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Journal of Cell Science, Vol 103, 15-22, Copyright © 1992 by Company of Biologists

Submitted on February 14, 1992
Accepted on May 20, 1992

Nuclear F-actin: a functional component of baculovirus-infected lepidopteran cells?

LOY E. VOLKMAN 1, SALMA N. TALHOUK 2, DANIEL I. OPPENHEIMER 1, and CAROL A. CHARLTON

1 Department of Entomology, University of California, Berkeley, California 94720, USA
2 Department of Plant Biology, University of California, Berkeley, California 94720, USA

Author for correspondence

Cellular functions known to involve actin are thought to occur in the cytoplasm. Even though actin has frequently been found in the nucleus, systems well-suited for studying the function of such nuclear actin are rare. We observed filamentous (F) actin within nuclei of IPLB-Sf-21 cells infected with Autographa californica M nuclear polyhedrosis virus (AcMNPV) as detected by laser confocal microscopy using fluorescent phalloidin probes. The nuclear F-actin co-localized with the major capsid protein of the virus during normal infections. Cytochalasin D, known to interfere with nucleocapsid morphogenesis, uncoupled the apparent co-localization of F-actin and the capsid protein and inhibited infectious progeny production. Inhibition was reversible throughout infection (even in the presence of a protein synthesis inhibitor) and the nuclear co-localization of F-actin and the capsid protein was re-established upon removal of the drug. These observations suggest that nuclear Factin plays a role in virus replication, and that AcMNPV-infected cells may constitute a useful system in which to expand our understanding of nuclear actin transport and function.

Key words: Autographa californica M nuclear polyhedrosis virus, nuclear F-actin, nucleocapsid morphogenesis, cytochalasin D

Submitted on February 14, 1992
Accepted on May 20, 1992




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© The Company of Biologists Ltd 1992