spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jenkins, H.
Right arrow Articles by Hutchison, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jenkins, H.
Right arrow Articles by Hutchison, C.

Journal of Cell Science, Vol 106, Issue 1 275-285, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix

H Jenkins, T Holman, C Lyon, B Lane, R Stick and C Hutchison
Department of Biological Sciences, The University, Dundee, UK.

Xenopus egg extracts, which support nuclear assembly and DNA replication in vitro, were physically depleted of lamin B3 using monoclonal antibodies linked to magnetic beads. Depleted extracts were still able to support nuclear envelope assembly around demembranated sperm heads but the resulting pronuclei lacked a lamina and were unable to initiate semiconservative DNA replication or to assemble replicases, confirming previous data. Immunoblotting analysis of isolated nuclei and nuclear matrix fractions indicated that lamin-depleted nuclei still accumulated nucleoporins and PCNA. Furthermore, the rate of PCNA uptake was identical in lamin-depleted and control nuclei. However, neither the nucleoporins nor the PCNA was associated with nuclear matrix fractions. The major protein components of sperm pronuclear matrix fractions were characterized by two-dimensional gel electrophoresis. Of these proteins only three out of 22 species, other than the lamins, were significantly reduced in lamin-depleted nuclei, indicating that these nuclei do assemble a nuclear matrix.


This article has been cited by other articles:


Home page
 JCBHome page
D. K. Shumaker, L. Solimando, K. Sengupta, T. Shimi, S. A. Adam, A. Grunwald, S. V. Strelkov, U. Aebi, M. C. Cardoso, and R. D. Goldman
The highly conserved nuclear lamin Ig-fold binds to PCNA: its role in DNA replication
J. Cell Biol., April 21, 2008; 181(2): 269 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Dechat, K. Pfleghaar, K. Sengupta, T. Shimi, D. K. Shumaker, L. Solimando, and R. D. Goldman
Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin
Genes & Dev., April 1, 2008; 22(7): 832 - 853.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. A. Adam, K. Sengupta, and R. D. Goldman
Regulation of Nuclear Lamin Polymerization by Importin {alpha}
J. Biol. Chem., March 28, 2008; 283(13): 8462 - 8468.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. L. V. Broers, F. C. S. Ramaekers, G. Bonne, R. B. Yaou, and C. J. Hutchison
Nuclear lamins: laminopathies and their role in premature ageing.
Physiol Rev, July 1, 2006; 86(3): 967 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Krauss, C. Chen, S. Penman, and R. Heald
Nuclear actin and protein 4.1: Essential interactions during nuclear assembly in vitro
PNAS, September 16, 2003; 100(19): 10752 - 10757.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Markiewicz, T. Dechat, R. Foisner, Roy. A Quinlan, and C. J. Hutchison
Lamin A/C Binding Protein LAP2alpha Is Required for Nuclear Anchorage of Retinoblastoma Protein
Mol. Biol. Cell, December 1, 2002; 13(12): 4401 - 4413.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
O. A. Vaughan, M. Alvarez-Reyes, J. M. Bridger, J. L. V. Broers, F. C. S. Ramaekers, M. Wehnert, G. E. Morris, W. G. F. Whitfield, and C. J. Hutchison
Both emerin and lamin C depend on lamin A for localization at the nuclear envelope
J. Cell Sci., March 9, 2002; 114(14): 2577 - 2590.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. D. Goldman, Y. Gruenbaum, R. D. Moir, D. K. Shumaker, and T. P. Spann
Nuclear lamins: building blocks of nuclear architecture
Genes & Dev., March 1, 2002; 16(5): 533 - 547.
[Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. A. L. Fairley, A. Riddell, J. A. Ellis, and J. Kendrick-Jones
The cell cycle dependent mislocalisation of emerin may contribute to the Emery-Dreifuss muscular dystrophy phenotype
J. Cell Sci., January 15, 2002; 115(2): 341 - 354.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. I. Lopez-Soler, R. D. Moir, T. P. Spann, R. Stick, and R. D. Goldman
A role for nuclear lamins in nuclear envelope assembly
J. Cell Biol., July 9, 2001; 154(1): 61 - 70.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Izumi, O. A. Vaughan, C. J. Hutchison, and D. M. Gilbert
Head and/or CaaX Domain Deletions of Lamin Proteins Disrupt Preformed Lamin A and C But Not Lamin B Structure in Mammalian Cells
Mol. Biol. Cell, December 1, 2000; 11(12): 4323 - 4337.
[Abstract] [Full Text]

Home page
 JCBHome page
R. D. Moir, T. P. Spann, H. Herrmann, and R. D. Goldman
Disruption of Nuclear Lamin Organization Blocks the Elongation Phase of DNA Replication
J. Cell Biol., June 12, 2000; 149(6): 1179 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Ortega and M. DePamphilis
Nucleoskeleton and initiation of DNA replication in metazoan cells
J. Cell Sci., June 14, 1999; 111(24): 3663 - 3673.
[Abstract] [PDF]

Home page
 JCBHome page
T. M. Gant, C. A. Harris, and K. L. Wilson
Roles of LAP2 Proteins in Nuclear Assembly and DNA Replication: Truncated LAP2beta Proteins Alter Lamina Assembly, Envelope Formation, Nuclear Size, and DNA Replication Efficiency in Xenopus laevis Extracts
J. Cell Biol., March 22, 1999; 144(6): 1083 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Drummond, P. Ferrigno, C. Lyon, J. Murphy, M. Goldberg, T. Allen, C. Smythe, and C. J. Hutchison
Temporal Differences in the Appearance of NEP-B78 and an LBR-like Protein during Xenopus Nuclear Envelope Reassembly Reflect the Ordered Recruitment of Functionally Discrete Vesicle Types
J. Cell Biol., January 25, 1999; 144(2): 225 - 240.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P Collas
Nuclear envelope disassembly in mitotic extract requires functional nuclear pores and a nuclear lamina
J. Cell Sci., January 5, 1998; 111(9): 1293 - 1303.
[Abstract] [PDF]

Home page
 JCBHome page
B. Lenz-Bohme, J. Wismar, S. Fuchs, R. Reifegerste, E. Buchner, H. Betz, and B. Schmitt
Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae
J. Cell Biol., June 2, 1997; 137(5): 1001 - 1016.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. P. Spann, R. D. Moir, A. E. Goldman, R. Stick, and R. D. Goldman
Disruption of Nuclear Lamin Organization Alters the Distribution of Replication Factors and Inhibits DNA Synthesis
J. Cell Biol., March 24, 1997; 136(6): 1201 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Z. Lu, D. Sittman, D. Brown, R Munshi, and G. Leno
Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract
J. Cell Sci., January 11, 1997; 110(21): 2745 - 2758.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
D. Ellis, H Jenkins, W. Whitfield, and C. Hutchison
GST-lamin fusion proteins act as dominant negative mutants in Xenopus egg extract and reveal the function of the lamina in DNA replication
J. Cell Sci., January 10, 1997; 110(20): 2507 - 2518.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
C Zhang, H Jenkins, M. Goldberg, T. Allen, and C. Hutchison
Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract
J. Cell Sci., January 9, 1996; 109(9): 2275 - 2286.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M Goldberg, H Jenkins, T Allen, W. Whitfield, and C. Hutchison
Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts
J. Cell Sci., January 11, 1995; 108(11): 3451 - 3461.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S Chevalier, J. Tassan, R Cox, M Philippe, and C Ford
Both cdc2 and cdk2 promote S phase initiation in Xenopus egg extracts
J. Cell Sci., January 5, 1995; 108(5): 1831 - 1841.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
P Hozak, A. Sasseville, Y Raymond, and P. Cook
Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells
J. Cell Sci., January 2, 1995; 108(2): 635 - 644.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
J Murphy, C. Crompton, S Hainey, G. Codd, and C. Hutchison
The role of protein phosphorylation in the assembly of a replication competent nucleus: investigations in Xenopus egg extracts using the cyanobacterial toxin microcystin-LR
J. Cell Sci., January 1, 1995; 108(1): 235 - 244.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
C. Hutchison, J. Bridger, L. Cox, and I. Kill
Weaving a pattern from disparate threads: lamin function in nuclear assembly and DNA replication
J. Cell Sci., January 12, 1994; 107(12): 3259 - 3269.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993