|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 11 3485-3499, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
SW James, PM Mirabito, PC Scacheri and NR Morris
Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635, USA. sjames@cc.gettysburg.edu
The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) coordinately measuring mitotic induction and DNA content of bimE7 mutant cells; and (2) analyzing epistasis relationships between bimE7 and 16 different nim mutations. A combination of cytological and flow cytometric techniques was used to show that bimE7 cells at restrictive temperature (44 degrees C) undergo a normal, although somewhat slower cell cycle prior to mitotic arrest. Most bimE7 cells were fully reversible from restrictive temperature arrest, indicating that they are able to enter mitosis normally, and therefore require bimE function in order to finish mitosis. Furthermore, epistasis studies between bimE7 and mutations in cdc2 pathway components revealed that the induction of mitosis caused by inactivation of bimE requires functional p34cdc2 kinase, and that mitotic induction by bimE7 depends upon several other nim genes whose functions are not yet known. The involvement of bimE in S phase function and mitotic checkpoint control was suggested by three lines of evidence. First, at restrictive temperature the bimE7 mutation slowed the cell cycle by delaying the onset or execution of S phase. Second, at permissive temperature (30 degrees C) the bimE7 mutation conferred enhanced sensitivity to the DNA synthesis inhibitor hydroxyurea. Finally, the checkpoint linking M phase to the completion of S phase was abolished when bimE7 was combined with two nim mutations that cause arrest in G1 or S phase. A model for bimE function based on these findings is presented.
This article has been cited by other articles:
|
|
 |
|
  A. S. Gladfelter, N. Sustreanu, A. K. Hungerbuehler, S. Voegeli, V. Galati, and P. Philippsen The Anaphase-Promoting Complex/Cyclosome Is Required for Anaphase Progression in Multinucleated Ashbya gossypii Cells Eukaryot. Cell, February 1, 2007; 6(2): 182 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sadanandom, K. Findlay, J. H. Doonan, P. Schulze-Lefert, and K. Shirasu CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in Aspergillus nidulans Eukaryot. Cell, August 1, 2004; 3(4): 984 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Joseph, S. N. Daigle, and A. R. Means PINA Is Essential for Growth and Positively Influences NIMA Function in Aspergillus nidulans J. Biol. Chem., July 30, 2004; 279(31): 32373 - 32384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Pitt, E. Moreau, P. A. Lunness, and J. H. Doonan The pot1+ homologue in Aspergillus nidulans is required for ordering mitotic events J. Cell Sci., January 15, 2004; 117(2): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. C. De Souza, X. S. Ye, and S. A. Osmani Checkpoint Defects Leading to Premature Mitosis Also Cause Endoreplication of DNA in Aspergillus nidulans Mol. Biol. Cell, November 1, 1999; 10(11): 3661 - 3674. [Abstract] [Full Text]
|
|
|
|
|
|
X. S. Ye, R. R. Fincher, A. Tang, A. H. Osmani, and S. A. Osmani Regulation of the Anaphase-promoting Complex/Cyclosome by bimAAPC3 and Proteolysis of NIMA Mol. Biol. Cell, November 1, 1998; 9(11): 3019 - 3030. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Wu, S.A. Osmani, and P.M. Mirabito A Role for NIMA in the Nuclear Localization of Cyclin B in Aspergillus nidulans J. Cell Biol., June 29, 1998; 141(7): 1575 - 1587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. P. Efimov and N. R. Morris A Screen for Dynein Synthetic Lethals in Aspergillus nidulans Identifies Spindle Assembly Checkpoint Genes and Other Genes Involved in Mitosis Genetics, May 1, 1998; 149(1): 101 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Harris and P. R. Kraus Regulation of Septum Formation in Aspergillus nidulans by a DNA Damage Checkpoint Pathway Genetics, March 1, 1998; 148(3): 1055 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lies, J Cheng, S. James, N. Morris, M. O'Connell, and P. Mirabito BIMAAPC3, a component of the Aspergillus anaphase promoting complex/cyclosome, is required for a G2 checkpoint blocking entry into mitosis in the absence of NIMA function J. Cell Sci., January 5, 1998; 111(10): 1453 - 1465. [Abstract] [PDF]
|
|
|
|
 |
|
 P.-M. Jörgensen, E. Brundell, M. Starborg, and C. Höög A Subunit of the Anaphase-Promoting Complex Is a Centromere-Associated Protein in Mammalian Cells Mol. Cell. Biol., January 1, 1998; 18(1): 468 - 476. [Abstract] [Full Text]
|
|
|
|
|
|
Isolation of a Schizosaccharomyces pombe rad21ts Mutant That Is Aberrant in Chromosome Segregation, Microtubule Function, DNA Repair and Sensitive to Hydroxyurea: Possible Involvement of Rad21 in Ubiquitin-Mediated Proteolysis Genetics, January 1, 1998; 148(1): 49 - 58.
|
|
|
|
|
|
X. S. Ye, R. R. Fincher, A. Tang, K. K. McNeal, S. E. Gygax, A. N. Wexler, K. B. Ryan, S. W. James, and S. A. Osmani Proteolysis and Tyrosine Phosphorylation of p34cdc2/Cyclin B. THE ROLE OF MCM2 AND INITIATION OF DNA REPLICATION TO ALLOW TYROSINE PHOSPHORYLATION OF p34cdc2 J. Biol. Chem., December 26, 1997; 272(52): 33384 - 33393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ollendorff and D. J. Donoghue The Serine/Threonine Phosphatase PP5 Interacts with CDC16 and CDC27, Two Tetratricopeptide Repeat-containing Subunits of the Anaphase-promoting Complex J. Biol. Chem., December 19, 1997; 272(51): 32011 - 32018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Dayton, M. Sumi, N. N. Nanthakumar, and A. R. Means Expression of a Constitutively Active Ca2+/Calmodulin-dependent Kinase in Aspergillus nidulans Spores Prevents Germination and Entry into the Cell Cycle J. Biol. Chem., February 7, 1997; 272(6): 3223 - 3230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Reed and T. Orr-Weaver The Drosophila gene morula inhibits mitotic functions in the endo cell cycle and the mitotic cell cycle Development, January 9, 1997; 124(18): 3543 - 3553. [Abstract] [PDF]
|
|
|
|
|
|
P. J. Westfall and M. Momany Aspergillus nidulans Septin AspB Plays Pre- and Postmitotic Roles in Septum, Branch, and Conidiophore Development Mol. Biol. Cell, January 1, 2002; 13(1): 110 - 118. [Abstract] [Full Text] [PDF]
|
|
