2): 819 - 827. [Abstract] [Full Text] [PDF]

				I. Tachibana and M. E. Hemler Role of Transmembrane 4 Superfamily (TM4SF) Proteins CD9 and CD81 in Muscle Cell Fusion and Myotube Maintenance J. Cell Biol., August 23, 1999; 146(4): 893 - 904. [Abstract] [Full Text] [PDF]

				P. Martin and J. Sanes Integrins mediate adhesion to agrin and modulate agrin signaling Development, January 10, 1997; 124(19): 3909 - 3917. [Abstract] [PDF]

				C. Yao, B. Ziober, A. Sutherland, D. Mendrick, and R. Kramer Laminins promote the locomotion of skeletal myoblasts via the alpha 7 integrin receptor J. Cell Sci., January 12, 1996; 109(13): 3139 - 3150. [Abstract] [PDF]