|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 5 1197-1204, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
J Creanor and JM Mitchison
Institute of Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Scotland, UK.
H1 histone kinase activity has been followed in selection-synchronised cultures of fission yeast wild-type and wee1 mutant cells, and in induction-synchronised cells of the mutant cdc2-33. The main conclusions are: (1) in all three cases, the peak of activity is near mitosis. (2) The rise in activity is relatively slow starting in wild type at 0.4 of the cycle before mitosis. It is proposed that the beginning of the rise is the first identified event in the mitotic control. (3) The rise is twice as fast in wee and starts nearer to mitosis. (4) In all cases the beginning of the rise is in G2. (5) The fall in activity is also slow, lasting for 0.25 of the cycle, in wild type. Exit from mitosis happens well before activity has fallen to baseline. (6) In a range of size mutants, activity is roughly proportional to cell size. It is suggested that the kinase may have a cytoplasmic function. (7) Estimates have been made of the timing of mitosis in the mutants. In wee, mitosis is 0.14 of the cycle earlier than in wild type because the cells have a longer septated period at the end of the cycle. (8) A novel method has been developed for eliminating the effects of the partial asynchrony in synchronous cultures, without which the kinetic analysis would have been inaccurate.
This article has been cited by other articles:
|
|
 |
|
  V. A. Tallada, A. J. Bridge, P. A. Emery, and I. M. Hagan Suppression of the Schizosaccharomyces pombe cut12.1 Cell-Cycle Defect by Mutations in cdc25 and Genes Involved in Transcriptional and Translational Control Genetics, May 1, 2007; 176(1): 73 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. H. MacIver, K. Tanaka, A. M. Robertson, and I. M. Hagan Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe Genes & Dev., June 15, 2003; 17(12): 1507 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sveiczer, A. Csikasz-Nagy, B. Gyorffy, J. J. Tyson, and B. Novak Modeling the fission yeast cell cycle: Quantized cycle times in wee1- cdc25Delta mutant cells PNAS, July 5, 2000; 97(14): 7865 - 7870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Cerutti and V Simanis Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast J. Cell Sci., January 7, 1999; 112(14): 2313 - 2321. [Abstract] [PDF]
|
|
|
|
|
|
A Sveiczer, B Novak, and J. Mitchison Mitotic control in the absence of cdc25 mitotic inducer in fission yeast J. Cell Sci., January 4, 1999; 112(7): 1085 - 1092. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Grallert, B. Grallert, B. Ribar, and M. Sipiczki Coordination of Initiation of Nuclear Division and Initiation of Cell Division in Schizosaccharomyces pombe: Genetic Interactions of Mutations J. Bacteriol., February 15, 1998; 180(4): 892 - 900. [Abstract] [Full Text]
|
|
|
|
|
|
A Sveiczer, B Novak, and J. Mitchison The size control of fission yeast revisited J. Cell Sci., January 12, 1996; 109(12): 2947 - 2957. [Abstract] [PDF]
|
|
|
|
|
|
J Creanor and J. Mitchison The kinetics of the B cyclin p56cdc13 and the phosphatase p80cdc25 during the cell cycle of the fission yeast Schizosaccharomyces pombe J. Cell Sci., January 6, 1996; 109(6): 1647 - 1653. [Abstract] [PDF]
|
|
|
|
|
|
K Labib, S Moreno, and P Nurse Interaction of cdc2 and rum1 regulates Start and S-phase in fission yeast J. Cell Sci., January 10, 1995; 108(10): 3285 - 3294. [Abstract] [PDF]
|
|
