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Journal of Cell Science, Vol 107, Issue 12 3515-3520, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
SG Pasion, GW Brown, LM Brown and DS Ray
Molecular Biology Institute, University of California, Los Angeles 90024.
In trypanosomatids, DNA replication in the nucleus and in the single mitochondrion (or kinetoplast) initiates nearly simultaneously, suggesting that the DNA synthesis (S) phases of the nucleus and the mitochondrion are coordinately regulated. To investigate the basis for the temporal link between nuclear and mitochondrial DNA synthesis phases the expression of the genes encoding DNA ligase I, the 51 and 28 kDa subunits of replication protein A, dihydrofolate reductase and the mitochondrial type II topoisomerase were analyzed during the cell cycle progression of synchronous cultures of Crithidia fasciculata. These DNA replication genes were all expressed periodically, with peak mRNA levels occurring just prior to or at the peak of DNA synthesis in the synchronized cultures. A plasmid clone (pdN-1) in which TOP2, the gene encoding the mitochondrial topoisomerase, was disrupted by the insertion of a NEO drug-resistance cassette was found to express both a truncated TOP2 mRNA and a truncated topoisomerase polypeptide. The truncated mRNA was also expressed periodically coordinate with the expression of the endogenous TOP2 mRNA indicating that cis elements necessary for periodic expression are contained within cloned sequences. The expression of both TOP2 and nuclear DNA replication genes at the G1/S boundary suggests that regulated expression of these genes may play a role in coordinating nuclear and mitochondrial S phases in trypanosomatids.
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B. Mittra and D. S. Ray Presence of a Poly(A) Binding Protein and Two Proteins with Cell Cycle-Dependent Phosphorylation in Crithidia fasciculata mRNA Cycling Sequence Binding Protein II Eukaryot. Cell, October 1, 2004; 3(5): 1185 - 1197. [Abstract] [Full Text] [PDF]
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N. K. Avliyakulov, J. Lukes, and D. S. Ray Mitochondrial Histone-Like DNA-Binding Proteins Are Essential for Normal Cell Growth and Mitochondrial Function in Crithidia fasciculata Eukaryot. Cell, April 1, 2004; 3(2): 518 - 526. [Abstract] [Full Text] [PDF]
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N. K. Avliyakulov, J. C. Hines, and D. S. Ray Sequence Elements in both the Intergenic Space and the 3' Untranslated Region of the Crithidia fasciculata KAP3 Gene Are Required for Cell Cycle Regulation of KAP3 mRNA Eukaryot. Cell, August 1, 2003; 2(4): 671 - 677. [Abstract] [Full Text] [PDF]
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S. Taladriz, T. Hanke, M. J. Ramiro, M. Garcia-Diaz, M. Garcia de Lacoba, L. Blanco, and V. Larraga Nuclear DNA polymerase beta from Leishmania infantum. Cloning, molecular analysis and developmental regulation Nucleic Acids Res., September 15, 2001; 29(18): 3822 - 3834. [Abstract] [Full Text] [PDF]
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M. E. Drew and P. T. Englund Intramitochondrial Location and Dynamics of Crithidia fasciculata Kinetoplast Minicircle Replication Intermediates J. Cell Biol., May 7, 2001; 153(4): 735 - 744. [Abstract] [Full Text] [PDF]
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M. L. Engel, J. C. Hines, and D. S. Ray The Crithidia fasciculata RNH1 gene encodes both nuclear and mitochondrial isoforms of RNase H Nucleic Acids Res., February 1, 2001; 29(3): 725 - 731. [Abstract] [Full Text] [PDF]
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R. Mahmood, J. C. Hines, and D. S. Ray Identification of cis and trans Elements Involved in the Cell Cycle Regulation of Multiple Genes in Crithidia fasciculata Mol. Cell. Biol., September 1, 1999; 19(9): 6174 - 6182. [Abstract] [Full Text] [PDF]
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G. W. Brown and T. J. Kelly Cell cycle regulation of Dfp1, an activator of the Hsk1 protein kinase PNAS, July 20, 1999; 96(15): 8443 - 8448. [Abstract] [Full Text] [PDF]
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M. L. Engel and D. S. Ray The kinetoplast structure-specific endonuclease I is related to the 5' exo/endonuclease domain of bacterial DNA polymerase I and colocalizes with the kinetoplast topoisomerase II and DNA polymerase beta during replication PNAS, July 20, 1999; 96(15): 8455 - 8460. [Abstract] [Full Text] [PDF]
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C. E. Johnson and P. T. Englund Changes in Organization of Crithidia fasciculata Kinetoplast DNA Replication Proteins during the Cell Cycle J. Cell Biol., November 16, 1998; 143(4): 911 - 919. [Abstract] [Full Text] [PDF]
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R. Mahmood and D. S. Ray Nulear Extracts of Crithidia fasciculata Contain a Factor(s) That Binds to the 5'-Untranslated Regions of TOP2 and RPA1 mRNAs Containing Sequences Required for Their Cell Cycle Regulation J. Biol. Chem., September 11, 1998; 273(37): 23729 - 23734. [Abstract] [Full Text] [PDF]
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