spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parpura, V.
Right arrow Articles by Henderson, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parpura, V.
Right arrow Articles by Henderson, E.

Journal of Cell Science, Vol 104, Issue 2 427-432, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Three-dimensional imaging of living neurons and glia with the atomic force microscope

V Parpura, PG Haydon and E Henderson
Department of Zoology and Genetics, Iowa State University, Ames 50011.

The atomic force microscope (AFM) was used to directly image hippocampal neurons and glia. Using chemically fixed and living cells it was possible to reconstruct three-dimensional cell structure and detect sub-cellular features such as the nucleus, mitochondria and filaments. By repeatedly scanning a single living cell we observed the movement of filaments beneath the cell membrane. Furthermore, by controlling the force applied to the scanning tip, nanosurgery was performed to selectively remove cells from the culture substratum. Thus, the atomic force microscope offers the opportunity to gain three-dimensional information about living cells and to observe the behavior of cellular components by imaging through the intact cell membrane.


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
R. C. Reyes and V. Parpura
Mitochondria Modulate Ca2+-Dependent Glutamate Release from Rat Cortical Astrocytes
J. Neurosci., September 24, 2008; 28(39): 9682 - 9691.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
J. L. Huff, M. P. Lynch, S. Nettikadan, J. C. Johnson, S. Vengasandra, and E. Henderson
Label-Free Protein and Pathogen Detection Using the Atomic Force Microscope
J Biomol Screen, September 1, 2004; 9(6): 491 - 497.
[Abstract] [PDF]

Home page
 J. Neurol. Neurosurg. PsychiatryHome page
F Lehmann-Horn and K Jurkat-Rott
Nanotechnology for neuronal ion channels
J. Neurol. Neurosurg. Psychiatry, November 1, 2003; 74(11): 1466 - 1475.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Schneider, M. E. Egan, B. P. Jena, W. B. Guggino, H. Oberleithner, and J. P. Geibel
Continuous detection of extracellular ATP on living cells by using atomic force microscopy
PNAS, October 12, 1999; 96(21): 12180 - 12185.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Ros, F. Schwesinger, D. Anselmetti, M. Kubon, R. Schafer, A. Pluckthun, and L. Tiefenauer
Antigen binding forces of individually addressed single-chain Fv antibody molecules
PNAS, June 23, 1998; 95(13): 7402 - 7405.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Schneider, K. C. Sritharan, J. P. Geibel, H. Oberleithner, and B. P. Jena
Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis
PNAS, January 7, 1997; 94(1): 316 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Pietrasanta, A Schaper, and T. Jovin
Imaging subcellular structures of rat mammary carcinoma cells by scanning force microscopy
J. Cell Sci., January 9, 1994; 107(9): 2427 - 2437.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
J. Hoh and C. Schoenenberger
Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy
J. Cell Sci., January 5, 1994; 107(5): 1105 - 1114.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993